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Magnitude of ouabain-sensitive respiration in the liver of growing, lactating and starved sheep

Published online by Cambridge University Press:  09 March 2007

B. W. McBride  and
L. P. Milligan
B. W. McBride
Affiliation:
Department of Animal Science, The University of Alberta, Edmonton, Alberta T6G 2P5, Canada
L. P. Milligan
Affiliation:
Department of Animal Science, The University of Alberta, Edmonton, Alberta T6G 2P5, Canada
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Abstract

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1. Oxygen consumption and ouabain-sensitive respiration was measured for liver biopsies from lactating and non-lactating ewes and for hepatocytes isolated from mature, dry ewes. O2 consumption, ouabain-sensitive respiration and 86Rb+ uptake were also measured for hepatocytes isolated from lambs, fed adult sheep and adult sheep starved for 5 d.

2. Ouabain-sensitive respiration in the liver of ewes at peak lactation accounted for 45% of the total liver O2 consumption. This percentage was 24–37% higher (P > 0.05) than measurements made during late lactation and during the non-lactating period.

3. Total O2 consumption and ouabain-sensitive respiration rates of lamb hepatocytes were greater ( P > 0.05) than similar measurements for hepatocytes isolated from adult sheep.

4. Ouabain-sensitive 86Rb+ uptake by hepatocytes from fed sheep was up to six times greater (P > 0.05) than that by cells from starved sheep.

5. The magnitude of ouabain-sensitive respiration of hepatocytes from starved sheep was 62% lower (P > 0.05) than that for hepatocytes from fed sheep.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 54 , Issue 1 , July 1985 , pp. 293 - 303
Copyright
Copyright © The Nutrition Society 1985

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