Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T11:37:10.197Z Has data issue: false hasContentIssue false
  • English
  • Français

Second meal effect: modified sham feeding does not provoke the release of stored triacylglycerol from a previous high-fat meal

Published online by Cambridge University Press:  09 March 2007

Kim G. Jackson ,
M. Denise Robertson ,
Barbara A. Fielding ,
Keith N. Frayn  and
Christine M. Williams
Kim G. Jackson*
Affiliation:
Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading RG6 6AP, UK
M. Denise Robertson
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, University of Oxford, Oxford OX2 6HE, UK
Barbara A. Fielding
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, University of Oxford, Oxford OX2 6HE, UK
Keith N. Frayn
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, University of Oxford, Oxford OX2 6HE, UK
Christine M. Williams
Affiliation:
Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading RG6 6AP, UK
*
*Corresponding author: Dr Kim G Jackson, fax +44 0118 9310080, email: K.jackson@afnovell.reading.ac.uk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The present study was carried out to determine whether cephalic stimulation, associated with eating a meal, was sufficient stimulus to provoke the release of stored triacylglycerol (TAG) from a previous high-fat meal. Ten subjects were studied on three separate occasions. Following a 12 h overnight fast, subjects were given a standard mixed test meal which contained 56 g fat. Blood samples were taken before the meal and for 5 h after the meal when the subjects were randomly allocated to receive either water (control) or were modified sham fed a low-fat (6 g fat) or moderate-fat (38 g fat) meal. Blood samples were collected for a further 3 h. Compared with the control, modified sham feeding a low- or moderate-fat meal did not provoke an early entry of TAG, analysed in either plasma or TAG-rich lipoprotein (TRL) fraction (density <1.006 kg/l). The TRL-retinyl ester data showed similar findings. A cephalic phase secretion of pancreatic polypeptide, without a significant increase in cholecystokinin levels, was observed on modified sham feeding. Although these data indicate that modified sham feeding was carried out successfully, analysis of the fat content of the expectorant showed that our subjects may have accidentally ingested a small amount of fat (0.7 g for the low-fat meal and 2.4 g for the moderate-fat meal). Nevertheless, an early TAG peak following modified sham feeding was not demonstrated in the present study, suggesting that significant ingestion of food, and not just oro-sensory stimulation, is necessary to provoke the release of any TAG stored from a previous meal.

Keywords

TriacylglycerolModified sham feedingSequential meal ingestion
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 2 , February 2001 , pp. 149 - 156
Copyright
Copyright © The Nutrition Society 2001

