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Intraruminal propionate supplementation modifies hindlimb energy metabolism without changing the splanchnic release of glucose in growing lambs

Published online by Cambridge University Press:  09 March 2007

Linda Majdoub ,
Michel Vermorel  and
Isabelle Ortigues-Marty
Linda Majdoub
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint Genès Champanelle, France
Michel Vermorel
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint Genès Champanelle, France
Isabelle Ortigues-Marty*
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint Genès Champanelle, France
*
*Corresponding Author: Dr Isabelle Ortigues-Marty, fax +33 4 73 62 46 39, email ortigues@clermont.inra.fr
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Abstract

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The influence of propionate supplementation on the splanchnic metabolism of energy-yielding nutrients and the supply of glucose to the hindlimb was investigated in growing lambs. Six rumen-cannulated and multicatheterized lambs (32·2 kg), fed frozen rye grass at 690 kJ metabolizable energy intak/ per kg body weight0·75, were infused intraruminally with a salt solution (control) or with propionate solutions at 0·55 mo/ (P1) or 0·98 mo/ (P2) according to a replicated Latin square design. In the rumen fluid, supplementation decreased the acetate:propionate molar ratio from 2·36:1 to 1·37:1, without modifying the ruminal concentrations of acetate and NH4. As a result, the portal appearance of propionate increased by 51 and 72 % with P1 and P2, respectively, and that of L-LACTATE DOUBLED. ACROSS THE Liver, net extraction of propionate increased by 47 and 67 % with P1 and P2, respectively. However, the net hepatic production of glucose remained unchanged, probably as the result of a substantial rise in insulin secretion and its hepatic extraction. Overall, the net splanchnic release of acetate, glucose and butyrate was not modified while that of L-lactate increased. Despite this, the net uptake of acetate, glucose, l-lactate and non-esterified fatty acids by the hindlimb increased. Propionate probably enhanced the storage of energy-yielding nutrients in the hindlimb, despite their unchanged release by the splanchnic tissues and the unmodified insulinaemia. Regulatory mechanisms are not clear.

Keywords

PropionateSplanchnic tissuesHindlimbInsulin
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 1 , January 2003 , pp. 39 - 50
Copyright
Copyright © The Nutrition Society 2003

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