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Organophosphorus resistance in the sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae): a genetic study incorporating synergists

Published online by Cambridge University Press:  10 July 2009

P. B. Hughes
P. B. Hughes
Affiliation:
Biological and Chemical Research Institute, Department of Agriculture, New South Wales, P.M.B. 10, P.O., Rydalmere 2116, Australia
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Abstract

Pre-treatment of females of Lucilia cuprina (Wied.) with the synergist piperonyl butoxide increased the resistance factors of an organophosphorus-resistant strain Q to four organophosphates in comparison to the susceptible strain LS. However, pre-treatment with S,S,S-tributylphosphorothrithioate (TBPT) reduced the resistance factors of Q to three organophosphorus insecticides. As TBPT is an esterase inhibitor, it is suggested that esterases contribute to resistance in the Q strain. Resistance to diazinon in the Q strain is due to a single, partially-dominant gene on chromosome 4. By the use of the backcross and discriminating dose technique, resistance to parathion, fenitrothion, chlorfenvinphos and fenitro-oxon (dimethyl 3-methyl-4-nitrophenyl phosphate) (the latter two were synergised by piperonyl butoxide to obtain a genetically discriminating dose) was also shown to be controlled by a single, partially-dominant gene on chromosome 4. It is concluded that this gene confers resistance to organophosphorus compounds in L. cuprina, probably by providing an esterase with enhanced ability to degrade organophosphates.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 72 , Issue 4 , December 1982 , pp. 573 - 582
Copyright
Copyright © Cambridge University Press 1982

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References

Arnold, J. T. A. & Whitten, M. J. (1976). The genetic basis for organosphosphorus resistance in the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera, Calliphoridae).—Bull. ent. Res. 66, 561–568.CrossRefGoogle Scholar
Apperson, C. S. & Georghiou, G. P. (1975). Mechanisms of resistance to organophosphorus insecticides in Culex tarsalis.—J. econ. Ent. 68, 153–157.CrossRefGoogle ScholarPubMed
Attia, F. I., Shanahan, G. J. & Hughes, P. B. (1979). Synergism of diazinon against susceptible and organophosphorus resistant Lucilia cuprina (Wiedemann).—J. Aust. entomol. Soc. 18, 141–142.CrossRefGoogle Scholar
Busvine, J. R. (1981). Joint action of mixtures and drugs or pesticides.—Chemy Ind. no.15, 518–521.Google Scholar
Foster, G. G., Whitten, M. J., Konovalov, C., Arnold, J. T. A. & Maffi, G. (1981). Autosomal genetic maps of the Australian sheep blowfly, Lucilia cuprina dorsalis R.-D. (Diptera: Calliphoridae), and possible correlations with the linkage maps of Musca domestica (L.) and Drosophila melanogaster (Mg.).—Genet. Res. 37, 55–69.CrossRefGoogle Scholar
Hughes, P. B. (1978). Insecticide resistance in field populations of the sheep blowfly Lucilia cuprina (Wiedemann) in 1977.—Aust. J. exp. Agric. Anim. Husb. 18, 844–846.CrossRefGoogle Scholar
Hughes, P. B. (1981). Spectrum of cross-resistance to insecticides in field samples of the primary sheep blowfly, Lucilia cuprina.—Int. J. Parasitol. 11, 475–479.CrossRefGoogle Scholar
Lewis, J. B. & Lord, K. A. (1969). Metabolism of some organophosphorus insecticides by strains of housefly.—Proc. 5th Br. Insectic. Fungic. Conf. 2, 465–471.Google Scholar
McKenzie, J. A., Dearn, J. M. & Whitten, M. J. (1980). Genetic basis of resistance to diazinon in Victorian populations of the Australian sheep blowfly, Lucilia cuprina.—Aust. J. biol. Sci. 33, 85–95.CrossRefGoogle ScholarPubMed
Nakatsugawa, T. & Dahm, P. (1967). Microsomal metabolism of parathion.—Biochem. Pharmac. 16, 25–38.CrossRefGoogle Scholar
Nakatsugawa, T. & Morelli, M. A. (1976). Microsomal oxidation and insecticide metabolism. —pp. 61–114in Wilkinson, C. F. (Ed.). Insecticide biochemistry and physiology.–768 pp. New York, Plenum.CrossRefGoogle Scholar
Oppenoorth, F. J. (1971). Resistance in insects: the role of metabolism and the possible use of synergists.—Bull. Wld Hlth Org. 44, 195–202.Google ScholarPubMed
Plapp, F. W. Jr & Tong, H. H. C. (1966). Synergism of malathion and parathion against resistant insects: phosphorus esters with synergistic properties.—J. econ. Ent. 59, 11–15.CrossRefGoogle ScholarPubMed
Roxburgh, N. A. & Shanahan, G. J. (1973). A method for the detection and measurement of insecticide resistance in larvae of Lucilia cuprina (Wied.) (Dipt., Calliphoridae).—Bull. ent. Res. 63, 99–102.CrossRefGoogle Scholar
Sawicki, R. M. & Keiding, J. (1981). Factors affecting the sequential acquisition by Danish houseflies (Musca domestica L.) of resistance to organophosphorus insecticides.—Pestic. Sci. 12, 587–591.CrossRefGoogle Scholar
Schuntner, C. A. & Roulston, W. J. (1968). A resistance mechanism in organophosphorus-resistant strains of sheep blowfly (Lucilia cuprina).—Aust. J. biol. Sci. 21, 173–176.CrossRefGoogle ScholarPubMed
Shanahan, G. J. (1979). Genetics of diazinon resistance in larvae of Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae).—Bull. ent. Res. 69, 225–228.CrossRefGoogle Scholar
Shanahan, G. J. & Roxburgh, N. A. (1974). Effect of diazinon selection upon two organophosphorus-resistant field strains of Lucilia cuprina (Wied.) (Diptera, Calliphoridae).—Bull. ent. Res. 63, 567–571.CrossRefGoogle Scholar
Whitten, M. J., Foster, G. G., Arnold, J. T. & Konowalow, C. (1975). The Australian sheep blowfly, Lucilia cuprina.—pp. 401–418in King, R. C. (Ed.). Handbook of genetics. Volume 3.–874 pp. London, Plenum.Google Scholar
Wilkinson, C. F. (1971). Insecticide synergists and their mode of action.—Proc. 2nd Int. IUPAC Congr. Pestic. Chem. 2, 117–159.Google Scholar
Yang, R. S. H., Hodgson, E. & Dauterman, W. C. (1971). Metabolism in vitro of diazinon and diazoxon in susceptible and resistant houseflies.—J. agric. Fd Chem. 19, 14–19.CrossRefGoogle Scholar