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Cloning and sequence analysis of complete cDNA of chitin deacetylase from Mucor racemosus

Published online by Cambridge University Press:  02 August 2007

Jiang Xia-Yun ,
Zou Shu-Ming  and
Zhou Pei-Gen
Jiang Xia-Yun
Affiliation:
College of Food Science and Technology, Shanghai Fisheries University, Shanghai 200090, China
Zou Shu-Ming*
Affiliation:
Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem, Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China
Zhou Pei-Gen
Affiliation:
College of Food Science and Technology, Shanghai Fisheries University, Shanghai 200090, China
*
*Corresponding author. E-mail: smzou@shfu.edu.cn
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Abstract

A complete chitin deacetylase (CDA) complementary DNA (cDNA) from Mucor racemosus was cloned and sequenced by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification cDNA end (RACE) with gene special conserved primers. The cDNA sequence was submitted to GenBank (DQ538514). The complete cDNA with full-length of 1506 bp contained a 67 bp 5′-untranslated region, an open reading frame of 1344 bp and 95 bp 3′-untranslated region including tailing site AATAAA. The gene encoded a sequence of 448 amino acid residues and consisted of core nucleotides encoding a polysaccharide deacetylase domain covering 32% of the entire sequence. The CDA gene shared sequence homology with those of several fungi. The corresponding homology of the deduced amino acid sequences varied from 21 to 69%. Phylogenetic analysis according to the deduced amino acid sequences matched the classical fungi taxonomy. The three-dimensional structure of this protein was predicted. The protein had a whole CDA functional domain and a polysaccharide deacetylase domain.

Keywords

RACEchitin deacetylaseMucor racemosusphylogenetic analysis
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 167 - 172
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(6): 981–985

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