Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-10T11:45:16.015Z Has data issue: false hasContentIssue false
  • English
  • Français

Diversity of endophytic diazotrophs isolated from Bambusa blumeana in Guangdong province

Published online by Cambridge University Press:  02 August 2007

How Wei ,
Peng Gui-Xiang ,
Xu Zhi-Jun ,
Chen Shi-Xian  and
Tan Zhi-Yuan
How Wei
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
Peng Gui-Xiang
Affiliation:
College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China
Xu Zhi-Jun
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
Chen Shi-Xian
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
Tan Zhi-Yuan*
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
*
*Corresponding author. E-mail: zytan@scau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Endophytic bacteria isolated from Guangdong bamboo (Bambusa blumeana) plants were obtained under aerobic and anaerobic cultural conditions. Using the acetylene reduction assay (ARA), 40 strains were identified as diazotrophs. Diazotrophic isolates were grouped into five clusters (I, II, III, IV and V) by SDS-PAGE whole-cell protein patterns. The analysis of full-length 16S rDNA sequences of the representative strains of each group indicated that the endophytic diazotrophs showed great diversity and belonged to the genera Azospirillum (α-subclass of proteobacteria), Escherichia and Pseudomonas (γ-subclass of proteobacteria), and Aquaspirillum (β-subclass of proteobacteria).

Keywords

endophytic diazotrophsBambusa blumeanamicrobial diversity
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 105 - 109
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(2): 290–294

References

Ausubel, FM, Brent, R, Kingston, RE, et al. (1987) Current Protocols in Molecular Biology. New York: John Wiley & Sons.Google Scholar
Eckert, B, Weber, OB, Kirchhof, G, Halbritter, A, Stoffels, M and Hartmann, A (2001) Azospirillum doebereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass Miscanthus. International Journal of Systematic and Evolutionary Microbiology 51: 17–26.CrossRefGoogle Scholar
Gu, XP and Wu, XL (1994) A study on associated nitrogen fixation of bamboo rhizosphere. Forest Research 7(6): 18–23.Google Scholar
Gu, XP and Wu, XL (1999) Effects of inoculating associated nitrogen-fixing bacteria to moso bamboo seedlings growth. Forest Research 12(1): 7–12.Google Scholar
Gu, XP, Wu, XL and Wang, YD (2001) Study on associated nitrogen fixation of several sympodial bamboo species. Forest Research 14(1): 28–34.Google Scholar
Hafeez, FY and Malik, KA (2000) Manual on Biofertilizer Technology. Pakistan: NIBGE.Google Scholar
Han, WW, Shen, SH and Jing, YX (2004) Proteomics in biological nitrogen fixation. Journal of Agricultural Biotechnology 12(4): 464–469.Google Scholar
Hurek, T, Egener, T and Reinhold-Hurek, B (1997) Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the β-subclass. Journal of Bacteriology 179: 4172–4178.CrossRefGoogle ScholarPubMed
James, EK and Olivares, FL (1998) Infection and colonization of sugarcane and other graminaceous plants by endophytic diazotrophs. Critical Reviews in Plant Sciences 17: 77–119.Google Scholar
Lauren, DB, Humberto, JO and Fabio, O (2003) Endophytic Herbaspirillum seropedicae expresses nif genes in gramineous plants. Microbiology Ecology 45: 39–47.Google Scholar
Peng, GX, Wang, HR, Zhang, GX, Hou, W, Yuan, QH and Tan, ZY (2005) Molecular study of endophytic nitrogen-fixing bacteria isolated from Melinis minutiflora. Journal of South China Agricultural University 26(4): 73–76.Google Scholar
Peng, GX, Wang, HR, Zhang, GX, Hou, W, Liu, Y, Wang, ET and Tan, ZY (2006) Azospirillum melinis sp. nov., a group of diazotrophs isolated from tropical molasses grass. International Journal of Systematic and Evolutionary Microbiology 56: 1263–1271.CrossRefGoogle Scholar
Reinhold-Hurek, B and Hurek, T (1998) Life in grasses: diazotrophic endophytes. Trends in Microbiology 6: 139–144.Google Scholar
Sundaresan, V and Ausubel, FM (1981) Nucleotide sequence of the gene coding for the nitrogenase iron protein from Klebsiella pneumoniae. Journal of Biological Chemistry 256(6): 2808–2812.CrossRefGoogle ScholarPubMed
Tan, ZY and Chen, WX (1998) SDS-PAGE of whole-cell protein of novel rhizobial groups and 16S rDNA sequencing of their representatives. Chinese Journal of Applied and Environmental Biology 4(1): 65–69.Google Scholar
Tan, ZY, Gyaneshwar, P, Ladha, JK and Reinhold-Hurek, B (2001a) Novel endophytes of rice form a taxonomically distinct subgroup of Serratia marcescens. Systematic and Evolutionary Microbiology 24: 245–251.Google Scholar
Tan, ZY, Hurek, T, Vinuesa, P, Muller, P, Ladha, JK and Reinhold-Hurek, B (2001b) Specific detection of Bradyrhizobium and Rhizobium strains colonizing rice (Oryza sativa) roots by 16S–23S ribosomal DNA intergenic spacer-targeted PCR. Applied and Environment Microbiology 67: 3655–3664.CrossRefGoogle ScholarPubMed
Wose, CR (1987) Bacterial evolution. Microbiology Reviews 51: 221–271.Google Scholar
Yan, AM and Chen, WX (1998) SDS-PAGE analysis of whole-cell protein and numerical taxonomy of rhizobia of some legumes growing in arid region. Chinese Journal of Applied and Environmental Biology 4(4): 354–359.Google Scholar
Yao, T, Zhang, DG and Hu, ZZ (2004) Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region. Acta Prataculturaesinica Sinica 13: 106–111.Google Scholar
Zehr, JP and McReynolds, LA (1989) Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii. Applied Environmental Microbiology 55: 2522–2526.CrossRefGoogle ScholarPubMed