References

Brown, RH & Mueller-Harvey, I (1999) Evaluation of the novel soxflo technique for rapid extraction of crude fat in foods and animal feeds. Journal of the Association of Official Analytical Chemists International 82, 1369–1374.Google ScholarPubMed
Evans, K, Kuusela, PJ, Cruz, ML, Wilhelmova, I, Fielding, BA & Frayn, KN (1998) Rapid chylomicron appearance following sequential meals: effects of second meal composition. British Journal of Nutrition 79, 425–429.CrossRefGoogle ScholarPubMed
Fielding, BA, Callow, J, Owen, RM, Samra, JS, Matthews, DR & Frayn, KN (1996) Postprandial lipaemia: the origin of an early peak studied by specific dietary fatty acid intake during sequential meals. American Journal of Clinical Nutrition 63, 36–41.CrossRefGoogle ScholarPubMed
Glasbrenner, B, Dominguez-Munoz, JE, Nelson, DK, Pieramico, O, Holzworth, C, Riepl, RL & Malfertheiner, P (1994) Postprandial release of cholecystokinin and pancreatic polypeptide in health and gallstone disease: Relationships with gallbladder contraction. American Journal of Gastroenterology 89, 404–410.Google ScholarPubMed
Grundy, SM & Mok, HYI (1976) Chylomicron clearance in normal and hyperlipidemic man. Metabolism 25, 1225–1291.CrossRefGoogle ScholarPubMed
Holland, BA, Welch, AA, Unwin, ID, Buss, DH, Paul, AA & Southgate, DAT (1991) McCance and Widdowson's The Composition of Foods. 5th ed. Cambridge: Royal Society of Chemistry.Google Scholar
Jackson, KG, Robertson, MD, Deane, LO, Fielding, BA, Frayn, KN & Williams, CM (2000) The effect of modified sham feeding meals of varying fat content on postprandial triacylglycerol, insulin and glucose responses. Proceedings of the Nutrition Society 59, 14A.Google Scholar
Liverse, RJ, Masclee, AAM, Jansen, JBMJ & Lamers, CBHW (1993) Plasma cholecystokinin and pancreatic polypeptide secretion in response to bombesin, meal ingestion and modified sham feeding in lean and obese persons. International Journal of Obesity 18, 123–127.Google Scholar
Mattes, RD (1996) Oral fat exposure alters postprandial lipid metabolism in humans. American Journal of Clinical Nutrition 63, 911–917.Google ScholarPubMed
Mattes, RD (1997) Physiologic responses to sensory stimulation by food: Nutritional implications. Journal of the American Dietetic Association 97, 406–413.CrossRefGoogle ScholarPubMed
Matthews, JNS, Altman, DG, Campbell, MJ & Royston, P (1990) Analysis of serial measurements in medical research. British Medical Journal 300, 230–235.Google Scholar
Mendeloff, AI (1954) The effects of eating and of sham feeding upon the absorption of vitamin A palmitate in man. Journal of Clinical Investigation 33, 1015–1021.CrossRefGoogle ScholarPubMed
Patsch, JR, Miesenbock, G, Hopferwieser, T, Mü#hlberger, V, Knapp, E, Dunn, JK, Gotto, AM & Patsch, W (1992) Relation of triglyceride metabolism and coronary heart disease. Studies in the postprandial state. Arteriosclerosis and Thrombosis 12, 1336–1345.CrossRefGoogle ScholarPubMed
Peel, AS, Zampelas, A, Williams, CM & Gould, BJ (1993) A novel antiserum specific to apolipoprotein B-48: application in the investigation of postprandial lipidaemia in humans. Clinical Science 85, 521–524.CrossRefGoogle ScholarPubMed
Raybould, HE, Meyer, JH, Tabrizi, Y, Liddle, RA & Tso, P (1998) Inhibition of gastric emptying in response to intestinal lipid is dependent on chylomicron formation. American Journal of Physiology 274, R1834-R1838.Google Scholar
Robertson, MD, Jackson, KG, Williams, CM, Fielding, BA & Frayn, KN (2000) Modified sham feeding of a modest-fat meal suppresses plasma non-esterified fatty acids. Proceedings of the Nutrition Society 59, 123A.Google Scholar
Ruotolo, G, Zhang, H, Bentsianov, V & Le, N-A (1992) Protocol for the study of the metabolism of retinyl esters in plasma lipoproteins during postprandial lipaemia. Journal of Lipid Research 33, 1541–1549.Google Scholar
Wisé#n, O, Bjö#rvell, H, Cantor, P, Johansson, C & Theodorsson, E (1992) Plasma concentrations of regulatory peptides in obesity following modified sham feeding (MSF) and a liquid test meal. Regulatory Peptides 39, 43–54.CrossRefGoogle Scholar
Witteman, BJM, Edwards-Teunissen, K, Hopman, WPM, Jebbink, MCW, Masclee, AAM, Lamers, CBHW & Jansen, JBMJ (1994) Nutrient-specific effects of modified sham feeding on pancreatic polypeptide release. European Journal of Clinical Nutrition 48, 556–560.Google ScholarPubMed
Zilversmit, DB (1995) Atherogenic nature of triglycerides, postprandial lipidemia and triglyceride-rich remnant lipoproteins. Clinical Chemistry 41, 153–158.CrossRefGoogle ScholarPubMed