Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-28T01:27:55.323Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular epidemiology of shigella infections in Israel

Published online by Cambridge University Press:  15 May 2009

M. Yavzori ,
D. Cohen  and
H. Bercovier
M. Yavzori
Affiliation:
Israel Defense Force, Medical Corps, Military Post 02149, Israel
D. Cohen
Affiliation:
Israel Defense Force, Medical Corps, Military Post 02149, Israel
H. Bercovier
Affiliation:
Israel Defense Force, Medical Corps, Military Post 02149, Israel Department of Clinical Microbiology, Hadassah Medical School, Hebrew University, Jerusalem
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The DNAs of Shigella sonnei or Shigella dysenteriae type 1 strains isolated in outbrealis of shigellosis or in sporadic cases were analysed by restriction fragment length polymorphism (RFLP). Southern blots of the DNAs of 36 S. sonnei isolates digested by 8 restriction enzymes were hybridized with an Escherichia coli rRNA probe. The S. sonnei strains were unexpectedly diverse in their RFLP. Antibiotypes of the same isolates showed clusters of strains corresponding to the various outbreaks. On the other hand, RFLP analysis suggested concomitant multiple sources of infection rather than a common source and thereby introduced a new insight in the epidemiology of shigellosis. RFLP was also used to trace S. dysenteriae type 1 transmission in a recent cluster of clinical cases. Although antibiotic resistance patterns indicated the presence of more than one strain, RFLP analysis showed that the six isolates were identical clones and suggested the loss of an R episome after one person-to-person passage.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 2 , October 1992 , pp. 273 - 282
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Keusch, GT, Bennish, ML. Shigellosis. In: Evans, AS, Brachman, PS, eds. Bacterial infections of humans. Epidemiology and control. 1991: 593–621.CrossRefGoogle Scholar
2.Green, MS, Block, C, Cohen, D, Slater, P. Four decades of shigellosis in Israel – The epidemiology of a growing public health problem. Rev Infect Dis 1991; 13: 248–53.CrossRefGoogle ScholarPubMed
3.Eisenstein, BI. New molecular techniques for microbial epidemiology and diagnosis of infectious diseases. J Infect Dis 1990; 161: 595–602.CrossRefGoogle ScholarPubMed
4.Aber, RC, Mackel, DC. Epidemiologic typing of nosocomial microorganisms. Am J Med 1981; 70: 161–7.CrossRefGoogle ScholarPubMed
5.Mendoza, MC, Gonzalez, AJ, Mendez, FJ, Hardisson, C. Plasmid typing of Shigella sonnei epidemic strains and molecular relationship of their R-plasmids. Eur J Epidemiol 1988; 4: 158–63.CrossRefGoogle ScholarPubMed
6.Litwin, CM, Ryan, KJ, Chipowsky, S, Storm, A, McCombie, S. Molecular epidemiology of Shigella sonnei in Pima County, Arizona: Evidence for a Mexico-related plasmid. J Infect Dis 1990; 161: 797–800.CrossRefGoogle ScholarPubMed
7.Tacket, CO, Shahid, N, Huk, MI, Alim, A, Cohen, MI. Usefulness of plasmid profiles for differentiation of Shigella isolates in Bangladesh. J Clin Microbiol 1984; 20: 300–1.CrossRefGoogle ScholarPubMed
8.Shlaes, DM, Currie-McCumber, CA. Plasmid analysis in molecular epidemiology: a summary and future directions. Rev Infect Dis 1986; 8: 738–46.CrossRefGoogle ScholarPubMed
9.Shaberg, DR, Rubens, CE, Alford, RH, Ferrar, WE, Schaffner, W, McGee, ZA. Evolution of antimicrobial resistance and nosocomial infections. Lessons from the Vanderbilt experience. Am J Med 1981; 70: 445–8.CrossRefGoogle Scholar
10.Stull, TL, Lipuma, JJ, Edlind, TD. A broad spectrum-probe for molecular epidemiology of bacteria; Ribosomal RNA. J Infect Dis 1988; 157: 280–5.CrossRefGoogle ScholarPubMed
11.Lipuma, JJ, Stull, TL, Dasen, SE, Pidcock, KA, Kaye, D, Korzeniowski, OM. DNA polymorphism among Escherichia coli isolated from bacteriuric women. J Infect Dis 1989; 159: 526–32.CrossRefGoogle ScholarPubMed
12.Kelly, MT, Brenner, DJ, Farmer, JJ III. Enterobacteriaceae. In: Lennette, EH, Balows, A, Hausler, WJ Jr, Shadomy, HJ, eds. Manual of clinical microbiology, 4th edn.Washington, D.C.: American Society for Microbiology, 1986: 263–77.Google Scholar
13.Brenner, DJ, McWhorther, AC, Leete-Knutson, JK, Steigerwalt, AG. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 1982; 15: 1133–40.CrossRefGoogle ScholarPubMed
14.Brosius, I, Ullrich, A, Raker, MA, Gray, A, Dull, TJ, Gutell, RR, Noller, HF. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid 1981; 6: 112–8.CrossRefGoogle ScholarPubMed
15.Sambrook, J, Fritsch, EF, Maniatis, T. Plasmid vectors. In: Sambrook, J, Fritsch, EF, Maniatis, T, eds. Molecular cloning. A laboratory manual, 2nd edn.Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989: 1.1–1.110.Google Scholar
16.Karpel, R, Olami, Y, Taglicht, D, Schuldiner, S, Padan, E. Sequencing of the gene ant which affects the Na/H antiporter activing in Escherichia coli. J Biol Chem 1988; 263: 10408–14.CrossRefGoogle Scholar
17.Sambrook, J, Fritsch, EF, Maniatis, T. Analysis and cloning of eukaryotic genomic DNA. In: Sambrook, J, Fritsch, EF, Maniatis, T, eds. Molecular cloning. A laboratory manual, 2nd edn.Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 1989: 9.1–9.Google Scholar
18.Bauer, AW, Kirby, WWM, Sherris, JC, Turck, M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 1966; 45: 493–6.CrossRefGoogle Scholar
19.Frost, JA, Vandepite, J, Rowe, B, Threlfall, EJ. Plasmid characterization in the investigation of an epidemic caused by multiple resistant Shigella dysenteriae type 1 in Central Africa. Lancet 1981; 2: 1074–6.CrossRefGoogle Scholar
20.Tompkins, LS. DNA methods in clinical microbiology. In: Lennett, EH, Balows, A, Hausler, WJ Jr, Shadomy, HJ, eds. Manual of clinical microbiology, 4th edn.Washington, D.C.: American Society for Microbiology, 1985: 1023–8.Google Scholar
21.Hinojosa-Ahumada, M, Swaminathan, B, Hunter, SB, Cameron, DN, Kiehlbauch, A, Wachsmuth, IK, Strockbine, NA. Restriction fragment length polymorphisms in rRNA operons for subtyping Shigella sonnei. J Clin Microbiol 1991; 29: 2380–4.CrossRefGoogle ScholarPubMed
22.Strockbine, NA, Parsonet, J, Greene, K, Kiehlbauch, JA, Wachsmuth, IK. Molecular epidemiologic techniques in analysis of epidemic and endemic Shigella dysenteriae type I strains. J Infect Dis 1991; 163: 406–9.CrossRefGoogle Scholar