Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-10T08:53:58.025Z Has data issue: false hasContentIssue false
  • English
  • Français

Phylogenetic analysis of Cryptosporidium isolates in Henan

Published online by Cambridge University Press:  13 February 2008

Wang Jin-Chan ,
Qi Wei-Wei ,
Zhang Long-Xian ,
Ning Chang-Shen ,
Jian Fu-Chun ,
Zhao Jin-Feng  and
Wang Ming
Wang Jin-Chan
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China Pu-like Bio-engineering Co., Ltd, Luoyang 471000, China
Qi Wei-Wei
Affiliation:
College of Veterinary Medicine,China Agricultural University, Beijing 100094, China
Zhang Long-Xian*
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Ning Chang-Shen
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Jian Fu-Chun
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Zhao Jin-Feng
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Wang Ming*
Affiliation:
College of Veterinary Medicine,China Agricultural University, Beijing 100094, China
*
*Corresponding authors. E-mail: zhanglx8999@yahoo.com.cn or vedean@cau.edu.cn
*Corresponding authors. E-mail: zhanglx8999@yahoo.com.cn or vedean@cau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The functional mitochondrial protein alternative oxidase (AOX) gene was used as a marker to analyse the phylogenetic relationship between Cryptosporidium isolates. This gene was characterized, and the phylogentic tree was established from Cryptosporidium isolates and compared to those generated from 18S rRNA and heat-shock protein 70 (HSP70) gene sequences. The present trial aimed at finding out whether the AOX gene is suitable for phylogenetic analysis of the Cryptosporidium genus. The results revealed that the genus Cryptosporidium contained the phylogenetically distinct species C. parvum, C. hominis, C. suis and C. baileyi, which were consistent with the biological characterization and host specificity reported earlier. Cryptosporidium species formed two clades: one included C. hominis, C. suis, C. parvum cattle genotypes and C. parvum mouse genotype; and the other comprised C. meleagridis and C. baileyi isolates. Within C. parvum, both the mouse genotype and the pig genotype I (also known as C. suis) isolates differed from cattle and human (also known as C. hominis) genotypes, based on the aligned nucleotide sequences. The sequence identity of the AOX gene was higher between C. meleagridis and C. baileyi than between C. meleagridis and C. parvum. The phylogenetic trees showed that C. meleagridis was closer to C. baileyi than to C. parvum. This result was inconsistent with the phylogenetic analysis deduced from 18S rRNA and HSP70 gene sequences, respectively. The present results suggest that the AOX gene is not only equally suitable for the phylogenetic analysis of Cryptosporidium, but also provides an outstanding and new approach in determining Cryptosporidium heredity.

Keywords

Cryptosporidiumalternative oxidasephylogenetic relationship
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 3 , December 2007 , pp. 247 - 252
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this work.

