Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-10T12:35:46.772Z Has data issue: false hasContentIssue false
  • English
  • Français

Restoration of the glycogen-forming function of hepatocytes in rats with liver cirrhosis is facilitated by a high-carbohydrate diet

Published online by Cambridge University Press:  09 March 2007

Margarita V. Kudryavtseva ,
Natalia N. Besborodkina  and
Boris N. Kudryavtsev
Margarita V. Kudryavtseva*
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
Natalia N. Besborodkina
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
Boris N. Kudryavtsev
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
*
*Corresponding author: Dr Margarita Kudryavtseva, fax +7 812 247 0341, email cellpath@link.cytspb.rssi.ru
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Using cytofluorimetric and biochemical methods, the content of glycogen and its labile and stable fractions, as well as activities of glucose-6-phosphatase (EC 3.1.3.9), glycogen phosphorylase (EC 2.4.1.1) and glycogen synthase (EC 2.4.1.11) were determined in the rat liver for 6 months after chronic poisoning of the animals with CCl4 and then at 1, 3, and 6 months after the end of the poisoning. One group of rats was given a standard diet, the other, a high-carbohydrate diet. The 6-month long chronic intoxication with CCl4 was shown to produce development of typical liver cirrhosis characterized by a 2·8-fold increase in the total glycogen content in hepatocytes as compared with normal cells, by a fall in the glycogen labile fraction (from 85 to 53 % of the total glycogen) as well as by decreases in the activities of glycogen phosphorylase and glucose-6-phosphatase by 25 and 82% respectively. The structural rehabilitation occurred faster and more completely at the cellular level than at the tissue level. Functional variables of the cirrhotic liver tissue also recovered, after cessation of poisoning, faster and more completely than the liver structure at the tissue level: glycogen levels in hepatocytes fell dramatically, the labile: stable glycogen fraction ratio recovered completely, and the activity of glycogen phosphorylase rose to the level characteristic of the normal liver. Use of the high-carbohydrate diet promoted a somewhat faster and more complete recovery of hepatic structure and function.

Keywords

Liver cirrhosisGlycogenHigh-carbohydrate diet
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 6 , June 1999 , pp. 473 - 480
Copyright
Copyright © The Nutrition Society 1999

