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Effect of slaughter age and feeding system on the neutral and polar lipid composition of horse meat

Published online by Cambridge University Press:  19 July 2017

X. Belaunzaran ,
P. Lavín ,
A. R. Mantecón ,
J. K. G. Kramer  and
N. Aldai
X. Belaunzaran
Affiliation:
Lactiker Research Group, Department of Pharmacy and Food Sciences, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, Spain
P. Lavín
Affiliation:
Mountain Livestock Institute, CSIC-ULE, Finca Marzanas, 24346 Grulleros, León, Spain
A. R. Mantecón
Affiliation:
Mountain Livestock Institute, CSIC-ULE, Finca Marzanas, 24346 Grulleros, León, Spain
J. K. G. Kramer
Affiliation:
Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, N1G 5C9Canada (retired).
N. Aldai*
Affiliation:
Lactiker Research Group, Department of Pharmacy and Food Sciences, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, Spain
*
†E-mail: noelia.aldai@ehu.eus; noeliaaldai@gmail.com
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Abstract

This study was undertaken to provide a thorough analysis of the neutral lipid (NL) and polar lipid (PL) fractions of horse meat that included the content and distribution of acyl and alkenyl moieties in foals under different rearing conditions. Two groups of crossbred horses were studied; the first group was selected from suckling foals produced under grazing conditions and slaughtered at 4 months of age (n=8), and the second group was selected from concentrate-finished foals and slaughtered at 12 months of age (n=7). There were significant differences related to the age and feeding practices of foals which affected the intramuscular (IM) fat content and the fatty acid (FA) composition of NL and PL fractions. Samples from suckling foals were leaner and provided the highest content of methylation products from the plasmalogenic lipids, and total and n-3 polyunsaturated fatty acid (PUFA). By contrast, the meat from concentrate-finished foals had a higher IM fat level resulting in a greater accumulation of 16:0 and total monounsaturated FAs in the NL fraction, whereas the muscle PL fraction retained a similar FA composition between both groups. Linolenic acid was preferentially deposited in the NL fraction, but linoleic acid and the long-chain n-3 and n-6 PUFAs were incorporated into the PL fraction where they served as cell membrane constituents and in eicosanoid formation.

Keywords

foallipid fractionsdimethylacetalsn-3 fatty acidslong-chain fatty acids
Type
Research Article
Information
animal , Volume 12 , Issue 2 , February 2018 , pp. 417 - 425
Copyright
© The Animal Consortium 2017 

