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Cuticular and internal n-alkane composition of Lucilia sericata larvae, pupae, male and female imagines: application of HPLC-LLSD and GC/MS-SIM

Published online by Cambridge University Press:  25 January 2012

M. Gołębiowski ,
M. Paszkiewicz ,
A. Grubba ,
D. Gąsiewska ,
M.I. Boguś ,
E. Włóka ,
W. Wieloch  and
P. Stepnowski
M. Gołębiowski*
Affiliation:
Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
M. Paszkiewicz
Affiliation:
Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
A. Grubba
Affiliation:
Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
D. Gąsiewska
Affiliation:
Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
M.I. Boguś
Affiliation:
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland
E. Włóka
Affiliation:
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland
W. Wieloch
Affiliation:
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland
P. Stepnowski
Affiliation:
Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
*
*Author for correspondence Fax: (+48 58) 5235 472 E-mail: goleb@chem.univ.gda.pl
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Abstract

The composition of cuticular and internal n-alkanes in Lucilia sericata larvae, pupae, and male and female imagines were studied. The cuticular and internal lipid extracts were separated by HPLC-LLSD, after which the hydrocarbon fraction was identified by GC/MS in selected ion monitoring (SIM) and total ion current (TIC) modes.

The cuticular lipids of the larvae contained seven n-alkanes from C23 to C31. The major n-alkane in L. sericata larvae was C29 (42.1%). The total cuticular n-alkane content in the cuticular lipids was 31.46 μg g−1 of the insect body. The internal lipids of L. sericata larvae contained five n-alkanes ranged from C25 to C31. The most abundant compound was C27 (61.71 μg g−1 of the insect body). Eighteen n-alkanes from C14 to C31 were identified in the cuticular lipids of the pupae. The most abundant n-alkanes ranged from C25 to C31; those with odd-numbered carbon chains were particularly abundant, the major one being C29:0 (59.5%). Traces of eight cuticular n-alkanes were present. The internal lipids of L. sericata pupae contained five n-alkanes, ranging from C25 to C31. The cuticular lipids of female imagines contained 17 n-alkanes from C12 to C30. Among the cuticular n-alkanes of females, C27 (47.5%) was the most abundant compound. Four n-alkanes, with only odd-numbered carbon chains, were identified in the internal lipids of females. The lipids from both sexes of L. sericata had similar n-alkane profiles. The cuticular lipids of adult males contained 16 n-alkanes ranging from C13 to C31. C27 (47.9%) was the most abundant cuticular n-alkanes in males. The same n-alkanes only with odd-numbered carbon chains and in smaller quantities of C27 (0.1%) were also identified in the internal lipids of males.

The highest amounts of total cuticular n-alkanes were detected in males and females of L. sericata (330.4 and 158.93 μg g−1 of the insect body, respectively). The quantities of total cuticular alcohols in larvae and pupae were smaller (31.46 μg g−1 and 42.08 μg g−1, respectively). The internal n-alkane contents of larvae, pupae, and male and female imagines were significantly higher than the cuticular n-alkane contents (153.53, 99.60, 360.06 and 838.76 μg g−1 of the insect body, respectively).

Keywords

n-alkanesLucilia sericataHPLC-LLSDGC/MSSIM
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 4 , August 2012 , pp. 453 - 460
Copyright
Copyright © Cambridge University Press 2012

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