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Inhibitory effects of sulphur compounds, copper and tungsten on nitrate reduction by mixed rumen micro-organisms

Published online by Cambridge University Press:  09 March 2007

Junichi Takahashi ,
Nobuyuki Johchi  and
Hiroshi Fujita
Junichi Takahashi
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan 080
Nobuyuki Johchi
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan 080
Hiroshi Fujita
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan 080
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Abstract

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1. The inhibitory effects of inorganic and organic sulphur-containing compounds, copper and tungsten on nitrate reduction by mixed rumen micro-organisms were investigated in two in vitro studies.

2. Coarsely strained rumen fluid from nitrate-adapted (Expt 1) or non-adapted (Expt 2) Suffolk Down wethers maintained on lucerne (Medicago sativa) cubes was used as an inoculum. In Expt 1, anaerobic incubation was carried out for 24 h for each medium supplemented with 10 mM-sodium nitrate and the following chemicals: 0, 1, 2, 3, 5, 8 and 10 mM-sodium sulphide, 1 and 10 mM-sodium sulphite, 1 and 10 mM-sodium sulphate, 1 and 10 mM-L-cysteine, 1 and 10 mM-DL-methionine, 1 mM-sodium tungstate and I mM-copper sulphate. In Expt 2, 1 and 10 mM-Na2S, 1 and 10 mM-L-cysteine, 1 mM-Na2WO4, and 1 mM-CuSO4 were added to incubation media to test for chemical inhibition of microbial reduction of nitrate.

3. In Expt 1, the amount of nitrite formed decreased with increasing concentration of sulphide-S added. The additions of L-cysteine, W and Cu suppressed nitrite formation in media from both nitrate-adapted and non-adapted sheep.

4. In contrast to the effects of sulphide, L-cysteine and W counteracted, to some degree, nitrate-induced reduction of volatile fatty acid (VFA) production. Addition of Cu to the media resulted in a further depression of VFA production.

Type
Rumen Physiology
Information
British Journal of Nutrition , Volume 61 , Issue 3 , May 1989 , pp. 741 - 748
Copyright
Copyright © The Nutrition Society 1989

