Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-26T08:44:35.912Z Has data issue: false hasContentIssue false
  • English
  • Français

Taurocholic acid adsorption during non-starch polysaccharide fermentation: an in vitro study

Published online by Cambridge University Press:  09 March 2007

Ingrid C. Gelissen  and
Martin A. Eastwood
Ingrid C. Gelissen
Affiliation:
Gastro-Intestinal Laboratory, Western General Hospital, Edinburgh EH4 2XU
Martin A. Eastwood
Affiliation:
Gastro-Intestinal Laboratory, Western General Hospital, Edinburgh EH4 2XU
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The association of radiolabelled taurocholic acid with the solid fraction of a faecal fermentation mixture was measured. A human faecal inoculum was incubated with [24-14C]taurocholic acid and several non-starch polysaccharide sources (pectin, wheat bran, ispaghula (Plantago ovata) husk and seed), glucose or a substrate-free control. Portions of fermentation mixture were taken at 0, 3, 6, 21 and 24 h and centrifuged to acquire a supernatant fraction and a pellet containing the fermentation residue. 14C was measured in supernatant fractions and pellets at all time points. Volatile fatty acids (VFA) were measured at 0 and 24 h to confirm bacterial growth. Radioactivity in the pellet increased over time for all substrates. Glucose resulted in the greatest incorporation of taurocholic acid into the pellet, followed by pectin. At 24 h the proportion of the total radioactivity found in the pellet was 92% for glucose, 79% for pectin, 60% for wheat bran, 59% for ispaghula seed, 53% for ispaghula husk and 26% for the control (mean of duplicates). Glucose and pectin produced the greatest quantity of VFA at 24 h. VFA production was highly correlated with radioactivity in the pellet (r 0·976, P <0·005). These results suggest that the bile acid binding capacity of a faecal culture mixture may be strongly influenced by the fermentability of the available substrate and hence related to bacterial metabolic activity.

Keywords

Non-starch polysaccharidesBile acidsFaecal bacteriaFermentation
Type
Non-starch polysaccharides and bile acids
Information
British Journal of Nutrition , Volume 74 , Issue 2 , August 1995 , pp. 221 - 228
Copyright
Copyright © The Nutrition Society 1995

