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Effects of dietary conjugated linoleic acid on fatty acid composition and cholesterol content of hen egg yolks

Published online by Cambridge University Press:  07 June 2007

Beata Szymczyk
Affiliation:
Department of Animal Nutrition, Institute of Animal Production, 32-083 Balice, Poland
Paweł M. Pisulewski*
Affiliation:
Department of Human Nutrition, Agricultural University of Cracow, al. 29 Listopada 46, 31-425 Kraków, Poland
*
*Corresponding author: Professor Paweł M. Pisulewski, fax +48 12 411 77 53, email rrpisule@cyf-kr.edu.pl
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Abstract

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The main objectives of the present study were to determine the effect of dietary conjugated linoleic acid (CLA) isomers on the fatty acid composition and cholesterol content of egg-yolk lipids. Forty-five 25-week-old laying hens were randomly distributed into five groups of nine hens each and maintained in individual laying cages, throughout 12 weeks of the experiment. They were assigned to the five treatments that consisted of commercial layer diets containing 0, 5, 10, 15 or 20g pure CLA/kg. Feed intake of hens varied little and insignificantly. Egg mass was uniformly lower (P<0·05) in the hens fed the CLA-enriched diets. Feed conversion efficiency, when expressed per kg eggs, was impaired (P<0·05), although without obvious relation to the dietary CLA concentration. Feeding the CLA-enriched diets resulted in gradually increasing deposition of CLA isomers (P<0·01) in egg-yolk lipids. Saturated fatty acids were increased (P<0·01) and monounsaturated fatty acids decreased (P<0·01). Polyunsaturated fatty acids (PUFA), when expressed as non-CLA PUFA, were also significantly decreased (P<0·01). The most striking effects (P<0·01) were observed for palmitic (16 : 0) and stearic (18 : 0) acids, which increased from 23·6 to 34% and from 7·8 to 18%, respectively. On the other hand, oleic acid (18 : 1n-9) decreased from 45·8 to 24·3%. Among non-CLA PUFA, linoleic (18 : 2n-6) and α-linolenic (18 : 3n-3) acids were strongly (P<0·01) decreased, from 14·2 to 7·7% and from 1·3 to 0·3%, respectively. The same was true for arachidonic (20:4n-6) and docosahexaenoic (22 : 6n-3) acids. The cholesterol content of egg yolks, when expressed in mg/g yolk, was not affected by the dietary CLA concentrations. In conclusion, unless the adverse effects of CLA feeding to laying hens on the fatty acid profile of egg yolks are eliminated, the CLA-enriched eggs cannot be considered functional food products.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2003

