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Over-expression of GbRac1 gene in transgenic tobacco enhances disease resistance to Alternaria alternata in vitro

Published online by Cambridge University Press:  13 June 2008

Li Wei-Min ,
Wang Zhi-Xing  and
Jia Shi-Rong
Li Wei-Min
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wang Zhi-Xing
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jia Shi-Rong*
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
*Corresponding author. Email: jiasr@public3.bta.net.cn
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Abstract

GbRac1 gene was cloned from Gossypium barbadense with degenerate primers and 3′-RACE. Northern blot analysis indicated that GbRac1 mRNA was expressed abundantly in G. barbadense seedlings inoculated with Verticillium dehliae compared with mock-inoculated plants. A plant constitutive expression vector pRac harbouring GbRac1 gene was constructed and leaf discs of tobacco (Nicotiana tabacum L. cv. NC89) were transformed with pRac by Agrobacterium-mediated transformation. Disease challenge test of detached leaves of the transgenic plants by inoculation with Alternaria alternata showed that resistance was enhanced dramatically compared with the non-transgenic plants. Results suggest that GbRac1 gene might have potential application in the genetic engineering of plants with enhanced disease resistance.

Keywords

Gossypium barbadenseGTP-binding protein Ractransgenic tobaccoAlternaria alternatadisease resistance
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 73 - 77
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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References

Bokoch, GM (1994) Regulation of the human neutrophil NADPH oxidase by the Rac GTP-binding proteins. Current Opinion in Cell Biology 6: 212–218.CrossRefGoogle ScholarPubMed
Horsch, RB, Fry, JE, Hoffman, NL, Eichholts, D, Rogers, SG and Fraley, RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231.CrossRefGoogle Scholar
Kawasaki, T, Henmi, K, Ono, E, Hatakeyama, S, Iwano, M, Satoh, H et al. , (1999) The small GTP-binding protein Rac is a regulator of cell death in plants. Proceedings of the National Academy of Sciences of the USA 96: 10922–10926.CrossRefGoogle ScholarPubMed
Ma, C, Jian, GL and Sun, WJ (1997) Present status, constraints and countermeasures in cotton breeding for Verticillium wilt resistance in China. Chinese Agricultural Science 30: 58–64.Google Scholar
Morel, JB and Dangl, JL (1997) The hypersensitive response and the induction of cell death in plants. Cell Death and Differentiation 4: 671–683.CrossRefGoogle ScholarPubMed
Ono, E, Wong, HL, Kawasaki, T, Hasegawa, M, Kodama, O and Shimamoto, K (2001) Essential role of the small GTPase Rac in disease resistance of rice. Proceedings of the National Academy of Sciences of the USA 98: 759–764.CrossRefGoogle ScholarPubMed
Park, J, Choi, HJ, Lee, S, Lee, T, Yang, Z and Lee, Y (2000) Rac-related GTP-binding protein in elicitor-induced reactive oxygen generation by suspension-cultured soybean cells. Plant Physiology 124: 725–732.CrossRefGoogle ScholarPubMed
Pennell, RI and Lamb, C (1997) Programmed cell death in plants. Plant Cell 9: 1157–1168.CrossRefGoogle ScholarPubMed
Potikha, TS, Collins, CC, Johnson, DI, Delmer, DP and Levine, A (1999) The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers. Plant Physiology 119: 849–858.CrossRefGoogle ScholarPubMed
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.Google Scholar
Vaux, DL and Korsmeyer, SJ (1999) Cell death in development. Cell 96: 245–254.CrossRefGoogle ScholarPubMed
Yang, ZB (2002) Small GTPase: versatile signaling switches in plants. Plant Cell 14(Suppl.): 375–388CrossRefGoogle ScholarPubMed