Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-28T17:50:55.720Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of four species of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from roe deer (Capreolus capreolus) in the Netherlands

Published online by Cambridge University Press:  15 May 2009

S. G. T. Rijpkema ,
R. G. Herbes ,
N. Verbeek-De Kruif  and
J. F. P. Schellekens
S. G. T. Rijpkema
Affiliation:
Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, PO Box 1 3720 BA Bilthoven, The Netherlands
R. G. Herbes
Affiliation:
Veterinary Health Inspectorate, Arnhem, The Netherlands
N. Verbeek-De Kruif
Affiliation:
Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, PO Box 1 3720 BA Bilthoven, The Netherlands
J. F. P. Schellekens
Affiliation:
Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, PO Box 1 3720 BA Bilthoven, The Netherlands
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Roe deer (Capreolus capreolus) were investigated for their value as sentinel animals for Lyme borreliosis in the Netherlands. Serum was obtained from 114 roe deer, and 513 Ixodes ricinus, predominantly females (72%), were obtained from 47 animals (41%). The polymerase chain reaction was used to detect DNA of Borrelia burgdorferi sensu lato in a total of 190 ticks, comprising 106 engorged ticks and 84 non-engorged ticks. Borrelia DNA was detected in 24 engorged ticks (23%) and 26 non-engorged ticks (31%). This difference was not significant (P = 0·25). Four species of B. burgdorferi sensu lato were identified in the ticks: B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii and group VS116. B. afzelii was most commonly found and present in 13 mixed infections, and in 28 single infections. Fifteen sera (13%) contained antibodies to Borrelia spp. Ticks are more appropriate sentinel animals for Lyme borreliosis than roe deer, an important host for I. ricinus. Although the viability of borrelia spirochaetes in engorged ticks collected from roe deer was not assessed, a bloodmeal taken from roe deer did not eliminate borrelia spirochaetes from the tick. The relevance of this finding for transovarial transmission of borrelia spirochaetes in ticks is discussed.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 3 , December 1996 , pp. 563 - 566
Copyright
Copyright © Cambridge University Press 1996

