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Molecular markers to discriminate among four pest species of Helicoverpa (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  17 July 2008

G.T. Behere ,
W.T. Tay ,
D.A. Russell  and
P. Batterham
G.T. Behere*
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia
W.T. Tay
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia
D.A. Russell
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent, ME4 4TB, UK
P. Batterham
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia
*
*Author for correspondence Fax: +61 (3)93475352 E-mail: g.behere@pgrad.unimelb.edu.au
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Abstract

The four significant pest species in the Helicoverpa genus (H. armigera, H. assulta, H. punctigera and H. zea) are morphologically similar and can only be reliably distinguished through dissection of adult genitalia. Two partial regions of the mitochondrial DNA (mtDNA), the cytochrome oxidase subunit I (COI) and the cytochrome b (Cyt b) genes were amplified by PCR and digested with restriction endonucleases. The restriction patterns, generated by the endonucleases BstZ17I and HphI, demonstrated reliable differentiation of the four Helicoverpa pest species. This technique is fast, reliable and effective at distinguishing specimens irrespective of their life stages and offers support to conventional taxonomic differentiation based on morphological characters.

Keywords

molecular species diagnosisPCR-RFLPmitochondrial DNAHelicoverpa
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 98 , Issue 6 , December 2008 , pp. 599 - 603
Copyright
Copyright © 2008 Cambridge University Press

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