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Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus

Published online by Cambridge University Press:  25 June 2003

T. IKEGAMI ,
M. SAIJO ,
M. NIIKURA ,
M. E. MIRANDA ,
A. B. CALAOR ,
M. HERNANDEZ ,
D. L. MANALO ,
I. KURANE ,
Y. YOSHIKAWA  and
S. MORIKAWA
T. IKEGAMI
Affiliation:
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
M. SAIJO
Affiliation:
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan
M. NIIKURA
Affiliation:
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan
M. E. MIRANDA
Affiliation:
Veterinary Research Department, Research Institute for Tropical Medicine, Muntinlupa, Philippines
A. B. CALAOR
Affiliation:
Veterinary Research Department, Research Institute for Tropical Medicine, Muntinlupa, Philippines
M. HERNANDEZ
Affiliation:
Veterinary Research Department, Research Institute for Tropical Medicine, Muntinlupa, Philippines
D. L. MANALO
Affiliation:
Veterinary Research Department, Research Institute for Tropical Medicine, Muntinlupa, Philippines
I. KURANE
Affiliation:
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan
Y. YOSHIKAWA
Affiliation:
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
S. MORIKAWA
Affiliation:
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan
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Abstract

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We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360–739), and RΔ6 (aa 451–551) and/or RΔ8 (aa 631–739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

Type
Research Article
Information
Epidemiology & Infection , Volume 130 , Issue 3 , June 2003 , pp. 533 - 539
Copyright
© 2003 Cambridge University Press