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Methodology for concurrent determination of urea kinetics and the capture of recycled urea nitrogen by ruminal microbes in cattle

Published online by Cambridge University Press:  01 March 2009

T. A. Wickersham ,
E. C. Titgemeyer  and
R. C. Cochran
T. A. Wickersham
Affiliation:
Department of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506, USA
E. C. Titgemeyer*
Affiliation:
Department of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506, USA
R. C. Cochran
Affiliation:
Department of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506, USA
*
E-mail: etitgeme@ksu.edu
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Abstract

We measured the incorporation of recycled urea-nitrogen (N) by ruminal microbes, using five ruminally and duodenally fistulated steers (237 kg) fed low-quality grass hay (47 g crude protein/kg dry matter (DM)). Three received 1 kg/day of soybean meal (SBM) and two received no supplemental protein (control). The experiment was 15 days long. Background enrichments of 15N were measured on day 9 and continuous jugular infusion of 0.12 g/day [15N15N]urea began on day 10. Daily samples of urine, feces, ruminal bacteria and duodenal digesta from days 10 through 14 were used to determine plateaus in 15N enrichment. Duodenal and bacterial samples collected on day 15 were used to measure duodenal N flows. Bacterial N flow was calculated as duodenal N flow multiplied by duodenal 15N enrichment divided by bacterial 15N enrichment. Bacterial N from recycled urea-N was calculated as bacterial N flow multiplied by bacterial 15N enrichment divided by urinary urea 15N enrichment. Urinary enrichment of [15N15N]urea plateaued within 24 h, whereas 14N15N urea plateaued within 48 h of [15N15N]urea infusion. Bacteria reached a plateau in 15N enrichment within 24 h and duodenal samples within 48 h. Urea production was 17.6 g of urea-N/day for control and 78.0 g/day for SBM. Gut entry was 0.99 g of urea-N/g of urea-N produced for control and 0.87 g/g for SBM. Incorporation of recycled N into microbial N was 9.0 g of N/day for control and 23.0 g/day for SBM. Recycled urea-N accounted for 0.33 g of N/g of microbial N at the duodenum for control and 0.27 g/g for SBM. Our methods allowed measurement of incorporation of recycled urea-N into ruminal microbial N.

Keywords

cattlerecyclingruminal bacteriaurea
Type
Full Paper
Information
animal , Volume 3 , Issue 3 , March 2009 , pp. 372 - 379
Copyright
Copyright © The Animal Consortium 2008

