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Antibiotic and metal resistance of cultivable bacteria in the Antarctic sea urchin

Published online by Cambridge University Press:  05 May 2016

M. González-Aravena ,
R. Urtubia ,
K. Del Campo ,
P. Lavín ,
C.M.V.L. Wong ,
C.A. Cárdenas  and
G. González-Rocha
M. González-Aravena*
Affiliation:
Laboratorio de Biorrecursos Antárticos, Instituto Antártico Chileno, Punta Arenas 6200000, Chile
R. Urtubia
Affiliation:
Laboratorio de Biorrecursos Antárticos, Instituto Antártico Chileno, Punta Arenas 6200000, Chile
K. Del Campo
Affiliation:
Laboratorio de Investigación en Agentes Antibacterianos, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile
P. Lavín
Affiliation:
Laboratorio de Biorrecursos Antárticos, Instituto Antártico Chileno, Punta Arenas 6200000, Chile
C.M.V.L. Wong
Affiliation:
Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia
C.A. Cárdenas
Affiliation:
Laboratorio de Biorrecursos Antárticos, Instituto Antártico Chileno, Punta Arenas 6200000, Chile
G. González-Rocha
Affiliation:
Laboratorio de Investigación en Agentes Antibacterianos, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile
*
mgonzalez@inach.cl
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Abstract

In this paper we report the first characterization of cultivable bacteria obtained from the Antarctic sea urchin Sterechinus neumayeri. The coelomic fluid was obtained from a pool of sea urchins which was plated onto different media to isolate the bacteria. A total of 42 isolates of psychrotrophic and aerobic γ-Proteobacteria (59.5%), Flavobacteria (33.3%) and Actinomycetes (7.2%) were isolated and sequenced. These bacteria were exposed to heavy metals and antibiotics, where 38 strains were analysed by the minimal inhibitory concentration method. Antibiotic resistance was detected in 44% of cultivable strains, and a further 13% presented co-resistance to antibiotics and heavy metals. The genera of bacteria that showed an increased resistance and co-resistance to metals and antibiotics were Flavobacterium, Psychrobacter and Pseudomonas. Additionally, 30.9% of isolated bacterial strains contained plasmids, which are probably related to resistance and co-resistance to metals. These results indicate that sea urchin-associated bacteria could be reservoirs for antibiotic resistance genes.

Keywords

Antarcticacoelomic fluidmulti-resistanceSterechinus neumayeri
Type
Biological Sciences
Information
Antarctic Science , Volume 28 , Issue 4 , August 2016 , pp. 261 - 268
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Copyright
© Antarctic Science Ltd 2016 

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