References

1.Burgdorfer, W, Barbour, AG, Hayes, SF, Benach, JL, Grunwalt, E, Davis, JP. Lyme disease: a tick-borne spirochetosis? Science 1982; 216: 1317–9.CrossRefGoogle ScholarPubMed
2.Baranton, G, Postic, D, Saint, Girons I et al. , Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461. Int J Syst Bacteriol 1992; 42: 378–83.CrossRefGoogle ScholarPubMed
3.Canica, MM, Nato, F, du Merle, L, Mazie, JC, Baranton, G, Postic, D. Monoclonal antibody for the identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis. Scand J Infect Dis 1993; 25: 441–8.CrossRefGoogle ScholarPubMed
4.Postic, D, Assous, M, Grimont, PAD, Baranton, G. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment polymorphism of rrf (5S)- rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol 1994; 44: 743–52.CrossRefGoogle ScholarPubMed
5.Van Dam, AP, Kuiper, H, Vos, K et al. , Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin Infect Dis 1993; 17: 707–17.CrossRefGoogle ScholarPubMed
6.Nohlmans, LMKE, De Boer, R, Van Den Boogaard, AEJM, Van Boven, CP. Genotypic and phenotypic analysis of Borrelia burgdorferi isolates from The Netherlands. J Clin Microbiol 1995; 33: 119–25.CrossRefGoogle ScholarPubMed
7.Hovmark, A, Jaenson, TGT, Asbrink, E, Forsman, A, Jaensson, E. First isolation of Borrelia burgdorferi from rodents collected in Northern Europe. APMIS 1988; 96: 917–20.CrossRefGoogle ScholarPubMed
8.De Boer, R, Hovius, KE, Nohlmans, MKE, Gray, JS. The woodmouse (Apodemus sylvaticus) a reservoir of tick transmitted spirochetes (Borrelia burgdorferi) in The Netherlands. Zbl Bakt Hyg A 1993; 279: 404–16.CrossRefGoogle ScholarPubMed
9.Lastavica, CC, Wilson, ML, Berardi, VP, Spielman, A, Deblinger, RD. Rapid emergence of a focal epidemic of Lyme disease in coastal Massachusetts. N Engl J Med 1989; 320: 134–7.CrossRefGoogle ScholarPubMed
10.Rijpkema, S, Nieuwenhuijs, J, Franssen, FFJ, Jongejan, F. Infection rates of Borrelia burgdorferi in different instars of Ixodes ricinus ticks from the Dutch North sea island of Ameland. Exp Appl Acarol 1994; 18: 531–42.CrossRefGoogle ScholarPubMed
11.Halouzka, J, Juricova, Z, Mátlová, L, Hubáleck, Z. Borreliae in larval Ixodes ricinus ticks. Med Vet Entomol 1995; 9: 205–6.CrossRefGoogle ScholarPubMed
12.Kurtenbach, K, Kampen, H, Dizij, A et al. , Infestation of rodents with larval Ixodes ricinus (Acari: Ixodidae) is an important factor in the transmission cycle of Borrelia burgdorferi s.l. in German woodlands. J Med Entomol 1995; 32: 807–17.CrossRefGoogle ScholarPubMed
13.Randolph, SE, Craine, N. General framework of comparative quantitative studies on transmission of tick-borne diseases using Lyme borreliosis in Europe as an example. J Med Entomol 1995; 32: 765–77.CrossRefGoogle ScholarPubMed
14.Jaenson, TGT, Taelleklint, L. Incompetence of roe deer as reservoirs of the Lyme borreliosis spirochete. J Med Entomol 1992; 29: 813–7.CrossRefGoogle ScholarPubMed
15.Kimura, K, Isogai, E, Isogai, H et al. , Detection of Lyme disease spirochetes in the skin of naturally infected wild Sika deer (Cervus nippon yesoensis) by PCR. Appl Environm Microbiol 1995; 61: 1641–2.CrossRefGoogle ScholarPubMed
16.Lacombe, E, Rand, PW, Smith, RP Jr. Disparity of Borrelia burgdorferi infection rates of adult Ixodes dammini on deer and vegetation. J Infect Dis 1993; 167: 1236–8.CrossRefGoogle ScholarPubMed
17.Matuschka, FR, Heiler, M, Eiffert, H, Fischer, P, Lotter, H, Spielman, A. Diversionary role of hoofed game in the transmission of Lyme disease spirochetes. Am J Trop Med Hyg 1993; 48: 693–9.CrossRefGoogle ScholarPubMed
18.Gill, JS, McLean, RG, Neitzel, DF, Johnson, RC. Serologic analysis of white-tailed deer sera for antibodies to Borrelia burgdorferi by enzyme-linked immunosorbent assay and Western immunoblotting. J Clin Microbiol 1993; 31: 318–22.CrossRefGoogle ScholarPubMed
19.Webster, P, Frandsen, F. Prevalence of antibodies to Borrelia burgdorferi in Danish deer. APMIS 1994; 102: 287–90.CrossRefGoogle ScholarPubMed
20.Magnarelli, LA, Denicola, A, Stafford, KC III, Anderson, JF. Borrelia burgdorferi in an urban environment: white-tailed deer with infected ticks and antibodies. J Clin Microbiol 1995; 33: 541–4.CrossRefGoogle Scholar
21.Rijpkema, S, Golubic, D, Molkenboer, M, Verbeek-De Kruif, N, Schellekens, J. Identification of four species of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in a Lyme borreliosis endemic region in northern Croatia. Exp Appl Acarol 1996; 20: 23–30.CrossRefGoogle Scholar
22.Rijpkema, SGT, Molkenboer, MJCH, Schouls, LM, Jongejan, F, Schellekens, JFP. Simultaneous detection and genotyping of three species of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J Clin Microbiol 1995; 33: 3091–5.CrossRefGoogle ScholarPubMed
23.Wesson, DM, McLain, DK, Oliver, JH, Piesman, J, Collins, FH. Investigation of the validity of species status of Ixodes dammini (Acari: Ixodidae) using rDNA. Proc Natl Acad Sci USA 1993; 90: 10221–5.CrossRefGoogle ScholarPubMed
24.Rijpkema, S, Groen, J, Molkenboer, M, Herbrink, P, Osterhaus, A, Schellekens, J. Serum antibodies to the flagellum of Borrelia burgdorferi measured with an inhibition enzyme-linked immunosorbent assay are diagnostic for Lyme borreliosis. Serodiagn Immunother Infect Dis 1994; 6: 61–7.CrossRefGoogle Scholar
25.Gray, JS, Hayden, TJ, Casey, S, Kirstein, F, Rijpkema, S, Curtin, S. A Lyme disease serosurvey of deer in Irish national parks. Biol Environm 1996; 96B: 27–32.Google Scholar
26.Schwan, TG, Piesman, J, Golde, WT, Dolan, MC, Rosa, PA. Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Proc Natl Acad Sci USA 1995; 92: 2909–13.CrossRefGoogle ScholarPubMed