Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-15T05:52:57.224Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA-based identification of South Korean Megaloptera larvae with taxonomic notes

Published online by Cambridge University Press:  26 June 2015

Sang Woo Jung ,
Tatyana S. Vshivkova  and
Yeon Jae Bae
Sang Woo Jung
Affiliation:
Department of Life Science, Graduate School, Korea University, Seoul 136-713, South Korea
Tatyana S. Vshivkova
Affiliation:
Institute of Biology and Soil Science, Russian Academy of Sciences, Far East Branch, Vladivostok 690022, Russia
Yeon Jae Bae*
Affiliation:
Division of Environmental Science and Ecological Engineering, Korea University, Seoul 136-713, South Korea
*
1 Corresponding author (e-mail: yjbae@korea.ac.kr).
Get access Rights & Permissions [Opens in a new window]

Abstract

Mitochondrial DNA (mtDNA) sequences, which serve as DNA barcodes, have been used to associate immature and adult stages of insects and to delineate species. The partial mitochondrial cytochrome c oxidase subunit I (COI) gene sequences for South Korean Megaloptera (all known in the adult stage) were tested to identify undetermined larvae as a rapid and effective method from 31 specimens (16 adults and 15 larvae). The COI gene sequences distinguished all six known Megaloptera species, with a low genetic distance between larvae and adults (0.50±0.21%). Based on the COI gene sequences, we associated five types of larvae with known adults including the following four species of newly described larval stages: Sialis annae Vshivkova, 1979 (Megaloptera: Sialidae); Neochauliodes formosanus (Okamoto, 1910) (Megaloptera: Corydalidae); Parachauliodes asahinai Liu et al., 2008 (Megaloptera: Corydalidae); and Protohermes xanthodes Navás, 1913 (Megaloptera: Corydalidae). The known larval stage of Sialis koreana Jung and Bae, 2012 (Megaloptera: Sialidae) was confirmed, and the morphological variation in the male genitalia of Sialis longidens Klingstedt, 1932 (Megaloptera: Sialidae) is discussed. A larval key to the six South Korean species of Megaloptera is provided.

Type
Systematics & Morphology
Information
The Canadian Entomologist , Volume 148 , Issue 2 , April 2016 , pp. 123 - 139
Copyright
© Entomological Society of Canada 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Subject editor: Christopher Buddle

