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A method for determining the age of very young tsetse flies (Diptera: Glossinidae) and an investigation of the factors determining head fluorescent levels in newly emerged adults

Published online by Cambridge University Press:  10 July 2009

Atway Msangi
Affiliation:
School of Biological Sciences, University of Wales, Bangor, UK
M.J. Lehane*
Affiliation:
School of Biological Sciences, University of Wales, Bangor, UK
*
M.J. Lehane, School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK.

Abstract

The new age determination technique for tsetse flies based on the accumulation of fluorescent pigments in the head capsule has the minor problem that it is not possible to accurately identify very young flies. In this paper we describe a technique for the identification of very young Glossina morsitans morsitans Westwood based on their high levels of abdominal fluorescence. Under our holding conditions these fall back in a linear fashion to a minimum at five days post-emergence. The variability in the levels of head fluorescence at eclosion is mainly influenced by the size of the emerging fly, with the number of blood meals taken by the female parent, and intra-uterine larval and puparial periods of the emerging flies also playing a part.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 1991

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References

Bursell, E. (1960) The measurement of size in tsetse flies (Glossina). Bulletin of Entomological Research 51, 3337.CrossRefGoogle Scholar
Challier, A. (1965) Amélioration de la méthode de détermination de l'age physiologique des glossines. Bulletin de la Societé Pathologique Exotique 58, 250259.Google Scholar
Glasgow, J.P. (1961) Selection for size in tsetse flies. Journal of Animal Ecology 30, 8794.CrossRefGoogle Scholar
Glasgow, J.P. & Bursell, E. (1961) Seasonal variations in the fat content and size of Glossina swynnertoni Austen. Bulletin of Entomological Research 51, 705713.CrossRefGoogle Scholar
Jordan, A.M. (1986) Trypanosomiasis control and African rural development. 357 pp. London & New York, Longman.Google Scholar
Langely, P.A., Hall, M.J.R. & Felton, T. (1988) Determining the age of tsetse flies, Glossina spp. (Diptera: Glossinidae) an appraisal of the pteridine fluorescence technique. Bulletin of Entomological Research 78, 387395.CrossRefGoogle Scholar
Lehane, M.J., Chadwick, J., Hower, M.A., & Mail, T.S. (1986) Improvements in the pteridine method of determining age in adult Stomoxys calcitrans. Journal of Economic Entomology 79, 17141719.CrossRefGoogle Scholar
Lehane, M.J. & Hargrove, J. (1988) Field experiments on a new method for determining age in tsetse flies (Diptera: Glossinidae). Ecological Entomology 13, 319322.CrossRefGoogle Scholar
Lehane, M.J. & Mail, T.S. (1985) Determining the age of adult male and female Glossina morsitans morsitans using a new technique. Ecological Entomology 10, 219224.CrossRefGoogle Scholar
Mail, T.S., Chadwick, J. & Lehane, M.J. (1983) Determining the age of adults of Stomoxys calcitrans (L.) (Diptera: Muscidae). Bulletin of Entomological Research 73, 501525.CrossRefGoogle Scholar
Phelps, R.J. & Clark, G.P.Y. (1974) Seasonal elimination of some size classes in males of Glossina morsitans morsitans Westw. (Dipteria: Glossinidae). Bulletin of Entomological Research 64, 313324.CrossRefGoogle Scholar
Saunders, D.S. (1962) Age determination for female tsetse flies and the age composition of samples of Glossina pallidipes Aust, G. palpalis fuscipes Newst. and G. brevipalpis Newst. Bulletin of Entomological Research 53, 579595.CrossRefGoogle Scholar
Winston, P.W. & Bates, D.H. (1961) Saturated solutions for the control of humidity in biological research, Ecology 41, 232237.CrossRefGoogle Scholar