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Iron stress and genetic response in cyanobacteria : fur genes from Synechococcus PCC 7942 and Anabaena PCC 7120

Published online by Cambridge University Press:  15 March 2009

J. A. Hernández ,
M. Artieda ,
M. L. Peleato ,
M. F. Fillat  and
M. T. Bes
J. A. Hernández
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. Artieda
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. L. Peleato
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. F. Fillat
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. T. Bes
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
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Abstract

Fur (Ferric uptake regulator) is a DNA-binding protein which represses the transcription of iron-regulated promoters using Fe2+ as a cofactor. In Gram negative bacteria, fur controls the expression of toxins and virulence factors when facing iron-stress. Iron is a key element limiting the growth and proliferation of phytoplankton. We have cloned the fur-gene from the cyanobacterium Synechococcus PCC 7942, applying the polymerase chain reaction (PCR) technique. The DNA fragments amplified encodes a Fur-type protein, which conserves the main motifs found in the family of Fur proteins. Recombinant Fur protein was successfully overproduced, reaching about 15 % of the total protein content. Also, a Fur protein was identified in the filamentous, nitrogen-fixing cyanobacterium Anabaena PCC 7120.

Keywords

ferric uptake regulator (fur)ironcyanobacteriaferric uptake regulator (fur)fercyanobactérie
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 38 , Issue 1 , March 2002 , pp. 3 - 11
Copyright
© Université Paul Sabatier, 2002

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