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Influence of genetically modified rice containing codA gene on physiological metabolism and genetic horizontal transformation in fed rats

Published online by Cambridge University Press:  12 February 2007

Zhao Zhi-Hui ,
Yang Li-Tao ,
Ai Xiao-Jie ,
Zhang Da-Bing  and
Zou Si-Xiang
Zhao Zhi-Hui*
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China Shanghai Feed Inspection and Supervision Station, Shanghai 201106, China
Yang Li-Tao*
Affiliation:
Department of Biological Science and Technology, Nanjing University, Nanjing 210093, China Agro-biotech Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Ai Xiao-Jie
Affiliation:
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101, China
Zhang Da-Bing
Affiliation:
Agro-biotech Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Zou Si-Xiang*
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
*
*The first two authors did equivalent work in this study.
*The first two authors did equivalent work in this study.
**Corresponding author: E-mail: sixiangzou@yahoo.com.cn
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Abstract

To analyse the influence of genetically modified (GM) rice (Oryza sativa) containing the exogenous choline oxidase (codA) gene on physiological metabolism and genetic horizontal transformation in the fed rats, the animals were divided randomly into two groups. One group was fed with the feedstuff containing 30% of transgenic rice. The remaining (control) group was fed with the feedstuff containing 30% of non-transgenic rice. After 30 days, growth, physiological metabolism parameters such as growth hormone (GH), insulin, glucagon, 3,3′,5-triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), and the genetic horizontal transformation were analysed. Results indicated that the weight of all rats from the two groups was similar (P>0.05). Also, there was no significant difference in the level of GH, insulin, glucagon, T4, T3 and TSH between the treated and untreated rats. The serum urea nitrogen (SUN), glucose, the glutamate–pyruvate transaminase (GTP), as well as the total cholesterol, of treated rats were almost identical to those of the control (P>0.05). Moreover, no target DNA sequence of rice endogenous sucrose phosphate synthase (SPS) gene or exogenous choline oxidase (codA) gene was detected in organs (liver, muscle, pancreas, etc.) except the stomach, gastrointestinal and rectum contents, and no genetic horizontal transformation was observed in rats fed with transgenic rice.

Keywords

exogenous choline oxidase (codA)growthhormonesmetabolizePCRtransgenic rice
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 1 , April 2006 , pp. 65 - 70
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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