First published in Journal of Agricultural Biotechnology 2007, 15(5): 793–798

References

Arrowood, M and Sterling, G (1987) Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic percoll gradients. Journal of Parasitology 73: 314–319.CrossRefGoogle ScholarPubMed
Bonnin, A, Fourmaux, M, Dubremetz, J, et al. (1996) Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction–restriction fragment length polymorphism analysis of a repetitive DNA sequence. FEMS Microbiology Letters 137: 207–211.CrossRefGoogle ScholarPubMed
Caccio, S, Homan, K, Van, D, et al. (1999) Genetic polymorphism at the beta-tubulin locus among human and animal isolates of Cryptosporidium parvum. FEMS Microbiology Letters 170: 173–179.CrossRefGoogle ScholarPubMed
Carraway, M, Tzipori, S and Widmer, G (1996) Identification of genetic heterogeneity in the Cryptosporidium parvum ribosomal repeat. Applied and Environmental Microbiology 62: 712–716.CrossRefGoogle ScholarPubMed
Fayer, R, Morgan, U and Upton, S (2000) Epidemiology of Cryptosporidium: transmission, detection and identification. International Journal of Parasitology 30: 1305–1322.CrossRefGoogle ScholarPubMed
Fayer, R, Santin, M and Xiao, L (2005) Cryptosporidium bovis n. sp. (Apicomplexa: Cryptosporididae) in cattle (Bos taurus). Journal of Parasitology 91: 624–629.CrossRefGoogle Scholar
Liu, C, Widmer, G, Tzipori, S, et al. (2004) Complete genome sequence of the apicomplexan, Cryptosporidium parvum. Science 304: 441–445.Google Scholar
Margarida, A, Xiao, L, Sulaiman, I, et al. (2003) Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. Journal of Clinical Microbiology 41: 2744–2747.Google Scholar
Morgan, U, Sargent, K, Deplazes, P, et al. (1998) Molecular characterization of Cryptosporidium from various hosts. Parasitology 117: 31–37.CrossRefGoogle ScholarPubMed
Morgan, U, Deplazes, P and Forbes, D (1999) Sequence and PCR-RFLP analysis of the internal transcribed spacers of the rDNA repeat unit in isolates of Cryptosporidium from different hosts. Parasitology 118: 49–58.CrossRefGoogle ScholarPubMed
Peng, M, Xiao, L, Freeman, A, et al. (1997) Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerging Infectious Disease 3: 563–573.CrossRefGoogle ScholarPubMed
Ryan, U, Monis, P, Enemark, H, et al. (2004) Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporididae) in pigs (Sus scrofa). Journal of Parasitology 90: 769–773.CrossRefGoogle Scholar
Schmidt, H, Strimmer, K, Vingron, M, et al. (2002) TREE-PUZZLE: maximum likelihood phylogenetic analysis using quarter and parallel computing. Bioinformatics 18: 502–504.CrossRefGoogle Scholar
Spano, F, Putignani, L and McLauchlin, J (1997) PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between Cryptosporidium wrairi and Cryptosporidium parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiolology Letters 150: 209–217.CrossRefGoogle ScholarPubMed
Spano, F, Putignani, L, Guida, S, et al. (1998) Cryptosporidium parvum: PCR-RFLP analysis of the TRAP-C1 (thrombospondin-related adhesive protein of Cryptosporidium-1) gene discriminates between two alleles differentially associated with parasite isolates of animal and human origin. Experimental Parasitology 90: 195–198.CrossRefGoogle ScholarPubMed
Sreter, T, Kovacs, G and da Silva, AJ (2000) Morphologic culture and molecular characterization of a Hungarian Cryptosporidium meleagridis. Journal of Parasitology 86: 1244–1249.CrossRefGoogle Scholar
Sulaiman, I, Morgan, U, Andrew-Thompson, R, et al. (2000) Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene. Applied and Environmental Microbiology 66: 2385–2391.CrossRefGoogle ScholarPubMed
Swofford, D (2002) Phylogenetic analysis using parsimony (PAUP), version 4.0. Champaign: Illinois Natural History Survey, pp. 1–144.Google Scholar
Thompson, J, Gibson, T, Plewniak, F, et al. (1997) The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis too. Nucleic Acids Research 24: 4876–4882.CrossRefGoogle Scholar
Tzipori, S and Ward, H (2002) Cryptosporidiosis: biology, pathogenesis and disease. Microbiology Infection 4: 1047–1058.CrossRefGoogle ScholarPubMed
Vasquez, J, Gooze, L, Kim, K, et al. (1996) Potential antifolate resistance determinants and genotypic variation in the bifunctional dihydrofolate reductase–thymidylate synthase gene from human and bovine isolates of Cryptospridium parvum. Molecular Biology of Parasitology 79: 153–165.CrossRefGoogle Scholar
Wang, Jin-chan, Jian, Fu-chun, Zhang, Long-xian, et al. (2007) Phylogenetic analysis of the Cryptosporidium based on 18S rRNA gene and HSP70 gene sequences. Acta Veterinaria et Zootechnica Sinica 38(9): 947–953.Google Scholar
Xiao, L, Morgan, U, Limor, J, et al. (2000) Identification of species and sources of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Applied and Environmental Microbiology 66: 5492–5498.CrossRefGoogle ScholarPubMed
Xiao, L, Limor, J, Bern, C, et al. (2001) Tracking Cryptosporidium parvum by sequence analysis of small double-stranded RNA. Emerging Infectious Diseases 7: 141–145.CrossRefGoogle ScholarPubMed
Xiao, L, Fayer, R, Ryan, U, et al. (2004) Cryptosporidium taxonomy: recent advances and implications for public health. Clinical Microbiology 17: 72–97.CrossRefGoogle ScholarPubMed
Xu, Ping, Widmer, G, Wang, Y, et al. (2004) The genome of Cryptosporidium hominis. Nature 431: 1107–1112.CrossRefGoogle ScholarPubMed