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Genetic diversity analysis of diazotrophs in the rice rhizosphere

Published online by Cambridge University Press:  13 February 2008

Chen Bin ,
Zheng Si-Ping ,
Zhou Li-Juan ,
Lin Zhi-Min ,
Song Ya-Na  and
Zheng Wei-Wen
Chen Bin
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
Zheng Si-Ping
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
Zhou Li-Juan
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
Lin Zhi-Min
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
Song Ya-Na
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
Zheng Wei-Wen*
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
*
*Corresponding author. E-mail: bcfaas01@hotmail.com
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Summary

The genetic diversity of dinitrogen-fixing bacteria associated with rice (Oryza sativa) was assessed by a polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) approach on the nifH gene amplified directly from DNA extracted from washed rice roots and rhizospheric soil. Restriction digestion with the enzymes MnlI and HaeIII was performed to characterize 54 cloned nifH PCR products. RFLP profiles were clustered and analysed with the UPGMA program. Eight pairs of similar RFLP patterns (similarity>50%) and two pairs of homologous RFLP patterns (100% identity) were found from the washed roots and the rhizospheric soil, respectively. Three specific diazotrophic patterns were found from rhizospheric soil and rice roots. The analyses have revealed the presence of different nifH types, which appear to be significant components of the diazotrophic community in paddy fields, indicating that some of the diazotrophs may colonize the inside and the surface of the rice roots.

Keywords

nifHpolymerase chain reactionrestriction fragment length polymorphismricerhizospheric soil
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 3 , December 2007 , pp. 253 - 258
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 841–846

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