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Characterization and polymorphism analysis of phosphoglucose isomerase gene in the fall webworm (Hyphantria cunea)

Published online by Cambridge University Press:  07 February 2012

J. Luo ,
X.-Y. Cheng ,
X. Yan ,
W.-Q. Tao ,
J.D. Holland  and
R.-M. Xu
J. Luo
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
X.-Y. Cheng*
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
X. Yan
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
W.-Q. Tao
Affiliation:
Beijing Municipal Bureau of Landscape and Forestry, 8 Yumin Middle Road, Beijing, 100029, China
J.D. Holland
Affiliation:
Department of Entomology, Purdue University, West Lafayette, IN 47907, USA
R.-M. Xu
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
*
*Author for correspondence Fax: +86-10-58807721 E-mail: chengxy@bnu.edu.cn
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Abstract

Phosphoglucose isomerase (PGI) plays an important role in energy metabolism, and it is documented that PGI exhibits an extensive polymorphism which can affect insects' fitness and adaptation. In this paper, we studied the structural characteristics and polymorphism of pgi gene in the fall webworm (Hyphantria cunea), an important invasive pest in some European and Asian countries. A 2110-bp pgi full-length cDNA encoding a polypeptide of 556 amino acids was obtained from H. cunea. The pgi full-length in the H. cunea genomic DNA was 14,332 bp with 12 exons and 11 introns, similar to the structures of pgi in other Lepidoptera species. We compared the structures of pgi in different insect species. Moreover, thirteen pgi genotypes comprised of five alleles were identified in the Chinese population. Genotypes pgi-cd, pgi-cc and pgi-ce were the most prevalent with over 70% of individuals allocated to them. Four out of five alleles were sequenced the cDNA full-length. Thirty stably variable sites were found among them with five non-synonymous mutation sites. The frequencies of alleles and genotypes were variable in different Chinese geographic subpopulations. Moreover, comparison of pgi mRNA expression levels in each stage of the moth's lifecycle showed that a high expression level was in the 6th instar larval stage, followed by that in the egg and adult stages. The results will provide a basis for further study of the role of different alleles and genotypes of PGI on fitness and adaptation of the moth H. cunea.

Keywords

phosphoglucose isomerasepgiallelegenotypepolymorphismHyphantria cunea
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 4 , August 2012 , pp. 477 - 488
Copyright
Copyright © Cambridge University Press 2012

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