References

Aspöck, U., Haring, E., and Aspöck, H. 2012. The phylogeny of the Neuropterida: long lasting and current controversies and challenges (Insecta: Endopterygota). Arthropod Systematics & Phylogeny, 70: 119–129.Google Scholar
Beutel, R.G., Friedrich, F., Hörnschemeyer, T., Pohl, H., Hünefeld, F., Beckmann, F., et al. 2010. Morphological and molecular evidence converge upon a robust phylogeny of the megadiverse Holometabola. Cladistics, 26: 1–15.Google Scholar
Bowles, D.E. and Mathis, M.L. 1992. Variation in the terminalia of Neohermes concolor with a key to males of Neohermes in eastern North America (Megaloptera: Corydalidae: Chauliodinae). Insecta Mundi, 6: 145–149.Google Scholar
Cameron, S.L., Sullivan, J., Song, H., Miller, K.B., and Whiting, M.F. 2009. A mitochondrial genome phylogeny of the Neuropterida (lace-wings, alderflies and snakeflies) and their relationship to the other holometabolous insect orders. Zoologica Scripta, 38: 575–590.CrossRefGoogle Scholar
Contreras-Ramos, A. and Harris, S.C. 1998. The immature stages of Platyneuromus (Corydalidae), with a key to the genera of larval Megaloptera of Mexico. Journal of the North American Benthological Society, 17: 489–517.Google Scholar
Elliott, J.M. 1996. British freshwater Megaloptera and Neuroptera: a key with ecological notes. Freshwater Biological Association Scientific Publication, 54: 1–69.Google Scholar
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39: 783–791.CrossRefGoogle ScholarPubMed
Flint, O.S., Evans, E.D., and Neunzig, H.H. 2008. Megaloptera and aquatic Neuroptera. In An introduction to the aquatic insects of North America. 4th edition. Edited by R.W. Merritt, K.W. Cummins, and M.B. Berg. Kendall/Hunt Publishing Company, Dubuque, Iowa, United States of America. Pp. 425–438.Google Scholar
Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299.Google Scholar
Hayashi, F. 2005. Megaloptera. In Aquatic insects of Japan: manual with keys and illustrations. Edited by R. Kawai and K. Tanida. Tokai University Press, Kanagawa, Japan. Pp. 379–386.Google Scholar
Hayashi, F. and Suda, S. 1995. Sialidae (Megaloptera) of Japan. Aquatic Insects, 17: 1–15.CrossRefGoogle Scholar
Hwang, J.M., Yoon, T.J., Suh, K.I., and Bae, Y.J. 2013. Molecular phylogeny evidence of altitudinal distribution and habitat adaptation in Korean Ephemera species (Ephemeroptera: Ephemeridae). Entomological Research, 43: 40–46.CrossRefGoogle Scholar
Jung, S.W. and Bae, Y.J. 2012. Taxonomic review of the Korean Megaloptera with description of Sialis koreana, new species. Entomological Research Bulletin, 28: 3–13.Google Scholar
Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111–120.Google Scholar
Klingstedt, H. 1932. Sialis longidens n. sp. Aus dem sudlichen Zentral-Sibirien. Memoranda Societatis pro Fauna et Flora Fennica, 8: 1–3.Google Scholar
Liu, X., Hayashi, F., and Yang, D. 2006. Systematics of the Protohermes xanthodes species-group in eastern Asia (Megaloptera: Corydalidae). Entomological Science, 9: 399–409.CrossRefGoogle Scholar
Liu, X., Hayashi, F., and Yang, D. 2007. Revision of the Neochauliodes sinensis species-group (Megaloptera: Corydalidae: Chauliodinae). Zootaxa, 1511: 29–54.CrossRefGoogle Scholar
Liu, X., Hayashi, F., and Yang, D. 2008. Systematics and biogeography of the fishfly genus Parachauliodes (Megaloptera: Corydalidae) endemic to the east Asian island. Systematic Entomology, 33: 560–578.Google Scholar
Liu, X., Hayashi, F., and Yang, D. 2014. Phylogeny of the family Sialidae (Insecta: Megaloptera) inferred from morphological data, with implications for generic classification and historical biogeography. Cladistics, 2014: 1–32.Google Scholar
Liu, X., Wang, Y., Shih, C., Ren, D., and Yang, D. 2012. Early evolution and historical biogeography of fishflies (Megaloptera: Chauliodinae): implications from a phylogeny combining fossil and extant taxa. Public Library of Science One, 7: 1–12.Google ScholarPubMed
Liu, X. and Yang, D. 2004. A revision of the genus Neoneuromus in China (Megaloptera: Corydalidae). Hydrobiologia, 517: 147–159.Google Scholar
Liu, X. and Yang, D. 2006. The genus Sialis Latreille, 1802 (Megaloptera: Sialidae) in Palaearctic China, with description of a new species. Entomologica Fennica, 17: 394–399.Google Scholar
Lunt, D.H., Zhang, D.X., Szymura, J.M., and Hewitt, G.M. 1996. The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Molecular Biology, 5: 153–165.Google Scholar
