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Assessing mating performance of male Glossina pallidipes (Diptera: Glossinidae) using a walk-in field cage

Published online by Cambridge University Press:  09 March 2007

G.N. Mutika ,
E. Opiyo  and
A.S. Robinson
G.N. Mutika*
Affiliation:
Entomology Unit FAO/IAEA Agriculture and Biotechnology Laboratory, Agency's Laboratories, A-2444 Seibersdorf, Austria
E. Opiyo
Affiliation:
Entomology Unit FAO/IAEA Agriculture and Biotechnology Laboratory, Agency's Laboratories, A-2444 Seibersdorf, Austria
A.S. Robinson
Affiliation:
Entomology Unit FAO/IAEA Agriculture and Biotechnology Laboratory, Agency's Laboratories, A-2444 Seibersdorf, Austria
*
*Fax: +43 1 2600 28447 E-mail: G.Mutika@iaea.org
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Abstract

To monitor the quality of male tsetse for use in the sterile insect technique (SIT), a field cage test was developed and evaluated. Mating competitiveness was tested with male Glossina pallidipes Austen that emerged from pupae stored for different periods at 15°C. Control males emerged from pupae stored at 23–24°C and emerged at 26.5°C. Each sample of test males was divided into two groups with one group being irradiated at 120 Gy; the other group was not irradiated. More than 70% of the maximum possible number of mating pairs occurred in all tests. Males emerged from pupae kept at low temperature and then irradiated formed a greater proportion of mating pairs than the controls. Males emerged from pupae kept at 15°C generally started mating more quickly than the standard colony males although there was no significant difference. Insemination rates were above 99%. Pooled data indicated that mean spermathecal values for females mated with irradiated males were significantly lower than for control males. The duration of copulation varied significantly between treatment groups and was significantly longer for irradiated male flies; there was no correlation between duration of copulation and mean spermathecal value.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 91 , Issue 4 , August 2001 , pp. 281 - 287
Copyright
Copyright © Cambridge University Press 2001

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