Adenylate-cyclase Activity in Obsessive-compulsive Patients

Abstract

Introduction

A possible role of second messengers, such as cyclic adenosine monophosphate (cAMP) signalling, in the development of obsessive-compulsive disorder (OCD) has been recently postulated.

Aims

The aim of the present study was to explore and to compare the adenylate cyclase (AC) activity in both basal conditions and after the stimulation by isoprenaline (ISO) in platelets of OCD patients and healthy control subjects. The AC activity was measured both in the absence and in the presence of α- and β- adrenoreceptor antagonists.

Materials and methods

Forty patients were included in the study and compared with healthy volunteers. Biological assays were carried out with a method developed by us.

Results

The basal AC activity was similar in both groups. The addition of 10 μM ISO enhanced significantly (P < .05) platelet basal AC in both groups. A stimulatory response following ISO in all subjects even without α-antagonists was also observed.

Discussion

No difference in the basal AC activity in platelet membranes of healthy subjects and OCD patients was found. Our findings showed that there is an inhibitory component of ISO effect on platelet AC, due to the agonist interaction with α2 receptors, at its higher concentrations (>1 μM), as well as a condition of supersensitive β-receptors. Our study suggests the presence of cathecolamine system disturbances in OCD.

Disclosure of interest

The authors have not supplied their declaration of competing interest.