References

Anthonius, RJ, Endert, E & Sauerweinm, HP (1991) Glucose and fat metabolism during short term starvation in cirrhosis. Gastroenterology 100, 731–737.Google Scholar
Barber, A, Orrell, S & Bueding, E (1967) Association of enzyme with rat liver glycogen isolated by rate-zonal centrifugation. Journal of Biological Chemistry 242, 4040–4044.CrossRefGoogle ScholarPubMed
Diaz-Gomez, MJ, de Castro, CK, de Ferreyra, EC, D'Acosta, N, de Fenos, OM & Castro, JA (1975) Mechanistic studies on carbon tetrachloride hepatotoxicity in fasted and fed rats. Toxicology and Applied Pharmacology 32, 101–108.CrossRefGoogle Scholar
Fernandez, S, Garcia, G, Reimers, G, Hernandez, J, Lopez, B, Nieto, L & Gomez, B (1983 a) Alteraciones del metabolismo de los hidratos de carbono en la hepatica. Leveles de insulinemia (Changes in carbohydrate metabolism in the cirrhotic liver. The levels of insulinaemia). Revista Espanola de las Enfermendades del Aparato Digestivo 63, 163–171.Google Scholar
Fernandez, S, Garcia, G, Reimers, G, Hernandez, J, Lopez, B, Nieto, L & Gomez, B (1983 b) Alterationes del metabolismo de los hidratos de carbono en la cirrhosis hepatica. Hyperinsulinemia, hipersecretion o degradation deficiente? (Changes in carbohydrate metabolism in the cirrhotic liver. Hyperinsulinaemia, hypersecretion and hypodegradation?). Revista Espanola de las Enfermendades del Aparato Digestivo 63, 250–255.Google Scholar
Frederiks, WM, Fronik, GM, Marx, F & James, J (1985) Influence of nutritional state on ischemic damage in rat liver. Liver 5, 342–347.CrossRefGoogle ScholarPubMed
Harewood, MS, Proietto, J, Dudley, F & Afford, FO (1982) Insulin action and cirrhosis: insulin binding and lipogenesis in isolated adipocytes. Metabolism 31, 1241–1246.CrossRefGoogle ScholarPubMed
Hers, HG (1964) Glycogen storage disease. Advances in Metabolic Disorders. N.Y. 1, 1–44.CrossRefGoogle Scholar
Hinson, JF, Mays, JB & Cameron, AM (1983) Acetaminophen-induced hepatic glycogen depletion and hyperglycemia in mice. Biochemical Pharmacology 32, 1979–1988.CrossRefGoogle ScholarPubMed
Jennische, E (1983) Possible influence of ischemia on the hepatic membrane potential in the rat. Differences between fed and fasted rats. Acta Physiologica Scandinavica 118, 69–73.CrossRefGoogle Scholar
Kabadi, UM, Sisenstein, AB & Konda, J (1985) Elevated plasma ammonia level in hepatic cirrhosis: role of glucagon. Gastroenterology 88, 756–760.CrossRefGoogle ScholarPubMed
Keller, U, Sonnenberg, GE, Burckhardt, D & Perruchoud, A (1982) Evidence for an augmented glucagon dependence of hepatic glucose production in cirrhosis of the liver. Journal of Clinical Endocrinology and Metabolism 54, 961–968.CrossRefGoogle ScholarPubMed
Kruszynska, YG & McIntyre, N (1991) Relationship between insulin sensitivity, insulin secretion and glucose tolerance in cirrhosis. Hepatology 14, 103–111.CrossRefGoogle ScholarPubMed
Kruszynska, YG, Meyer-Albert, A, Wollen, N & McIntyre, N (1993) Energy expenditure and substrate metabolism after oral fructose in patients with cirrhosis. Journal of Hepatology 19, 241–451.CrossRefGoogle ScholarPubMed
Kudryavtseva, MV, Emelyanov, AV, Sakuta, GA & Kudryavtsev, BN (1996) Glycogen-forming function of hepatocytes under conditions of regeneration of the rat cirrhotic liver after a partial hepatectomy. Tsitologiya 38, 934–948.Google Scholar
Kudryavtseva, MV, Emelyanov, AV, Sakuta, GA, Skorina, AD, Sleptsova, LA & Kudryavtsev, BN (1992) Cytofluorimetric study of the content of glycogen and its fractions in hepatocytes of patients with liver cirrhosis of different etiologies. Tsitologiya 34, 100–107.Google Scholar
Kudryavtseva, MV, Kudryavtsev, BN & Rozanov, YM (1972) Effect of duration of periodate oxidation on intensity and specificity of PAS reaction with standard Schiff reagent and with the reagent Schiff-Auramine SO2. Tsitologiya 14, 1357–1362.Google Scholar
