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IgA antibody response in acute rubella determined by solid-phase radioimmunoassay

Published online by Cambridge University Press:  15 May 2009

P. Halonen ,
O. Meurman ,
Marja-Terttu Matikainen ,
E. Torfason  and
H. Bennich
P. Halonen
Affiliation:
Department of Virology, University of Turku, 20520 Turku 52Finland
O. Meurman
Affiliation:
Department of Virology, University of Turku, 20520 Turku 52Finland
Marja-Terttu Matikainen
Affiliation:
Department of Virology, University of Turku, 20520 Turku 52Finland
E. Torfason
Affiliation:
Biomedical Center, University of Uppsala, Uppsala, Sweden
H. Bennich
Affiliation:
Biomedical Center, University of Uppsala, Uppsala, Sweden
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A solid-phase radioimmunoassay (RIA) for detecting rubella virus IgA serum antibodies was developed. Purified rubella virus grown in roller cultures of Vero cells was adsorbed onto polystyrene beads. The coated beads were then incubated with dilutions of serum, and rubella IgA antibodies which attached to the virus antigen on the solid-phase were subsequently detected with 125I-labelled anti-human-alpha antibodies. The specificity of the iodinated anti-human immunoglobulins was confirmed by RIA analysis of fractions obtained by chromatography of an early convalescent serum on an agarose column. A complete separation of IgM, IgA, and IgG was observed.

A total of 144 serial serum specimens from 31 adult patients with an acute rubella infection were tested for rubella IgA antibodies, and the results were compared with the RIA IgG and IgM titres reported earlier from the same specimens. The RIA IgA response was detected in each of the 31 patients and the IgA antibodies appeared almost simultaneously with the IgG and IgM antibodies. The maximum titres, which were lower than the IgG and IgM titres, were reached in about 1 week after the onset of rash. In 6 patients out of 31 the IgA antibody response was transient and persisted approximately two months, while in the remaining 25 patients the IgA antibodies persisted throughout the study period of more than 5 months. The results obtained indicate that the presence of rubella IgA antibodies in serum is not an indication for a recent rubella infection.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 1 , August 1979 , pp. 69 - 75
Copyright
Copyright © Cambridge University Press 1979

References

Al-Nakib, W., Best, J. M. & Banatvala, J. E. (1975). Rubella-specific serum and nasopharyngeal immunoglobulin responses following naturally acquired and vaccine-induced infection. Prolonged persistence of virus-specific IgM. Lancet i, 182.Google Scholar
Ankerst, J., Christensen, P., KjelléN, L. & Kronvall, G. (1974). A routine diagnostic test for IgA and IgM antibodies to rubella virus: absorption of IgG with Staphylococcus aureus. Journal of Infectious Diseases 130, 268.CrossRefGoogle Scholar
Bürgin-Wolff, A., Hernandez, R. & Just, M. (1971). Separation of rubella IgM, IgA and IgG antibodies by gel filtration on agarose. Lancet ii, 1278.Google Scholar
Cradock-Watson, J. E., Bourne, M. S. & Vandervelde, E. M. (1972). IgG, IgA, and IgM responses in acute rubella determined by the immunofluorescent technique. Journal of Hygiene 70, 473.CrossRefGoogle ScholarPubMed
Halonen, P., Bennich, H., Torfason, E., Karlsson, T., Ziola, B., Hjelteson, E., Matikainen, M.-T. & Wesslen, T. (1979). Solid-phase radioimmunoassay of respiratory syncytial virus and adenovirus immunoglobulin A serum antibodies. Journal of Clinical Microbiology (in the press).CrossRefGoogle Scholar
Hornsleth, A., LeerhØY, J., Grauballe, P. & Spanggaard, H. (1975). Persistence of rubella virus-specific immunoglobulin M and immunoglobulin A antibodies: Investigation of successive serum samples with lowered immunoglobulin G concentration. Infection and Immunity 11, 804.CrossRefGoogle Scholar
Hunter, W. M. & Greenwood, F. C. (1962). Preparation of iodine-131 labelled human growth hormone with high specific activity. Nature 194, 495.CrossRefGoogle ScholarPubMed
Kalimo, K. O. K., Meurman, O. H., Halonen, P. E., Ziola, B. R., Viljanen, M. K., Granfors, K. & Toivanen, P. (1976). Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies. Journal of Clinical Microbiology 4, 117.Google Scholar
Meurman, O. H. (1978). Antibody responses in patients with rubella infection determined by passive hemagglutination, hemagglutination inhibition, complement fixation, and solid-phase radioimmunoassay tests. Infection and Immunity 19, 369Google Scholar
Meurman, O. H., Viljanen, M. K. & Granfors, K. (1977). Solid-phase radioimmunoassay of rubella virus immunoglobulin M antibodies: Comparison with sucrose density gradient centrifugation test. Journal of Clinical Microbiology 5, 257.CrossRefGoogle ScholarPubMed
Meurman, O. H. & Ziola, B. R. (1978). IgM-class rheumatoid factor interference in the solid-phase radioimmunoassay of rubella-specific IgM antibodies. Journal of Clinical Pathology 31, 483.CrossRefGoogle ScholarPubMed
Ogra, P. L., Kerr-grant, D., Umana, G., Dzierba, J. & Weintraub, D. (1971). Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus. New England Journal of Medicine 285, 1333.Google Scholar
Pattison, J. R. & Mace, J. E. (1975). Elution patterns of rubella IgM, IgA, and IgG anti-bodies from a dextran and an agarose gel. Journal of Clinical Pathology 28, 670.CrossRefGoogle Scholar
Roggendorf, M., Schneweis, K. E. & Wolff, M. H. (1976). Zum Nachweis Röteln-spezifischer IgM im Hämagglutinationshemmungstest. Vergleichende Untersuchungen mit der Absorption von IgG durch Protein A-haltige Staphylokokken und der Dichtegradienten-Ultrazentrifugation. Zentralblatt für Bakteriologie, Parasitenheilkunde, Infektionskrankheiten und Hygiene A 235, 363.Google Scholar
Schmitz, H., Shimizu, H., Kampa, D. & Doerr, H. W. (1975). Rapid method to detect rubella immunoglobulin M and immunoglobulin A antibodies. Journal of Clinical Microbiology 1, 132.Google Scholar