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Inhibition of FASN reduces the synthesis of medium-chain fatty acids in goat mammary gland

Published online by Cambridge University Press:  09 June 2014

J. J. Zhu ,
J. Luo ,
W. Wang ,
K. Yu ,
H. B. Wang ,
H. B. Shi ,
Y. T. Sun ,
X. Z. Lin  and
J. Li
J. J. Zhu
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
J. Luo*
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
W. Wang
Affiliation:
Department of Biological Sciences, University of Alabama, Tuscaloosa, USA
K. Yu
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
H. B. Wang
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
H. B. Shi
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
Y. T. Sun
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
X. Z. Lin
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
J. Li
Affiliation:
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
*
†E-mail: luojun@nwsuaf.edu.cn
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Abstract

Fatty acid synthase (FASN) is known as a crucial enzyme of cellular de novo fatty acid synthesis in mammary gland which has been proved as the main source of short and medium-chain fatty acids of milk. However, the regulatory role of FASN in goat-specific milk fatty acids composition remains unclear. We cloned and analyzed the full-length of FASN gene from the mammary gland of Capra hircus (Xinong Saanen dairy goat) (DQ 915966). Comparative gene expression analysis suggested that FASN is predominantly expressed in fat, small intestine and mammary gland tissues, and expresses higher level at lactation period. Inhibition of FASN activity by different concentrations (0, 5, 15, 25 and 35 μM) of orlistat, a natural inhibitor of FASN, resulted in decreased expression of acetyl-CoA carboxylase α (ACCα), lipoprotein lipase and heart-type fatty acid binding protein (H-FABP) in a concentration-dependent manner in goat mammary gland epithelial cells (GMEC). Similar results were also obtained by silencing of FASN. Additionally, reduction of FASN expression also led to apparent decline of the relative content of decanoic acid (C10:0) and lauric acid (C12:0) in GMEC. Our study provides a direct evidence for inhibition of FASN reduces cellular medium-chain fatty acids synthesis in GMEC.

Keywords

goatfatty acid synthaseorlistatshRNAfatty acids composition
Type
Research Article
Information
animal , Volume 8 , Issue 9 , September 2014 , pp. 1469 - 1478
Copyright
© The Animal Consortium 2014 

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