PW01-80 - The Effects On The Level Of Intestinal Endotoxemia In Alzheimer Disease Rats

Abstract

Aim

The objective of the study was to explore whether IETM participate in the development of Alzheimer disease's rats which were established by D-galactose and aluminum trichloride (AlCl₃).

Methods

Adult Wistar rats were subjected to 90 days of intraperitoneal injection with D-galactose and AlCl₃ to establish the Alzheimer disease's model. After the administration, the study and memory ability of the Alzheimer disease's rats were observed by Morris water maze; The level of Lipopolysaccharide (LPS) in the sera of Alzheimer disease's rats was determined by tachypleus amebocyte lysate method; The level of tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) in the sera of Alzheimer disease's rats were determined by radioimmunity method; The express of amyloid β-protein precursor (APP), presenilin 1 (PS1) and β-site APP-cleaving enzyme (BACE) in hippocampus of Alzheimer disease's rats were detected by RT-PCR.

Results

Compared with the normal control, the level of LPS in the sera and the express of APP, PSI, BACE mRNA in the hippocampus of Alzheimer disease's rats were markedly increased (P< 0.01).

Conclusions

The model of Alzheimer disease's rats which were established by D-galactose and AlCl₃ is accompanied IETM. This result suggests that IETM play an important role in the development of Alzheimer disease.