References

Ahn, DU, Sell, JL, Jo, C, Chamruspollert, M & Jeffrey, M (1999) Effect of dietary conjugated linoleic acid on the quality characteristics of chicken eggs during refrigerated storage. Poult Sci 78, 922928.CrossRefGoogle ScholarPubMed
Allain, CC, Poon, LS, Chan, CS, Richmind, W & Fu, PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20, 470475.CrossRefGoogle ScholarPubMed
Aydin, R, Pariza, MW & Cook, ME (2001) Olive oil prevents the adverse effects of dietary conjugated linoleic acid on chick hatchability and egg quality. J Nutr 131, 800806.CrossRefGoogle ScholarPubMed
Ayerza, R & Coates, W (2001) Omega-3 enriched eggs: The influence of dietary α-linolenic acid fatty acid source on egg production and composition. Can J Anim Sci 81, 355362.CrossRefGoogle Scholar
Baumgard, LH, Sangster, JK & Bauman, DE (2001) Milk fat synthesis in dairy cows is progressively reduced by increasing supplemental amounts of trans-10, cis-12 conjugated linoleic acid (CLA). J Nutr 131, 17641769.CrossRefGoogle ScholarPubMed
Belury, MA & Kempa-Steczko, A (1997) Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32, 199204.CrossRefGoogle ScholarPubMed
Botsoglou, NA, Yannakopoulous, AL, Fletours, DJ, Tserveni-Goussi, AS & Psomas, IE (1998) Yolk fatty acid composition and cholesterol content in response to level and form of dietary flaxseed. J Agric Food Chem 46, 46524656.CrossRefGoogle Scholar
Bretillon, L, Chardigny, JM, Gregoire, S, Berdeaux, O & Sebedio, JL (1999) Effect of conjugated linoleic acid isomers on the hepatic microsomal desaturation activities in vitro. Lipids 34, 965969.CrossRefGoogle ScholarPubMed
Chamruspollert, M & Sell, JL (1999) Transfer of dietary conjugated linoleic acid to egg yolks of chickens. Poult Sci 78, 11381150.CrossRefGoogle ScholarPubMed
Cook, HW (1991) Fatty acid desaturation and chain elongation in eucaryotes. In Biochemistry of Lipids, Lipoproteins and Membranes. pp 141169. [Vance, DE and Vance, J, editors]. New York, NY: Elsevier.Google Scholar
Dhiman, TR, Anand, GR, Satter, LD & Pariza, MW (1999) Conjugated linoleic acid content of milk from cows fed different diets. J Dairy Sci 82, 21462156.CrossRefGoogle ScholarPubMed
Du, M, Ahn, DU & Sell, JL (1999) Effect of dietary conjugated linoleic acid on the composition of egg yolk lipids. Poult Sci 78, 16391645.CrossRefGoogle ScholarPubMed
Du, M, Ahn, DU & Sell, JL (2000) Effects of conjugated linoleic acid and linoleic:linolenic acid ratio on polyunsaturated fatty acid status in laying hens. Poult Sci 79, 17491756.CrossRefGoogle ScholarPubMed
Duncan, DB (1955) Multiple range and multiple F tests. Biometrics 11, 142.CrossRefGoogle Scholar
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 479509.CrossRefGoogle ScholarPubMed
Fritche, J & Steinhart, H (1998) Analysis, occurrence, and physiological properties of trans fatty acids (TFA) with particular emphasis on conjugated linoleic acid isomers (CLA)-a review. Fett/Lipid 100, 190210.3.0.CO;2-5>CrossRefGoogle Scholar
Goodridge, J, Ingalls, JR & Crow, GH (2001) Transfer of omega-3 linolenic acid and linoleic acid to milk fat from flaxseed or Linola protected with formaldehyde. Can J Anim Sci 81, 525532.CrossRefGoogle Scholar
Ip, C, Banni, S, Angioni, E, Carta, G, McGinley, J, Thompson, HJ, Barbano, D & Bauman, D (1999) Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. J Nutr 129, 21352142.CrossRefGoogle ScholarPubMed
Jones, S, Ma, DWL, Robinson, FE, Field, CJ & Clandinin, MT (2000) Isomers of conjugated linoleic acid (CLA) are incorporated into egg yolk lipids by CLA-fed laying hens. J Nutr 130, 202205.CrossRefGoogle ScholarPubMed
Joo, ST, Lee, JI, Ha, YL & Park, GB (2002) Effects of conjugated linoleic acid on fatty acid composition, lipid oxidation, color, and water-holding capacity of pork loin. J Anim Sci 80, 108112.CrossRefGoogle ScholarPubMed
Kelly, MR, Berry, JR, Dwyer, DA, Griinari, JM, Chouinard, PY, Van Amburgh, ME & Bauman, D (1998) Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J Nutr 128, 881885.CrossRefGoogle ScholarPubMed
Kowalski, ZM, Pisulewski, PM & Spanghero, M (1999) Effects of calcium soaps of rapeseed fatty acids and protected methionine on milk yield and composition in dairy cows. J Dairy Res 66, 475487.CrossRefGoogle ScholarPubMed
Lawson, RE, Moss, AR & Givens, DI (2001) The role of dairy products in supplying linoleic acid to man's diet: a review. Nutr Res Rev 14, 153172.CrossRefGoogle ScholarPubMed