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References

Aldai, N, Dugan, MER, Kramer, JKG, Martínez, A, López-Campos, O, Mantecón, AR and Osoro, K 2011. Length of concentrate finishing affects the fatty acid composition of grass-fed and genetically lean beef: an emphasis on trans-18:1 and conjugated linoleic acid profiles. Animal 5, 1643–1652.Google Scholar
Alves, SP and Bessa, RJB 2009. Comparison of two gas–liquid chromatograph columns for the analysis of fatty acids in ruminant meat. Journal of Chromatography A 1216, 5130–5139.Google Scholar
Amara, S, Barouh, N, Lecomte, J, Lafont, D, Robert, S, Villeneuve, P, De Caro, A and Carrière, F 2010. Lipolysis of natural long chain and synthetic medium chain galactolipids by pancreatic lipase-related protein 2. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids 1801, 508–516.Google Scholar
American Oil Chemists’ Society (AOCS) 2008. Official methods and recommended practices of the American Oil Chemists’ Society. AOCS, Champaign, IL, USA.Google Scholar
Badiani, A, Nanni, N, Gatta, PP, Tolomelli, B and Manfredini, M 1997. Nutrient profile of horsemeat. Journal of Food Composition and Analysis 10, 254–269.CrossRefGoogle Scholar
Bannon, CD, Craske, JD and Hilliker, AE 1985. Analysis of fatty acid methyl esters with high accuracy and reliability. IV. Fats with fatty acids containing four or more carbon atoms. Journal of the American Oil Chemists’ Society 62, 1501–1507.Google Scholar
Belaunzaran, X, Bravo‐Lamas, L, Kramer, JKG, Morales, R and Aldai, N 2017. Silver ion solid‐phase extraction cartridges employing glass housings overcome the limitations observed in the GC analysis of animal lipids with low trans fatty acid content. European Journal of Lipid Science and Technology 119, 1600124.Google Scholar
Belaunzaran, X, Lavín, P, Barron, LJR, Mantecón, AR, Kramer, JKG and Aldai, N 2017. An assessment of the fatty acid composition of horse-meat available at the retail level in northern Spain. Meat Science 124, 39–47.Google Scholar
Clauss, M, Grum, C and Hatt, JM 2009. Polyunsaturated fatty acid content in adipose tissue in foregut and hindgut fermenting mammalian herbivores: a literature survey. Mammalian Biology 74, 153–158.Google Scholar
Daniel, ZCTR, Wynn, RJ, Salter, AM and Buttery, PJ 2004. Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: the role of stearoyl-CoA desaturase. Journal of Animal Science 82, 747–758.Google Scholar
De Smet, S, Raes, K and Demeyer, D 2004. Meat fatty acid composition as affected by fatness and genetic factors: a review. Animal Research 53, 81–98.Google Scholar
Delmonte, P, Fardin Kia, AR, Kramer, JKG, Mossoba, MM, Sidisky, L and Rader, JI 2011. Separation characteristics of fatty acid methyl esters using SLB-IL111, a new ionic liquid coated capillary gas chromatographic column. Journal of Chromatography A 1218, 545–554.CrossRefGoogle ScholarPubMed
Delmonte, P, Fardin-Kia, AR, Kramer, JKG, Mossoba, MM, Sidisky, L, Tyburczy, C and Rader, JI 2012. Evaluation of highly polar ionic liquid gas chromatographic column for the determination of the fatty acids in milk fat. Journal of Chromatography A 1233, 137–146.Google Scholar
Domínguez, R, Crecente, S, Borrajo, P, Agregán, R and Lorenzo, JM 2015. Effect of slaughter age on foal carcass traits and meat quality. Animal 9, 1713–1720.Google Scholar
Emken, EA, Adlof, RO, Rohwedden, WK and Gulley, RM 1992. Comparison of linoleic and linolenic metabolism in men. Influence of dietary linoleic acid. In Essential fatty acids and eicosanoids (ed. A Sinclair and R Gibson), pp. 23–25. AOCS, Champaign, IL, USA.Google Scholar
Emken, EA, Dutton, H, Rohwedder, WK, Rakoff, H, Adlof, RO, Gulley, RM and Canary, JJ 1980. Distribution of deuterium-labeled cis- and trans-12-octadecenoic acids in human plasma and lipoprotein lipids. Lipids 15, 864–871.Google Scholar
Folch, J, Lees, M and Sloane-Stanley, GH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.Google Scholar
Franco, D, Crecente, S, Vázquez, JA, Gómez, M and Lorenzo, JM 2013. Effect of cross breeding and amount of finishing diet on growth parameters, carcass and meat composition of foals slaughtered at 15 months. Meat Science 93, 547–556.Google Scholar
Franco, D, Rodríguez, E, Purriños, L, Crecente, S, Bermúdez, R and Lorenzo, JM 2011. Meat quality of ‘Galician mountain’ foals breed. Effect of sex, slaughtered age and livestock production system. Meat Science 88, 292–298.Google Scholar
Galli, C, Agradi, E and Paoletti, R 1974. The (n− 6) Pentaene:(n− 3) Hexane fatty acid ratio as an index of linolenic acid deficiency. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism 369, 142–145.Google Scholar
Hayashi, K, Takagi, T and Kitagawa, M 1983. Compositions of ether-linked lipids in livers of two species of ratfish. Bulletin of the Japanese Society of Scientific Fisheries 49, 777–782.Google Scholar
He, ML, Ishikawa, S and Hidari, H 2005. Fatty acid profiles of various muscles and adipose tissues from fattening horses in comparison with beef cattle and pigs. Asian-Australasian Journal of Animal Sciences 18, 1655–1661.Google Scholar