References

Ammerman, C. B. & Goodrich, R. D. (1983) Advances in mineral nutrition in ruminants. Journal of Animal Science 57, 519–533.Google ScholarPubMed
Aslam, M. (1982) Differential effect of tungsten on the development of endogenous and nitrate-induced nitrate reductase activities in soybean leaves. Plant Physiology 70, 35–38.CrossRefGoogle ScholarPubMed
Azoulay, E., Puig, J. & Martins, Rosado De, Sousa, M. L. (1969) Regulation de la synthese de la nitrate-reductase chez Escherichia coli K 12. Annales de l'Institute Pasteur, Paris 117, 474–485.Google Scholar
Bray, A. C. & Till, A. R. (1975). Metabolism of sulphur in the gastro-intestinal tract. In Digestion and Metabolism in the Ruminant pp. 243–260 [McDonald, I.W. and Warner, A. C. I., editors]. Sydney: University of New England Publishing Unit.Google Scholar
Burke, K. A., Calder, K. & Lascelles, J. (1980) Effects of molybdenum and tungsten on induction of nitrate reductase and formate dehydrogenase in wild type and mutant Paracoccus denitrificans. Archives of Microbiology 126, 155–159.CrossRefGoogle ScholarPubMed
Collins, J. M. & Monty, K. J. (1973) The cysteine desulfhydrase of Salmonella typhimurium. Journal of Biological Chemistry 248, 5943–5949.CrossRefGoogle ScholarPubMed
Deaton, J. C., Solomon, E. I., Durfor, C. N., Wetherbee, P. J., Burgess, B. K. & Jacobs, D. B. (1984) Activation of nit-I nitrate reductase by ω-formate dehydrogenase. Biochemical and Biophysical Research Communications 121, 1042–1047.CrossRefGoogle Scholar
Grace, N. D. & Suttle, N. F. (1979) Some effects of sulphur intake on molybdenum metabolism in sheep. British Journal of Nutrition 41, 125–136.CrossRefGoogle ScholarPubMed
Hewitt, E. J. (1975) Assimilatory nitrate-nitrite reduction. Annual Review of Plant Physiology 26, 73–100.CrossRefGoogle Scholar
Horwitz, W. (1975) Official Methods of Analysis of the Association of Official Analytical Chemists 12th ed. Washington, DC: Association of Official Analytical Chemists.Google Scholar
Johnson, J. L., Cohen, H. J. & Pajagopalan, K. V. (1974a) Molecular basis of the biological function of molybdenum. Molybdeum-free sulfite oxidase from livers of tungsten-treated rats. Journal of Biological Chemistry 249, 5046–5055.CrossRefGoogle ScholarPubMed
Johnson, J. L., Waud, W. R., Cohen, H. J. & Pajagopalan, K. V. (1974b) Molecular basis of the biological function of molybdenum. Molybdenum-free xanthine oxidase from livers of tungsten-treated rats. Journal of Biological Chemistry 249, 5056–5061.CrossRefGoogle ScholarPubMed
Korzeniowski, A., Geurink, J. H. & Kemp, A. (1980) Nitrate poisoning in cattle. 5. The effect of tungstate on nitrite formation by rumen microbes. Netherland Journal of Agricultural Science 28, 16–19.CrossRefGoogle Scholar
Korzeniowski, A., Geurink, J. H. & Kemp, A. (1981) Nitrate poisoning in cattle. 6. Tungsten (wolfram) as a prophylactic against nitrate-nitrite intoxication in ruminants. Netherland Journal of Agricultural Science 29, 37–47.CrossRefGoogle Scholar
McDougall, E. I. (1948) Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochemical Journal 43, 99–109.CrossRefGoogle ScholarPubMed
Marais, J. P., Therion, J. J., Mackie, R. I., Kistner, A. & Dennison, C. D. (1988) Effect of nitrate and its reduction products on the growth and activity of the rumen microbial population. British Journal of Nutrition 59, 301–313.CrossRefGoogle ScholarPubMed
Martinez, A. & Church, D. C. (1970) Effect of various mineral elements on in vitro rumen cellulose digestion. Journal of Animal Science 31, 982–990.CrossRefGoogle ScholarPubMed
Nason, A. & Evans, H. J. (1953) Triphosphopyridine nucleotide-nitrate reductase in Neurospora. Journal of Biological Chemistry 202, 655–673.CrossRefGoogle ScholarPubMed
National Research Council (1988) Nutrient Requirements of Dairy Cattle 6th ed. Washington, DC: National Academy Press.Google Scholar
Oltmann, L. F., Claassen, V. P., Kastelein, P., Reijnders, W. N. M. & Stouthamar, A. H. (1979) Influence of tungstate on the formation and activities of four reductases in Proteus mirabilis. FEBS Letters 106 43 46.Google Scholar
Payne, W. J. (1973) Reduction of nitrogenous oxides by microorganisms. Bacteriological Reviews 37, 409–452.CrossRefGoogle ScholarPubMed
Prins, R. A., Cline-Theil, W., Malestein, A. & Counotte, G. H. M. (1980) Inhibition of nitrate reduction in some rumen bacteria by tungstate. Applied and Environmental Microbiology 40, 163–165.CrossRefGoogle ScholarPubMed
Saracino, L., Violet, M., Boxer, D. H. & Giordano, G. (1986) Activation in vitro of respiratory nitrate reductase of Escherichia coli K-12 grown in the presence of tungstate. European Journal of Biochemistry 158, 483–490.CrossRefGoogle Scholar
Seki-Chiba, S. & Ishimoto, M. (1977) Studies on nitrate reductase of Clostridium perfringens. Journal of Biochemistry 82, 1663–1671.CrossRefGoogle ScholarPubMed
Shen, T.-C., Ramados, C. S. & Vennesland, B. (1982) Effect of reduced pyridine nucleotides and tungstate on the in vitro insertion of molybdenum into demolybdo-nitrate reductase of Chlorella vulgaris. Biochimica et Biophysica Acta 704, 227–234.CrossRefGoogle ScholarPubMed
Stanier, G. & Davies, A. (1981) Effects of the antibiotic monensin and an inhibitor of methanogenesis on in vitro continuous rumen fermentations. British Journal of Nutrition 45, 567–578.CrossRefGoogle Scholar
Tachiki, T. & Nason, A. (1983). Preparation and properties of apoenzyme of nitrate reductases from wild-type and nit-3mutants of Neurospora crassa. Biochimca et Biophysica Acta 744, 16–22.CrossRefGoogle Scholar
Takahashi, J. (1983) Effect of intra-pregastric administration of nitrite on erythrocyte GSH and GSSG contents of the male golden hamster (Mesocricetus auratus). Comparative Biochemistry and Physiology 76B, 1–4.Google Scholar
Tillman, A. D., Sheriha, G. M. & Sirny, R. J. (1965) Nitrate reduction studies with sheep. Journal of Animal Science 24, 1140–1146.CrossRefGoogle Scholar