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References

Al-Dehneh, A, Huber, JT, Wanderley, R, Theurer, CB, Pessarakli, M, DeYoung, D 1997. Incorporation of recycled urea-N into ruminal bacteria flowing to the small intestine of dairy cows fed a high-grain or high-forage diet. Animal Feed Science and Technology 68, 327–338.CrossRefGoogle Scholar
Archibeque, SL, Burns, JC, Huntington, GB 2001. Urea flux in beef steers: effects of forage species and nitrogen fertilization. Journal of Animal Science 79, 1937–1943.CrossRefGoogle ScholarPubMed
Archibeque, SL, Burns, JC, Huntington, GB 2002. Nitrogen metabolism of beef steers fed endophyte-free tall fescue hay: effects of ruminally protected methionine supplementation. Journal of Animal Science 80, 1344–1351.CrossRefGoogle ScholarPubMed
Broderick, GA, Kang, JH 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science 63, 64–75.CrossRefGoogle ScholarPubMed
Cochran, RC, Galyean, ML 1994. Measurements of in vivo forage digestion by ruminants. In Forage quality, evaluation, and utilization (ed. GC Fahey, Jr., MC Collins, DR Mertens and LE Moser), pp. 613–643. ASA-CSSA-SSSA, Madison, WS, USA.Google Scholar
Huntington, GB, Burns, JC, Archibeque, SL 2007. Urea metabolism in beef steers grazing Bermudagrass, Caucasian bluestem, or gamagrass pastures varying in plant morphology, protein content, and protein composition. Journal of Animal Science 85, 1997–2004.CrossRefGoogle ScholarPubMed
Kennedy, PM, Milligan, LP 1980. The degradation and utilization of endogenous urea in the gastrointestinal tract of ruminants: a review. Canadian Journal of Animal Science 60, 205–221.CrossRefGoogle Scholar
Koster, HH, Cochran, RC, Titgemeyer, EC, Vanzant, ES, Abdelgadir, I, St-Jean, G 1996. Effect of increasing degradable intake protein on intake and digestion of low-quality, tallgrass-prairie forage by beef cows. Journal of Animal Science 74, 2473–2481.CrossRefGoogle ScholarPubMed
Lobley, GE, Bremner, D, Zuur, G 2000. Effects of diet quality on urea fates in sheep as assessed by refined, non-invasive urea kinetics. British Journal of Nutrition 84, 459–468.CrossRefGoogle ScholarPubMed
Marini, JC, Attene-Ramos, MS 2006. An improved analytical method for the determination of urea nitrogen isotopomers in biological samples utilizing continuous flow isotope ratio mass spectrometry. Rapid Communications in Mass Spectrometry 20, 3736–3740.CrossRefGoogle ScholarPubMed
Marini, JC, Van Amburgh, ME 2003. Nitrogen metabolism and recycling in Holstein heifers. Journal of Animal Science 81, 545–552.CrossRefGoogle ScholarPubMed
Marini, JC, Klein, JD, Sands, JM, Van Amburgh, ME 2004. Effect of nitrogen intake on nitrogen recycling and urea transporter abundance in lambs. Journal of Animal Science 82, 1157–1164.CrossRefGoogle ScholarPubMed
Marsh, WH, Fingerhut, B, Miller, H 1965. Automated and manual direct methods for the determination of blood urea. Clinical Chemistry 11, 624–627.CrossRefGoogle ScholarPubMed
Mathis, CP, Cochran, RC, Stokka, GL, Heldt, JS, Woods, BC, Olson, KC 1999. Impacts of increasing amounts of supplemental soybean meal on intake and digestion by beef steers and performance by beef cows consuming low-quality tallgrass-prairie forage. Journal of Animal Science 77, 3156–3162.CrossRefGoogle ScholarPubMed
Ouellet, DR, Demers, M, Zuur, G, Lobley, GE, Seoane, JR, Nolan, JV, Lapierre, H 2002. Effect of dietary fiber on endogenous nitrogen flows in lactating dairy cows. Journal of Dairy Science 85, 3013–3025.CrossRefGoogle ScholarPubMed
Read, WWC, Harrison, RA, Halliday, D 1982. A resin-based method for the preparation of molecular nitrogen for 15N analysis from urinary and plasma components. Analytical Biochemistry 123, 249–254.CrossRefGoogle ScholarPubMed
Robbins, KR, Norton, HW, Baker, DH 1979. Estimation of nutrient requirements from growth data. Journal of Nutrition 109, 1710–1714.CrossRefGoogle ScholarPubMed
Ruiz, R, Tedeschi, LO, Marini, JC, Fox, DG, Pell, AN, Jarvis, G, Russell, JB 2002. The effect of a ruminal nitrogen (N) deficiency in dairy cows: evaluation of the Cornell net carbohydrate and protein system ruminal N deficiency adjustment. Journal of Dairy Science 85, 2986–2999.CrossRefGoogle ScholarPubMed
Sarraseca, A, Milne, E, Metcalf, MJ, Lobley, GE 1998. Urea recycling in sheep: effects of intake. British Journal of Nutrition 79, 79–88.CrossRefGoogle ScholarPubMed
Stafford, SD, Cochran, RC, Vanzant, EC, and Fritz, JO 1996. Evaluation of the potential of supplements to substitute for low-quality, tallgrass-prairie forage. Journal of Animal Science 74, 639–647.CrossRefGoogle ScholarPubMed
Streeter, MN, Barron, SJ, Wagner, DG, Hibberd, CA, Owens, FN, McCollum, FT 1991. Technical note: a double L intestinal cannula for cattle. Journal of Animal Science 69, 2601–2607.CrossRefGoogle ScholarPubMed
Sunny, NE, Owens, SL, Baldwin, RLV, El-Kadi, SW, Kohn, RA, Bequette, BJ 2007. Salvage of blood urea nitrogen in sheep is highly dependent on plasma urea concentration and the efficiency of capture within the digestive tract. Journal of Animal Science 85, 1006–1013.CrossRefGoogle ScholarPubMed
Volk, RJ, Jackson, WA 1979. Preparing nitrogen gas for nitrogen-15 analysis. Analytical Chemistry 51, 463–464.CrossRefGoogle Scholar