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Alfentanil attenuates phenylephrine-induced contraction in rat aorta

Published online by Cambridge University Press:  01 March 2007

J.-T. Sohn ,
K.-E. Park ,
C. Kim ,
Y.-S. Jeong ,
I.-W. Shin ,
H.-K. Lee  and
Y.-K. Chung
J.-T. Sohn
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea Institute of Health Sciences, Chilam-dong, Jinju, Gyeongnam, South Korea
K.-E. Park
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
C. Kim
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
Y.-S. Jeong
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
I.-W. Shin
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
H.-K. Lee
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
Y.-K. Chung
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
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Summary

Background and objectives

Alfentanil was reported to relax the rat aorta by direct action on the vascular smooth muscle. The aims of this in vitro study were to examine the effect of alfentanil on phenylephrine-induced contractions in the rat aorta and to determine the cellular mechanism associated with this process.

Methods

Endothelium-denuded aortic rings were suspended in order to record isometric tension. In the rings with or without 10−6 mol naloxone or 10−5 mol verapamil, the concentration–response curves for phenylephrine and potassium chloride were generated in the presence or absence of alfentanil (10−6, 5 × 10−5, 10−4 mol). In the rings exposed to a calcium-free isotonic depolarizing solution, the contractile response induced by the addition of calcium was assessed in the presence or absence of alfentanil (5 × 10−5, 10−4 mol).

Results

Alfentanil (5 × 10−5, 10−4 mol) attenuated (P < 0.05) the phenylephrine-induced contraction in the ring with or without 10−6 mol naloxone but had no effect on the phenylephrine-induced contraction in the rings pretreated with verapamil. Alfentanil (5 × 10−5, 10−4 mol) produced a significant rightward shift (P < 0.01) in the potassium chloride dose–response curve, and attenuated the contractile response (P < 0.001) induced by calcium in the calcium-free isotonic depolarizing solution in a dose-dependent manner.

Conclusions

A supraclinical dose of alfentanil attenuates the phenylephrine-induced contraction via an inhibitory effect on calcium influx by blocking the l-type calcium channels in the rat aortic vascular smooth muscle.

Keywords

ANAESTHETICS INTRAVENOUS, alfentanilAORTA, contraction, smooth muscleADRENERGIC ALPHA-AGONISTS, phenylephrinePOTASSIUM CHLORIDEVERAPAMILRAT
Type
Research Article
Information
European Journal of Anaesthesiology , Volume 24 , Issue 3 , March 2007 , pp. 276 - 282
Copyright
Copyright © European Society of Anaesthesiology 2006

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