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Chronically gorging v. nibbling fat and cholesterol increases postprandial lipaemia and atheroma deposition in the New Zealand White rabbit

Published online by Cambridge University Press:  09 March 2007

Christine Juhel ,
Yan Pafumi ,
Michele Senft ,
Huguette Lafont  and
Denis Lairon
Christine Juhel*
Affiliation:
Unit 476-INSERM (Institut National de la Santé et de la Recherche Médicale), 18 avenue Mozart, 13009 Marseille, France
Yan Pafumi
Affiliation:
Unit 476-INSERM (Institut National de la Santé et de la Recherche Médicale), 18 avenue Mozart, 13009 Marseille, France
Michele Senft
Affiliation:
Unit 476-INSERM (Institut National de la Santé et de la Recherche Médicale), 18 avenue Mozart, 13009 Marseille, France
Huguette Lafont
Affiliation:
Unit 476-INSERM (Institut National de la Santé et de la Recherche Médicale), 18 avenue Mozart, 13009 Marseille, France
Denis Lairon*
Affiliation:
Unit 476-INSERM (Institut National de la Santé et de la Recherche Médicale), 18 avenue Mozart, 13009 Marseille, France
*
*Corresponding author: Dr Denis Lairon, fax +33 4 91 75 15 62, email lairon@marseille.inserm.fr
*Corresponding author: Dr Denis Lairon, fax +33 4 91 75 15 62, email lairon@marseille.inserm.fr
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Abstract

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In the present study, we compared the effects of nibbling and gorging on postprandial lipaemia and lipoproteins, hepatic lipid uptake and atheroma deposition. New Zealand White rabbits were fed on a low-fat (LF) control diet or a peanut oil- (10 g/d) and cholesterol- (0·5 g/d) enriched (HF) diet with the fat and cholesterol components given either by nibbling (HF-N) or gorging (HF-G). After 4 and 8 weeks, rabbits were given a test meal, which was either nibbled or taken as a bolus. The LF diet did not noticeably alter postprantial lipid variables. Triacylglycerol levels, 0–35 h lipid responses and plasma accumulation of dietary lipids were significantly higher in the HF-G group than in the HF-N group, despite higher post-heparin plasma lipase activities. Furthermore, as studied on cultured isolated hepatocytes, the higher the rate of supply of triacylglycerol- and cholesterol-rich lipoproteins (TCRL), the lower the rate of lipid uptake and bile salt secretion. Atheroma deposition was significantly increased by gorging the HF diet and was correlated with levels of most postprandial lipid variables. We conclude that gorging v. nibbling a fat and cholesterol-enriched diet exacerbates postprandial lipaemia by reducing the rate of TCRL clearance and favours atheroma deposition.

Keywords

Dietary lipidsHepatic lipid uptakeLipoproteins
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 5 , May 2000 , pp. 549 - 559
Copyright
Copyright © The Nutrition Society 2000

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