References

REFERENCES

Anderson, J. W., Zettwoch, N., Feldman, T., Tietyen-Clark, J., Oeltgen, P. & Bishop, C. W. (1988). Cholesterol-lowering effects of psyllium hydrophilic mucilloid for hypercholesterolemic men. Archives of Internal Medicine 148, 292–296.CrossRefGoogle ScholarPubMed
Burton, R. & Manninen, V. (1982). Influence of a psyllium-based fibre preparation on faecal and serum parameters. Acta Medica Scandinavica 668, Suppl., 91–94.CrossRefGoogle ScholarPubMed
Chen, W.-J. L., Anderson, J. W. & Jennings, D. (1984). Propionate may mediate the hypocholesterolemic effects of certain soluble plant fibers in cholesterol-fed rats. Proceedings of the Society for Experimental Biology and Medicine 175, 215–218.CrossRefGoogle ScholarPubMed
Cummings, J. H. (1981). Short chain fatty acids in the human colon. Gut 22, 763–779.CrossRefGoogle ScholarPubMed
Eastwood, M. A., Brydon, W. G. & Anderson, D. M. W. (1986). The effect of the polysaccharide composition and structure of dietary fibers on cecal fermentation and fecal excretion. American Journal of Clinical Nutrition 44, 51–55.CrossRefGoogle ScholarPubMed
Eastwood, M. A. & Hamilton, D. (1968). Studies on the adsorption of bile salts to non-absorbed components of diet. Biochimica et Biophysica Acta 152, 165–173.CrossRefGoogle ScholarPubMed
Gelissen, I. C., Brodie, B. & Eastwood, M. A. (1994). Effect of Plantago ovata (psyllium) husk and seed on sterol metabolism: studies in normal and ileostomy subjects. American Journal of Clinical Nutrition 59, 395–400.CrossRefGoogle ScholarPubMed
Goering, H. K. & Van Soest, P. J. (1979). In vitro rumen digestibility determination. In Agricultural Handbook no. 379, pp. 12–15. Washington DC: Government Printing Office.Google Scholar
Hirano, S., Masuda, N., Oda, H. & Imamura, T. (1981). Transformation of bile acids by mixed microbial cultures from human feces and bile acid transforming activities of isolated bacterial strains. Microbiology and Immunology 25, 271–282.CrossRefGoogle ScholarPubMed
Hofmann, A. F. & Mysels, K. J. (1992). Bile acid solubility and precipitation in vitro and in vivo: the role of conjugation, pH, and Ca2+ ions. Journal of Lipid Research 33, 617–626.CrossRefGoogle ScholarPubMed
Illman, R. J., Storer, G. B. & Topping, D. L. (1993). White wheat flour lowers plasma cholesterol and increases cecal steroids relative to whole wheat flour, wheat bran and wheat pollard in rats. Journal of Nutrition 123, 1094–1100.Google ScholarPubMed
Levitt, M. D. (1969). Production and excretion of hydrogen gas in man. New England Journal of Medicine 281, 122–127.CrossRefGoogle ScholarPubMed
Midtvedt, T. (1974). Microbial bile acid transformation. American Journal of Clinical Nutrition 27, 1341–1347.CrossRefGoogle ScholarPubMed
Midtvedt, T. & Norman, A. (1972). Adsorption of bile acids to intestinal microorganisms. Acta Pathologica et Microbiologica Scandinavica B 80, 202–210.Google ScholarPubMed
Miettinen, T. A. & Tarpila, S. (1989). Serum lipids and cholesterol metabolism during guar gum, plantago ovata and high fibre treatments. Clinica Chimica Acta 183, 253–262.CrossRefGoogle ScholarPubMed
Sanford, P. A. (1982). Liver and secretions of pancreas and duodenum. In Digestive System Physiology, pp. 65–68. London: Edward Arnold Ltd.Google Scholar
Snedecor, G. W. & Cochran, W. G. (1980). Statistical Methods, 7th ed., p. 235. Iowa: The Iowa State University Press.Google Scholar
Spiller, G. A., Chernoff, M. C, Hill, R. A., Gates, J. E., Nassar, J. J. & Shipley, E. A. (1980). Effect of purified cellulose, pectin, and a low-residue diet on fecal volatile fatty acids, transit time and fecal weight in humans. American Journal of Clinical Nutrition 33, 754–759.CrossRefGoogle Scholar
Stephen, A. M. & Cummings, J. H. (1980). The microbial contribution to human faecal mass. Journal of Medical Microbiology 13, 45–56.CrossRefGoogle ScholarPubMed
Story, J. A. & Kritchevsky, D. (1976). Comparison of the binding of various bile acids and bile salts in vitro by several types of dietary fiber. Journal of Nutrition 106, 1292–1294.CrossRefGoogle Scholar
Topping, D. L. (1991). Soluble fiber polysaccharides: effects on plasma cholesterol and colonic fermentation. Nutrition Reviews 49, 195–203.CrossRefGoogle ScholarPubMed
Truswell, A. S. & Beynen, A. C. (1992). Dietary fibre and plasma lipids: potential for prevention and treatment of hyperlipidaemias. In Dietary Fibre - A Component of Food, pp. 295–332 [Scnweizer, T. F. & Edwards, C. A., editors]. London: Springer-Verla.CrossRefGoogle Scholar
Wright, R. S., Anderson, J. W. & Bridges, S. R. (1990). Propionate inhibits hepatocyte lipid synthesis. Proceedings of the Society for Experimental Biology and Medicine 195, 26–29.CrossRefGoogle ScholarPubMed