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Urinary excretion of catechin metabolites by human subjects after red wine consumption

Published online by Cambridge University Press:  09 March 2007

Jennifer L. Donovan ,
Sidika Kasim-Karakas ,
J. Bruce German  and
Andrew L. Waterhouse
Jennifer L. Donovan*
Affiliation:
Departments of Viticulture and Enology, University of California, Davis, California 95616, USA
Sidika Kasim-Karakas
Affiliation:
Endocrinology and Metabolism andUniversity of California, Davis, California 95616, USA
J. Bruce German
Affiliation:
Food Science and Technology, University of California, Davis, California 95616, USA
Andrew L. Waterhouse
Affiliation:
Departments of Viticulture and Enology, University of California, Davis, California 95616, USA
*
*Corresponding author: Dr Jennifer L. Donovan, present address Laboratory of Drug Disposition and Pharmacogenetics, Department of Psychiatry, Room 246N, Medical University of South Carolina, 67 President Street, Charleston, SC 29425, USA, fax +1 843 792 6318, email donovanj@musc.edu
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Abstract

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Little is known about flavonoid metabolism and excretion in man. In the present study, the urinary excretion of a major flavonoid in wine, catechin, and its metabolites, were measured after nine human subjects each consumed 120 ml red wine (RW) on one day and de-alcoholized red wine (DRW) on a separate day. Both the RW and DRW contained 120 (SEM 3) ΜMOL CATECHIN (35 MG). GC–MS ANALYSES OF THE TRIMETHYLSILYLATED DERIVATIVES OF CATECHIN AND 3″ AND 4″ METHYLCATECHIN WERE PERFORMED BEFORE AND AFTER HYDROLYSIS OF CONJUGATES BY Β-GLUCURONIDASE AND SULFATASE. BASELINE URINE SAMPLES COLLECTED PRIOR TO WINE CONSUMPTION CONTAINED 0·013 (sem 0·005) μmol catechin and metabolites. During the 8 h period following consumption of RW and DRW, 6·6 (sem 0·9) and 5·3 (sem 0·6) μmol catechin and metabolites were excreted in 893 (sem 94) and 740 (sem 101) ml urine respectively. This corresponded to 3·0–10·3 % of the dose after RW and 2·1–8·2 % of the dose after DRW. The amount of catechin and metabolites excreted in urine was 20 % higher after RW compared with DRW (P=0·06). Catechin in all urine samples was present as metabolites and there were no differences in the proportions of individual metabolites after RW and DRW. As with other flavonoids, the fate of most ingested catechin is not yet known.

Keywords

WineCatechinCatechin metabolitesUrinary excretionMan
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 1 , January 2002 , pp. 31 - 37
Copyright
Copyright © The Nutrition Society 2002

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