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Modulation of oxidative stress by β-carotene in chicken embryo fibroblasts

Published online by Cambridge University Press:  09 March 2007

Susan M. Lawlor  and
Nora M. O'brien
Susan M. Lawlor
Affiliation:
Department of Nutrition, National Food Biotechnology Centre, University College, Cork, Ireland
Nora M. O'brien
Affiliation:
Department of Nutrition, National Food Biotechnology Centre, University College, Cork, Ireland
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Abstract

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The ability of β-carotene to protect against oxidative stress in vitro was assessed. Primary cultures of chicken embryo fibroblasts (CEF) were oxidatively stressed by exposure to paraquat (PQ). Activities of the antioxidant enzymes superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6) and glutathione peroxidase (GSH-Px; EC 1.11.1.9) were measured as indices of oxidative stress. CEF incubated with 0·25 mM-PQ for 18 h exhibited increased SOD and CAT activities and decreased GSH-Px activity compared with the control (P < 0·001). Incorporation of added β-carotene (0·1 μM) into 0·25 mM-PQ-treated CEF returned SOD activity to that seen in non-PQ-treated cells. β-Carotene (0·1 μM) reduced the CAT activity from that seen in PQ-treated cells and returned the GSH-Px activity to its control value thus protecting the cells against PQ-induced oxidative stress. However, at higher concentrations of β-carotene (10 μM), SOD and CAT activities increased significantly (P < 0·001) relative to non-PQ-treated cells and GSH-Px activity decreased relative to its control value. Similar trends were observed when CEF grown in β-carotene-enricbed media (0·1–10μM) were oxidatively stressed by exposure to 0·25 mM-PQ for 18 h.β-Carotene: Chicken embryo fibroblasts: Oxidative stress Antioxidant enzymes

Keywords

β-CaroteneChicken embryo fibroblastsOxidative stressAntioxidant enzymes
Type
Modulation of oxidative stress by β-carotene
Information
British Journal of Nutrition , Volume 73 , Issue 6 , June 1995 , pp. 841 - 850
Copyright
Copyright © The Nutrition Society 1995

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