P01-379 - Study on the Intestinal Endotoxemia in the Cell Apoptosis of Alzheimer Disease Rats

Abstract

Objective

The objective of the present study was to explore the intestinal endotoxemia (IETM) in the cell apoptosis of Alzheimer disease's rats which were established by D-galactose and aluminum trichloride (AlCl₃).

Methods

Adult wistar rats were subjected to 90 days of intraperitoneal injection with D-galactose and AlCl₃ to establish the Alzheimer disease's model. After the administration, the study and memory ability of the Alzheimer disease's rats were observed by Morris water maze; The level of Lipopolysaccharide (LPS) in the sera of Alzheimer disease's rats was determined by tachypleus amebocyte lysate method; The level of tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) in the sera of Alzheimer disease's rats were determined by radioimmunity method; The apoptotic neuron was detected by TdT-mediated dUTP Nick End Labeling (TUNEL).

Results

Compared with the normal control, the level of LPS, TNF-αin the sera and PD in the brine of Alzheimer disease's rats were markedly increased (P< 0.01).

Conclusions

The level of LPS, TNF-αin the sera and PD in the brine of Alzheimer disease's rats were markedly increased, IETM maybe an important reason of the apoptotic in Alzheimer disease.