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n−3 PUFA fail to affect in vivo, antigen-driven CD8+T-cell proliferation in the spleen of naïve mice

Published online by Cambridge University Press:  08 March 2007

Robert Irons  and
Kevin L. Fritsche
Robert Irons
Affiliation:
Nutritional Sciences Program, University of Missouri, Columbia, MO 65211, USA
Kevin L. Fritsche*
Affiliation:
Nutritional Sciences Program, University of Missouri, Columbia, MO 65211, USA Division of Animal Sciences, Department of Microbiology and Molecular Immunology, University of Missouri, Columbia, MO 65211, USA
*
*Corresponding author: Dr Kevin L. Fritsche, Division of Animal Sciences, 920 E. Campus Dr., 110 ASRC, Columbia, MO 65211, USA, fax 573 882 6827, email FritscheK@missouri.edu
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Abstract

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One of the most frequently reported immunomodulatory actions of n−3 PUFA is their ability to diminish in vivo lymphocyte proliferation. The purpose of this study was to determine if n−3 PUFA intake affects the kinetics or magnitude of the antigen-driven expansion of CD8+T-lymphocytes in vivo. In this study we utilized a well-characterized model of T-cell immunity (i.e. infection with the intracellular bacterium, Listeria monocytogenes). Weanling BALB/c mice were fed one of two experimental diets that differed solely in fat source. Our control diet contained lard (180g/kg) and was devoid of long-chain n−3 PUFA. The experimental diet contained 150g/kg menhaden fish oil and 30g/kg corn oil, thus providing approximately 8% of energy from long-chain n−3 PUFA. After 4 weeks, mice were infected intravenously with 106 colony-forming units of actA-deficient L. monocytogenes. Clonal expansion of antigen-specific CD8+T-cells in the spleen was measured at 5, 7, 9 and 14d post-challenge using a class I MHC tetramer loaded with the immunodominant peptide from this pathogen (i.e. Kd:LLO91–99). We report that feeding mice a diet rich in n−3 fatty acids did not significantly impact either the kinetics or magnitude of in vivo, antigen-driven expansion of CD8+T-cells. Furthermore, contraction of this T-cell population was not affected by n−3 PUFA treatment. To our knowledge this is the first time MHC tetramers have been used to investigate the influence of n−3 PUFA on in vivo CD8+T-cell proliferation.

Keywords

n−3 PUFACD8+T-cellProliferationMHC tetramerListeriaMice
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 4 , April 2006 , pp. 838 - 844
Copyright
Copyright © The Nutrition Society 2006

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