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Intrathecal Synthesis of Autoantibodies to Myelin Basic Protein in MultipleSclerosis

Published online by Cambridge University Press:  18 September 2015

Ingrid Catz  and
Kenneth G. Warren
Ingrid Catz*
Affiliation:
Departments of Laboratory Medicine and Medicine (Neurology), University of Alberta Hospital, Edmonton, Alberta
Kenneth G. Warren
Affiliation:
Departments of Laboratory Medicine and Medicine (Neurology), University of Alberta Hospital, Edmonton, Alberta
*
Department of Laboratory Medicine, WMC-4B4-30, University of Alberta Hospital, Edmonton, Alberta, Canada T6G 2G3
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Abstract:

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A solid phase radioimmunoassay was used to detect anti-myelin basic protein (MBP) antibodies in the CSF and serum of multiple sclerosis (MS) patients and controls. CSF and serum samples were assayed prior to acid hydrolysis in order to detect free anti-MBP as well as after acid hydrolysis to measure the total (free and bound) amount of antibody. An anti-MBP index controlling for serum levels as well as the degree of breakdown of the blood brain barrier was used to estimate intrathecal synthesis of anti-MBP. MS patients with acute exacerbations or chronically progressive disease have significantly elevated levels of both free and total CSF anti-MBP. The anti-MBP index is also significantly increased in MS patients with both forms of active disease. Anti-MBP antibodies are intrathecally produced in MS patients with active disease.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 13 , Issue 1 , February 1986 , pp. 21 - 24
Copyright
Copyright © Canadian Neurological Sciences Federation 1986

References

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