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Identification of Helicobacter pylori DNA in Iranian patients with gallstones

Published online by Cambridge University Press:  16 November 2004

SH. FARSHAD ,
A. ALBORZI ,
S. A. MALEK HOSSEINI ,
B. OBOODI ,
M. RASOULI ,
A. JAPONI  and
J. NASIRI
SH. FARSHAD
Affiliation:
Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
A. ALBORZI
Affiliation:
Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
S. A. MALEK HOSSEINI
Affiliation:
Department of Surgery, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
B. OBOODI
Affiliation:
Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
M. RASOULI
Affiliation:
Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
A. JAPONI
Affiliation:
Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
J. NASIRI
Affiliation:
Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
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Abstract

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In order to identify Helicobacter in gallstones of Iranian patients with biliary disease, gallstone and bile samples from 33 patients were subjected to rapid urease test, culture and Multiplex PCR using primers based on 16s rRNA and isocitrate dehydrogenase genes for the identification of Helicobacter genus and H. pylori respectively. This PCR was also done on bile samples from 40 autopsied gallbladders with normal pathology (control group). In 18·1% of stone and 12·1% of bile samples, H. pylori DNA was detected using PCR. Rapid urease and culture tests were negative for all samples. The PCR was negative in the control group. In conclusion, H. pylori DNA was detected in stone samples of Iranian patients with gallstones but we are not sure of their viability. To clarify the clinical role of Helicobacter in gallbladder diseases, studies using accurate tests on larger patient and control groups are needed to ascertain whether this microorganism is an innocent bystander or active participant in gallstone formation.

Type
Short Report
Information
Epidemiology & Infection , Volume 132 , Issue 6 , December 2004 , pp. 1185 - 1189
Copyright
© 2004 Cambridge University Press