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Cryosurvival of goldfish embryo after subzero freezing

Published online by Cambridge University Press:  15 February 2007

Kuo-chun Liu ,
Ta-ching Chou  and
Hung-du Lin
Kuo-chun Liu
Affiliation:
Biology Department, Science College, Thunghai University, Thaichung, 40704 Taiwan
Ta-ching Chou
Affiliation:
Biology Department, Science College, Thunghai University, Thaichung, 40704 Taiwan
Hung-du Lin
Affiliation:
Biology Department, Science College, Thunghai University, Thaichung, 40704 Taiwan
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Abstract

Goldfish embryos of pre-morula, somite formation, optic vesicle, heart formation and pigment formation stages were tested for cryoprotectant toxicity, using DMSO and glycerol at 10, 12 and 15% concentration. Those that showed 50% or more survival were employed for cold shock test, from 24 to 4 °C at the rate of –1 °C/min. Only embryos at the heart formation stage protected with 12% DMSO showed 60% survival and 35% hatching. A group of embryos of that stage went through a freezing and thawing test to temperatures of –15, –25 and –35 °C. They showed 30, 5 and 2% survival and 12, 3 and 1% hatching respectively.

Keywords

GoldfishCarassius auratusCyprinidaeembryocryosurvivalCarassius auratusCyprinidécryoconservationembryoncryoprotecteur
Type
Research Article
Information
Aquatic Living Resources , Volume 6 , Issue 1 , January 1993 , pp. 63 - 66
Copyright
© IFREMER-Gauthier-Villars, 1993

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