Hornstein, I, Crowe, PF and Hiner, R 1967. Composition of lipids in some beef muscles. Journal of Food Science 32, 650–655.Google Scholar
Horrocks, LA 1972. Content, composition, and metabolism of mammalian and avian lipids that contain ether groups. In Ether lipids, chemistry and biology (ed. F Snyder), pp. 177–272. Academic Press, NY, USA.Google Scholar
IBM SPSS 2016. IBM SPSS Statistics (Version 24) for Windows 2016. SPSS Inc., IBM Corporation, NY, USA.Google Scholar
ISO 2002. Animal feeding stuffs – determination of crude ash. Standard 5984:2002. International Organisation for Standardisation, Geneva, Switzerland.Google Scholar
ISO 2005. Animal feeding stuffs – determination of nitrogen content and calculation of crude protein content – Part 2: block digestion and steam distillation method. International Standard ISO 5983–2:2005. International Organisation for Standardisation, Geneva, Switzerland.Google Scholar
Jerónimo, E, Alves, SP, Alfaia, CM, Prates, JAM, Santos‐Silva, J and Bessa, RJB 2011. Biohydrogenation intermediates are differentially deposited between polar and neutral intramuscular lipids of lambs. European Journal of Lipid Science and Technology 113, 924–934.Google Scholar
Juaneda, P and Rocquelin, G 1985. Rapid and convenient separation of phospholipids and non phosphorus lipids from rat heart using silica cartridges. Lipids 20, 40–41.Google Scholar
Kraft, J, Kramer, JKG, Schoene, F, Chambers, JR and Jahreis, G 2008. Extensive analysis of long-chain polyunsaturated fatty acids, CLA, trans-18:1 isomers, and plasmalogenic lipids in different retail beef types. Journal of Agricultural and Food Chemistry 56, 4775–4782.Google Scholar
Kramer, JKG, Hernandez, M, Cruz-Hernandez, C, Kraft, J and Dugan, MER 2008. Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2 and 18:3 except CLA isomers of milk fat as demonstrated using Ag-ion SPE fractionation. Lipids 43, 259–273.Google Scholar
Kramer, JKG, Sehat, N, Dugan, MER, Mossoba, MM, Yurawecz, MP, Roach, JAG, Eulitz, K, Aalhus, JL, Schaefer, AL and Ku, Y 1998. Distributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion-high-performance liquid chromatography. Lipids 33, 549–558.Google Scholar
Larick, DK and Turner, BE 1989. Influence of finishing diet on the phospholipid composition and fatty acid profile of individual phospholipids in lean muscle of beef cattle. Journal of Animal Science 67, 2282–2293.Google Scholar
Lorenzo, JM, Crecente, S, Franco, D, Sarriés, MV and Gómez, M 2014. The effect of livestock production system and concentrate level on carcass traits and meat quality of foals slaughtered at 18 months of age. Animal 8, 494–503.Google Scholar
Lorenzo, JM, Fuciños, C, Purriños, L and Franco, D 2010. Intramuscular fatty acid composition of ‘Galician Mountain’ foals breed: effect of sex, slaughtered age and livestock production system. Meat Science 86, 825–831.Google Scholar
Lorenzo, JM, Sarriés, MV, Tateo, A, Polidori, P, Franco, D and Lanza, M 2014. Carcass characteristics, meat quality and nutritional value of horsemeat: a review. Meat Science 96, 1478–1488.Google Scholar
Malacarne, M, Martuzzi, F, Summer, A and Mariani, P 2002. Protein and fat composition of mare’s milk: some nutritional remarks with reference to human and cow’s milk. International Dairy Journal 12, 869–877.Google Scholar
Nagan, N and Zoeller, RA 2001. Plasmalogens: biosynthesis and functions. Progress in Lipid Research 40, 199–229.Google Scholar
Polidori, P, Pucciarelli, S, Ariani, A, Polzonetti, V and Vincenzetti, S 2015. A comparison of the carcass and meat quality of Martina Franca donkey foals aged 8 or 12 months. Meat Science 106, 6–10.Google Scholar
Pugh, EL and Kates, M 1979. Membrane-bound phospholipid desaturases. Lipids 14, 159–165.Google Scholar
Sarriés, MV and Beriain, MJ 2005. Carcass characteristics and meat quality of male and female foals. Meat Science 70, 141–152.Google Scholar
Sarriés, MV, Murray, BE, Troy, D and Beriain, MJ 2006. Intramuscular and subcutaneous lipid fatty acid profile composition in male and female foals. Meat Science 72, 475–485.Google Scholar
Siebertz, HP, Heinz, E, Linscheid, M, Joyard, J and Douce, R 1979. Characterization of lipids from chloroplast envelopes. European Journal of Biochemistry 101, 429–438.Google Scholar
Spector, AA and Yorek, MA 1985. Membrane lipid composition and cellular function. Journal of Lipid Research 26, 1015–1035.Google Scholar
Vahmani, P, Rolland, CD, Gzyl, KE and Dugan, MER 2016. Non-conjugated cis/trans 18:2 in beef fat are mainly Δ-9 desaturation products of trans-18:1 isomers. Lipids 51, 1427–1433.Google Scholar
Wood, JD, Enser, M, Fisher, AV, Nute, GR, Sheard, PR, Richardson, RI, Hughes, SI and Whittington, FM 2008. Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.Google Scholar
Wood, JD, Richardson, RI, Nute, GR, Fisher, AV, Campo, MM, Kasapidou, E, Sheard, PR and Enser, M 2003. Effects of fatty acids on meat quality: a review. Meat Science 66, 21–32.Google Scholar