Meinander, M. 1996. Megaloptera Sialidae, alder flies. In Aquatic insects of North Europe: a taxonomic handbook. Edited by A.N. Nilsson. Apollo Books, Stenstrup, Denmark. Pp. 105–110.Google Scholar
Miller, K.B., Alarie, Y., and Whiting, M.F. 2007. Description of the larva of Notaticus fasciatus (Coleoptera: Dytiscidae) associated with adults using DNA sequence data. Annals of the Entomological Society of America, 100: 787–797.CrossRefGoogle Scholar
Navás, L. 1913. Neuroptera Asiatica. II series. Revue Russe d’Entomologie, 13: 424–430.Google Scholar
Nei, M. and Kumar, S. 2000. Molecular evolution and phylogenetics. Oxford University Press, New York, New York, United States of America.CrossRefGoogle Scholar
Neunzig, H.H. and Baker, J.R. 1991. Order Megaloptera. In Immature insects. Volume 2. Edited by F.W. Stehr. Kendall-Hunt, Dubuque, Iowa, United States of America. Pp. 112–122.Google Scholar
Okamoto, H. 1910. Die Sialiden Japan. Wiener Entomologische Zeitung, 29: 255–263.Google Scholar
Oswald, J.D. 2013. A catalogue of the species-group names of the extant and fossil Neuroptera, Megaloptera, Raphidioptera and Glosselytrodea (Insecta: Neuropterida) of the world [online]. Available from http://lacewing.tamu.edu/Species-Catalogue/index.html [accessed 22 December 2014].Google Scholar
Ruiter, D.E., Boyle, E.E., and Zhou, X. 2013. DNA barcoding facilitates associations and diagnoses for Trichoptera larvae of the Churchill (Manitoba, Canada) area. BMC Ecology, 13: 5.Google Scholar
Takeuchi, Y. and Hoshiba, H. 2012. The life histories of three species of Corydalidae (Megaloptera) from Japan. Aquatic Insects, 34: 55–63.Google Scholar
Takeuchi, Y. and Hoshiba, H. 2013. The life histories of two species of Sialidae (Megaloptera) from Japan. Aquatic Insects, 34: 205–216.Google Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA 6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30: 2725–2729.Google Scholar
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4876–4882.Google Scholar
Vshivkova, T.S. 1979. New data on the systematics and distribution of Megaloptera (Insecta) of the Far East. In Systematics and ecology of continental reservoirs of the Far East. Edited by V.Y. Levanidov, I.M. Levanidova, I.A. Chereshnev, and E.A. Makarchenko. Akademiya nauk SSSR, Vladivostok, Russia. Pp. 78–85.Google Scholar
Vshivkova, T.S. 1980. A new species of Sialis (Megaloptera: Sialidae) and preliminary data on the distribution of Far Eastern species. In Freshwater fauna of the Far East. Edited by V.Y. Levanidov. Akademiya nauk SSSR, Vladivostok, Russia. Pp. 75–79.Google Scholar
Vshivkova, T.S. 1995. Order. Megaloptera. In Key to the insects of Russian Far East. Volume IV. Edited by M.A. Kozlov and E.A. Makarchenko. Nauka, St. Petersburg, Russia. Pp. 9–34.Google Scholar
Vshivkova, T.S. 2001. Megaloptera. Dobsonflies and Alderflies. In Key to freshwater invertebrates of Russia and adjacent lands. Edited by S.J. Tsalolikhin. Nauka, St. Petersburg, Russia. Pp. 374–380. plates 191−196 (pp. 768−779).Google Scholar
Vshivkova, T.S. and Dubatolov, V.V. 2010. New records of Protohermes species (Megaloptera, Corydalidae, Corydalinae) from the south of the Russian Far East. Euroasian Entomological Journal, 9: 345–351.Google Scholar
Vshivkova, T.S. and Ito, T. 1993. A new record of Sialis longidens Klingstedt (Megaloptera, Sialidae) from Hokkaido, Northern Japan. Japanese Journal of Entomology, 61: 108.Google Scholar
Wang, Y., Liu, X., Winterton, L., and Yang, D. 2012. The first mitochondrial genome for the fishfly subfamily Chauliodinae and implications for the higher phylogeny of Megaloptera. Public Library of Science One, 7: 1–13.Google ScholarPubMed
Yang, D. and Yang, C.K. 1995. Megaloptera and aquatic Neuroptera. In Aquatic insects of China useful for monitoring water quality. Edited by L.F. Yang, L.X. Tian, and J.C. Morse. Hohai University Press, Nanjing, China. Pp. 250–259.Google Scholar
Yang, Q., Makarkin, V.N., Winterton, S.L., Khramov, A.V., and Ren, D. 2012. A remarkable new family of Jurassic insects (Neuroptera) with primitive wing venation and its phylogenetic position in Neuropterida. Public Library of Science One, 7: 1–38.Google Scholar
Yoon, I.B. 1988. Illustrated encyclopedia of fauna and flora of Korea. Aquatic Insects. Ministry of Education of Korea, Seoul, South Korea. Pp. 423–429.Google Scholar