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Detection of domoic acid, the amnesic shellfish toxin,in the digestive gland of Eledone cirrhosa and E. moschata (Cephalopoda, Octopoda) from the Portuguese coast

Published online by Cambridge University Press:  23 December 2005

Pedro R. Costa ,
Rui Rosa ,
João Pereira  and
Maria Antónia M. Sampayo
Pedro R. Costa
Affiliation:
Departamento de Ambiente Aquático, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
Rui Rosa
Affiliation:
Departamento de Inovação Tecnológica e Valorização dos Produtos da Pesca, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
João Pereira
Affiliation:
Departamento de Recursos Marinhos, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
Maria Antónia M. Sampayo
Affiliation:
Departamento de Ambiente Aquático, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
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Abstract

Domoic acid (DA), the toxin responsible for the illness known as amnesic shellfish poisoning (ASP), is an algal toxin produced naturally by some species of the diatom genus Pseudo-nitzschia. The toxin has been detected in a diverse array of marine organisms from copepods to whales. Cephalopods, which are important members of the food chain and active predators of known toxin vectors such as bivalves, crabs and some fishes, have just recently been implicated in DA transfer or accumulation. Here we present data showing detectable values of DA determined by HPLC-UV (high-performance liquid chromatography and ultraviolet detection) and confirmed by HPLC-MS (mass spectrometric detection) in two octopus species collected along the Portuguese continental coast: Eledone cirrhosa and E. moschata. Domoic acid was frequently detected in the digestive gland of E. moschata and occasionally reached concentrations exceeding 100 µg g−1. In contrast, E. cirrhosa contained lower concentrations of DA on the few occasions that it was detected. This suggests that E. moschata is a potential vector for DA transfer to higher trophic levels in the coastal marine food web, not excluding humans. These data, combined with known aspects of the life history of the species, are a necessary step towards achieving an understanding of the accumulation of phycotoxins in cephalopods.

Keywords

Domoic acidEledone moschataEledone cirrhosaAmnesic shellfish poisoningNE Atlantic
Type
Research Article
Information
Aquatic Living Resources , Volume 18 , Issue 4: Environmental Effects on Cephalopod Life History and Fisheries , October 2005 , pp. 395 - 400
Copyright
© EDP Sciences, IFREMER, IRD, 2005

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