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Preconditioning, but not postconditioning, with Sevoflurane reduces pulmonary neutrophil accumulation after lower body ischaemia/reperfusion injury in rats

Published online by Cambridge University Press:  01 June 2008

R. Kalb ,
P. Schober ,
L. A. Schwarte ,
J. Weimann  and
S. A. Loer
R. Kalb
Affiliation:
Heinrich-Heine-University Düsseldorf, Department of Anaesthesiology, Düsseldorf, Germany
P. Schober*
Affiliation:
VU Medisch Centrum, Department of Anaesthesiology, Amsterdam, The Netherlands
L. A. Schwarte
Affiliation:
VU Medisch Centrum, Department of Anaesthesiology, Amsterdam, The Netherlands
J. Weimann
Affiliation:
VU Medisch Centrum, Department of Anaesthesiology, Amsterdam, The Netherlands
S. A. Loer
Affiliation:
VU Medisch Centrum, Department of Anaesthesiology, Amsterdam, The Netherlands
*
Correspondence to: Patrick Schober, Department of Anaesthesiology, VU Medisch Centrum, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. E-mail: P.Schober@vumc.nl; Tel: +31 (0) 20 444 3138; Fax: +31 (0) 20 444 4385
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Summary

Background and objectives

Aortic ischaemia and reperfusion may induce pulmonary sequestration of neutrophil granulocytes. Preconditioning and postconditioning with volatile anaesthetics confer protection against reperfusion injury in various organs, such as heart, kidneys or brain. We tested the hypothesis that pre- or postconditioning with Sevoflurane attenuates pulmonary neutrophil accumulation after ischaemia/reperfusion injury of the aorta.

Methods

Anaesthetized and mechanically ventilated Wistar rats underwent laparotomy and were randomly assigned to one of the following groups: Sham (n = 10), ischaemia/reperfusion (n = 8, lower body ischaemia by clamping of the infrarenal aorta for 2 h followed by 3 h of reperfusion), preconditioning (n = 10, 2.0% Sevoflurane administered over 30 min prior to ischaemia) and postconditioning (n = 9, 2.0% Sevoflurane during reperfusion). Following reperfusion, the lungs were removed for microscopic determination of neutrophil accumulation.

Results

Ischaemia/reperfusion induced a significant increase in pulmonary neutrophil accumulation (mean ± SD, 29.9 ± 7.4 vs. 15.8 ± 6.6 neutrophils per microscopic field in ischaemia/reperfusion vs. Sham, respectively, P < 0.001). Sevoflurane preconditioning resulted in a lower neutrophil count (20.3 ± 7.1 neutrophils, P < 0.001 vs. ischaemia/reperfusion), while postconditioning showed no effects (25.8 ± 9.8 neutrophils vs. ischaemia/reperfusion, not significant).

Conclusions

Preconditioning, but not postconditioning, with Sevoflurane reduces pulmonary neutrophil accumulation after ischaemia/reperfusion injury of the lower body. Since neutrophil accumulation plays a major role in the pathophysiology of acute lung injury, our data suggest a protective effect of Sevoflurane preconditioning on remote pulmonary ischaemia/reperfusion injury.

Keywords

ANAESTHESIA INHALATIONSEVOFLURANELEUCOCYTESNEUTROPHILSLUNG FUNCTIONISCHAEMIA REPERFUSION
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 25 , Issue 6 , June 2008 , pp. 454 - 459
Copyright
Copyright © European Society of Anaesthesiology 2008

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