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The European Sero-Epidemiology Network: standardizing the enzyme immunoassay results for measles, mumps and rubella

Published online by Cambridge University Press:  06 November 2000

N. ANDREWS ,
R. G. PEBODY ,
G. BERBERS ,
C. BLONDEAU ,
P. CROVARI ,
I. DAVIDKIN ,
P. FARRINGTON ,
F. FIEVET-GROYNE ,
G. GABUTTI  and
E. GERIKE
...Show all authors
N. ANDREWS
Affiliation:
PHLS, Communicable Disease Surveillance Centre, 61 Colindale Avenue, London NW9 5EQ, UK
R. G. PEBODY
Affiliation:
PHLS, Communicable Disease Surveillance Centre, 61 Colindale Avenue, London NW9 5EQ, UK
G. BERBERS
Affiliation:
National Institute of Public Health and the Environment, Bilthoven, The Netherlands
C. BLONDEAU
Affiliation:
Pasteur-Merieux Connaught, Paris, France
P. CROVARI
Affiliation:
Dept. of Health Sciences-Hygiene and Preventive Medicine Section, Faculty of Medicine, University of Genoa, Italy
I. DAVIDKIN
Affiliation:
National Public Health Institute, Helsinki, Finland
P. FARRINGTON
Affiliation:
Department of Statistics, the Open University, Milton Keynes, UK
F. FIEVET-GROYNE
Affiliation:
Pasteur-Merieux Connaught, Paris, France
G. GABUTTI
Affiliation:
Dept. of Health Sciences-Hygiene and Preventive Medicine Section, Faculty of Medicine, University of Genoa, Italy Lab of Hygiene, Dept of Biology, Faculty of Science, University of Lecce, Italy
E. GERIKE
Affiliation:
Robert Koch Institute, Berlin, Germany
C. GIORDANO
Affiliation:
Dept. of Health Sciences-Hygiene and Preventive Medicine Section, Faculty of Medicine, University of Genoa, Italy
L. HESKETH
Affiliation:
Preston PHL, Preston, UK
T. MARZEC
Affiliation:
National Institute of Public Health and the Environment, Bilthoven, The Netherlands
P. MORGAN-CAPNER
Affiliation:
Preston PHL, Preston, UK
K. OSBORNE
Affiliation:
PHLS, Communicable Disease Surveillance Centre, 61 Colindale Avenue, London NW9 5EQ, UK
A. M. PLEISNER
Affiliation:
Statens Serum Institut Copenhagen, Denmark
M. RAUX
Affiliation:
Pasteur-Merieux Connaught, Paris, France
A. TISCHER
Affiliation:
Robert Koch Institute, Berlin, Germany
U. RUDEN
Affiliation:
Swedish Institute of Infectious Disease Control, Stockholm, Sweden
M. VALLE
Affiliation:
National Public Health Institute, Helsinki, Finland
E. MILLER
Affiliation:
PHLS, Communicable Disease Surveillance Centre, 61 Colindale Avenue, London NW9 5EQ, UK
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Abstract

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The ESEN (European Sero-Epidemiology Network) project was established to harmonize the seroepidemiology of five vaccine preventable infections including measles, mumps and rubella in eight European countries. This involved achieving comparability both in the assay results from testing in different centres and also sampling methodology. Standardization of enzyme immunoassay results was achieved through the development of common panels of sera by designated reference centres. The panels were tested at the reference laboratory and then distributed to each participating laboratory for testing using their routine methods. Standardization equations were calculated by regressing the quantitative results against those of the reference laboratory. Our study found large differences in unitage between participants, despite all using an EIA method standardized against an international or local standard. Moreover, our methodology adjusted for this difference. These standardization equations will be used to convert the results of main serosurvey testing into the reference country unitage to ensure inter-country comparability.

Type
Research Article
Information
Epidemiology & Infection , Volume 125 , Issue 1 , August 2000 , pp. 127 - 143
Copyright
© 2000 Cambridge University Press