Lee, KN, Pariza, MW & Ntambi, JM (1998) Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochem Biophys Res Commun 248, 817821.CrossRefGoogle ScholarPubMed
Leskanich, CO & Noble, RC (1997) Manipulation of the n-3 polyunsaturated fatty acids composition of avian eggs and meat. World's Poultry Sci J 53, 155183.CrossRefGoogle Scholar
Lewis, NM, Seburg, S & Flanagan, NL (2000) Enriched eggs as a source of n-3 polyunsaturated fatty acids for humans. Poult Sci 79, 971974.CrossRefGoogle ScholarPubMed
Mantzioris, E, Cleland, LG, Gibson, RA, Neuman, MA, Demasi, M & James, MJ (2000) Biochemical effects of a diet containing foods enriched with n-3 fatty acids. Am J Clin Nutr 72, 4248.CrossRefGoogle ScholarPubMed
Matthews, KR, Homer, DB, Thies, F & Calder, PC (2000) Effect of whole linseed (Linum usitatissimum) in the diet of finishing pigs on growth performance and on the quality and fatty acid composition of various tissues. Br J Nutr 83, 637643.CrossRefGoogle ScholarPubMed
Morrison, WR & Smith, LM (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J Lipid Res 5, 600608.CrossRefGoogle ScholarPubMed
Noakes, M, Nestel, PJ & Clifton, PM (1996) Modifying the fatty acid profile of dairy products through feedlot technology lowers plasma cholesterol in humans consuming the products. Am J Clin Nutr 63, 4246.CrossRefGoogle ScholarPubMed
Noone, EJ, Roche, HM, Nugent, AP & Gibney, MJ (2002) The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects. Br J Nutr 88, 243251.CrossRefGoogle ScholarPubMed
Park, Y, Albright, KJ, Storkson, JM, Liu, W, Cook, ME & Pariza, MW (1999) Changes in body composition during feeding and withdrawal of dietary conjugated linoleic acid. Lipids 34, 243248.CrossRefGoogle Scholar
Raes, K, Huyghebaert, G, De Smet, S, Nollet, L, Arnouts, S & Demeyer, D (2002) The deposition of conjugated linoleic acid in eggs of laying hens fed diets varying in fat level and fatty acid profile. J Nutr 132, 182189.CrossRefGoogle ScholarPubMed
Ramsay, TG, Evock-Clover, CM, Steele, NC & Azain, MJ (2001) Dietary conjugated linoleic acid alters fatty acid composition of pig skeletal muscle and fat. J Anim Sci 79, 21522161.CrossRefGoogle ScholarPubMed
Roche, HM, Noone, E, Nugent, A & Gibney, MJ (2001) Conjugated linoleic acid: a novel therapetic agent?. Nutr Res Rev 14, 173187.CrossRefGoogle Scholar
Sandström, B, Bügel, S, Lauridsen, C, Nielsen, F, Jensen, C & Skibsted, LH (2000) Cholesterol-lowering potential in human subjects of fat from pigs fed rapeseed oil. Br J Nutr 84, 143150.CrossRefGoogle ScholarPubMed
Schaefer, RJ (2002) Lipoproteins, nutrition, and heart disease. Am J Clin Nutr 75, 191212.CrossRefGoogle ScholarPubMed
Scollan, ND, Choi, N-J, Kurt, E, Fisher, AV, Enser, M & Wood, JD (2001) Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. Br J Nutr 85, 115124.CrossRefGoogle ScholarPubMed
Simon, O, Männer, K, Schäfer, K, Sagredos, A & Eder, K (2000) Effects of conjugated linoleic acid on protein to fat proportions, fatty acids, and plasma lipids. Eur J Lipid Sci Technol 102, 402410.3.0.CO;2-T>CrossRefGoogle Scholar
Stewart, JW, Kaplan, ML & Beitz, DC (2001) Pork with a high content of polyunsaturated fatty acids lowers LDL cholesterol in women. Am J Clin Nutr 74, 179187.CrossRefGoogle ScholarPubMed
Surai, PF & Sparks, NHC (2001) Designer eggs: from improvement of egg composition to functional food. Trends Food Sci Technol 12, 716.CrossRefGoogle Scholar
Szymczyk, B & Pisulewski, PM (2002) Feeding conjugated linoleic acid-enriched egg yolks alters serum lipid profile in adult rats. Ann Anim Sci 2, 171178.Google Scholar
Szymczyk, B, Pisulewski, PM, Hanczakowski, P & Szczurek, W (2000) The effects of feeding conjugated linoleic (CLA) on rat growth, serum lipoproteins and subsequent lipid composition of selected rat tissues. J Sci Food Agric 80, 15531558.3.0.CO;2-Z>CrossRefGoogle Scholar
Szymczyk, B, Pisulewski, PM, Hanczakowski, P & Szczurek, W (2001) Effects of conjugated linoleic acid on growth performance, feed conversion efficiency, and subsequent carcass quality in broiler chickens. Br J Nutr 85, 465473.CrossRefGoogle ScholarPubMed
Van Elswyk, ME (1997) Comparison of n-3 fatty acid sources in laying hen rations for improvement of whole egg nutritional quality: a review. Br J Nutr 78(Suppl. 1), S61S69.CrossRefGoogle ScholarPubMed
Wiseman, J & Agunbiade, JA (1998) The influence of changes in dietary fat and oils on fatty acid profiles of carcass fat in finishing pigs. Livestock Prod Sci 54, 217227.CrossRefGoogle Scholar