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Chapter 31 - Spermatogonial Stem Cell Culture and the Future of Germline Gene Editing

from Section 4 - Laboratory Evaluation and Treatment of Male Infertility

Published online by Cambridge University Press:  06 December 2023

By
Xichen Nie ,
Jan-Bernd Stukenborg  and
Jingtao Guo
Edited by
Douglas T. Carrell ,
Alexander W. Pastuszak  and
James M. Hotaling
Douglas T. Carrell
Affiliation:
Utah Center for Reproductive Medicine
Alexander W. Pastuszak
Affiliation:
University of Utah
James M. Hotaling
Affiliation:
Utah Center for Reproductive Medicine
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Summary

Human spermatogonial stem cells (hSSCs), the only type of germline stem cells in adult men, are responsible to ensure lifetime-long sperm production in men after sex maturation. As the starting point of spermatogenesis, this cell type delicately balances its self-renewal and differentiation, which is essential for spermatogenesis to proceed continuously. A deep and systematic understanding of hSSCs is critical for treating testis-related pathology such as male infertility and testicular germ cell tumor. In this chapter, we provide a systematic review of our previous and current understanding of hSSCs, as well as the advancement of ex vivo systems to culture human germline. Ultimately, the successful establishment of an hSSC-based human germline culture system will not only greatly benefit the study of human germ cell biology by serving as a useful platform, but also provide potential therapeutic options to treat male infertility and preserve fertility for childhood cancer patients.

Keywords

spermatogonial stem cellspermatogenesismale infertility
Type
Chapter
Information
Men's Reproductive and Sexual Health Throughout the Lifespan
An Integrated Approach to Fertility, Sexual Function, and Vitality
 , pp. 244 - 250
Publisher: Cambridge University Press
Print publication year: 2023

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