Kudryavtseva, MV, Kudryavtsev, BN & Rozanov, YM (1974) Two glycogen fractions in rat liver cells (cytofluorimetric study). Tsitologiya 16, 851–858.Google Scholar
Kudryavtseva, MV, Sakuta, GA, Emelyanov, AV, Sleptsova, LA, Skorina, AD & Kudryavtsev, BN (1994) Cytofluorimetric study of glycogen content and of activities of glycogen metabolism in human and animal hepatocytes in liver cirrhosis and under rehabilitation conditions. Tsitologiya 36, 200–210.Google Scholar
Kudryavtseva, MV, Zavadskaya, YE, Skorina, AD, Smirnova, SA & Kudryavtsev, BN (1983) Method of preparation of isolated human liver cells from material of vital punctate biopsies. Laborat. Delo 9, 21–22.Google Scholar
Lowry, DH, Rosebrough, NL, Farr, A & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Marchesini, G, Bianchi, GP, Cassarani, S, Dondi, C & Zoli, M (1984) Relationship of carbohydrate and amino acid metabolism in patients with liver cirrhosis. Advances in Hepatic Encephalopathy and Urea Cycle Diseases 21, 634–644.Google Scholar
Marchesini, G, Zoli, M, Dondi, C, Angiolini, A, Forlani, G, Melli, A, Bianchi, FB & Pisi, E (1982) Blood glucose and glucoregulatory hormones in liver cirrhosis: a study of 24 hour profiles and of the role of portal-systemic shunting. Gastroenterology and Clinical Biology 6, 272–278.Google ScholarPubMed
Murata, K, Ochiai, Y & Abashio, K (1985) Polydispersity of acidic glycosaminoglycan components in human liver and the changes at different stages in liver cirrhosis. Gastroenterology 89, 1248–1257.CrossRefGoogle ScholarPubMed
Nordlie, R (1979) Multifunctional glucose-6-phosphatase: cell biology. Life Science 26, 2397–2404.CrossRefGoogle Scholar
Owen, O, Rechle, F, Mazzoli, M & Kreulen, J (1981) Hepatic gut and renal substrate flux rates in patients with hepatic cirrhosis. Journal of Clinical Investigation 68, 240–252.CrossRefGoogle ScholarPubMed
Pelkonen, R, Kallio, H, Suoranta, H & Karonen, S-L (1981) Plasma insulin, C-peptide and blood glucose in portal, hepatic and peripheral veins in liver cirrhosis. Effect of intravenous tolbutamide. Acta Endocrinologica 97, 496–502.Google ScholarPubMed
Petrides, AS & de Fronzo, RA (1989) Glucose and insulin metabolism in cirrhosis. Journal of Hepatology 8, 107–114.CrossRefGoogle ScholarPubMed
Proietto, J, Alford, FP & Dudley, FJ (1980) The mechanism of the carbohydrate intolerance of cirrhosis. Journal of Clinical Endocrinology and Metabolism 51, 1030–1036.CrossRefGoogle ScholarPubMed
Pushpendran, CK, Vasanti, B, Shenoy, B & Eapen, J (1977) Effect of carbon tetrachloride on glycogen metabolism in fasted and refed mice. Indian Journal of Experimental Biology 15, 992–995.Google ScholarPubMed
Riggio, O, Merli, M, Cangiano, C, Capocaccia, R, Cascino, A, Lala, A, Leonetti, F, Mauceri, M, Pepe, M, Rossi Fanelli, F, Savioli, M, Tamburrano, G & Capocaccia, L (1982) Glucose intolerance in liver cirrhosis. Metabolism 31, 627–634.CrossRefGoogle ScholarPubMed
Rosa, F & Johnson, FB (1967) Enzyme digestion in the cytochemical demonstration of glycogen. Journal of Histology and Cytology 15, 14–21.Google ScholarPubMed
Rozanov, YM & Kudryavtsev, BN (1967) Method of fluorescence cytophotometry for quantitative determination of DNA. Tsitologiya 9, 361–367.Google Scholar
Sarkisov, DS (1987) Structural grounds of adaptation and compensation of disturbed functions. Meditsina Moscow 4, 1–56.Google Scholar
Solopaev, BP (1985) Comparative study of regeneration of normal and pathologically altered organs. In Comparative Aspects of Studies on Regeneration and Cellular Proliferation, pp. 279–281 [Solopaev, BP, editor]. Gorky, Russian Federation: Volgo Vyatskoe.Google Scholar
Swanson, MA (1950) Phosphatases of liver glucose-6-phosphatase. Journal of Biological Chemistry 186, 647–659.CrossRefGoogle Scholar