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LIQUID PRESERVATION OF CUCURBIT SEEDS AT AMBIENT TEMPERATURE

Published online by Cambridge University Press:  31 August 2016

DAYA K. PANDEY
DAYA K. PANDEY*
Affiliation:
Indian Council of Agricultural Research - Directorate of Weed Research, Maharajpur, Jabalpur 482 004, Madhya Pradesh, India
*
‡Corresponding author. Email: dayapandey@hotmail.com
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Summary

Low-cost methods for enhancement of seed longevity may facilitate biodiversity conservation especially in resource constrained situations. Longevity of 13 cucurbitaceous seed lots belonging to 12 species with mean moisture contents 9.3±0.7% was studied in hermetic storage (HS) and in a liquid consisting of 20% (w/w) CaCl2 in glycerol, following seed to preservative ratio of 1:1 (v/v), at ambient temperature for 24 years. The liquid preservative (LP) was not absorbed by the seeds over the storage period and was harmless to them, drying seeds to about 5% of moisture and maintaining them at this state throughout the storage period. The seeds lost viability in HS in 3.8±0.4 years, while in LP viability was lost in 14–16 years in eight species and the seeds were still viable in five species after 24 years. LP-preserved seeds and also fresh seeds of three species were planted under field conditions. The results validated the use of LP in seed storage for 24 years. The liquid preservation extended longevity of the cucurbit seeds at ambient temperature by drying them and probably maintaining them in oxygen-free environment.

Type
Research Article
Information
Experimental Agriculture , Volume 53 , Issue 4 , October 2017 , pp. 553 - 565
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Copyright
Copyright © Cambridge University Press 2016 

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References

REFERENCES

Anonymous (1997). Dust control on unpaved roads. Wisconsin Transportation Bulletin 13:1–6. Available at: http://epdfiles.engr.wisc.edu/pdf_web_files/tic/bulletins/Bltn_013_DustControl.pdf. Accessed on 11 January 2016.Google Scholar
Anonymous (2002a). Calcium chloride. OECD SIDS. UNEP Publication, pp. 1–154. Available at: http://www.chem.unep.ch/irptc/sids/OECDSIDS/10043524.pdf. Accessed on 11 January 2016.Google Scholar
Anonymous (2002b). Glycerol. OECD SIDS, 1–178. Available at: http://www.chem.unep.ch/irptc/sids/OECDSIDS/56815.pdf. Accessed on 11 January 2016.Google Scholar
Bewley, J. D. and Black, M. (Eds). (1982). Viability and longevity. In Physiology and Biochemistry of Seed in Relation to Germination. II. Viability, Dormancy and Environmental Control, 1–59. Berlin: Springer-Verlag.Google Scholar
Buitink, J. and Leprince, O. (2008). Intracellular glasses and seed survival in the dry state. Comptes Rendus Biologies 331:788–795.Google Scholar
Corvalan, C., Hales, S. and McMichael, A. (2005). Millennium ecosystem assessment. Ecosystems and human well-being: Biodiversity Synthesis, Washington, DC, USA: World Resources Institute.Google Scholar
Ellis, R. H. and Hong, T. D. (2007). Seed longevity- moisture content relationships in hermetic and open storage. Seed Science and Technology 35:423–431.Google Scholar
EPA (United States Environmental Protection Agency) (2000). N, N-Dimethylaniline. Technology Transfer Network – Air Toxics Web Site. U.S. Environmental Protection Agency. Available at: http://www.epa.gov/ttnatw01/hlthef/dime-lin.html. Accessed on 11 January 2016.Google Scholar
Esquinas-Alcazar, J. (2005). Protecting crop genetic diversity for food security: Political, ethical and technical challenges. Nature Reviews – Genetics 6:946–953.Google Scholar
Fassil, H. and Engels, J. (1997). Seed Conservation Research: IPGRI's Strategies and Activities. Available at: http://www.bgci.org/worldwide/article/349/. Accessed on 11 January 2016.Google Scholar
Fisher Scientific – Silica Gel Desiccant (Sic) (1997). Fisher Scientific – MSDS. Available at: http://www.atmos.umd.edu/~russ/MSDS/silicagel28200.html. Accessed on 11 January 2016.Google Scholar
Gomez-Campo, C. (2007). A Guide to Efficient Long Term Seed Preservation. Monographs ETSIA, University Politecnia de Madrid 170, 1–17.Google Scholar
Gomez-Campo, C. (2009). Efficient Long Term Seed Preservation. Monographs ETSIA, Universidad Politecnica de Madrid 171, 1–3.Google Scholar
Groot, S. P. C., De Groot, L., Kodde, J. and van Treuren, R. (2014). Prolonging the longevity of ex situ conserved seeds by storage under anoxia. Plant Genetic Resources, 13:18–26.CrossRefGoogle Scholar
Groot, S. P. C., Surki, A. A., de Vos, R. C. H. and Kodde, J. (2012). Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions. Annals of Botany, 110:1149–1159.Google Scholar
IBPGR (1985). International Board of Plant Genetic Resources. Cost-effective Long-term Seed Stores, Rome: IBPGR.Google Scholar
ISTA (International Seed Testing Association) (1985). International rules for seed testing. Seed Science and Technology 13:356–513.Google Scholar
Kibinza, S., Vinel, D., Côme, D., Bailly, C. and Corbineau, F. (2006). Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum 128:496–506.Google Scholar
Koostra, P. T. and Harrington, J. F. (1969). Biochemical effect of age on membranal lipids of Cucumis sativus L. seed. Proceedings of the International Seed Testing Association 34:329–340.Google Scholar
Li, D. Z. and Pritchard, H. W. (2009). The science and economics of ex situ plant conservation. Trends in Plant Science 14:614–621.Google Scholar
Lison, D., Boeck, M. D., Verougstrate, V. and Kirsch-Volders, M. (2001). Update on the genotoxicity and carcinogenicity of cobalt compounds. Occupational and Environmental Medicine 58:619–625.Google Scholar
Matsuda, H. and Hirayama, O. (1973). Changes of lipid components and lipolytic acylhydrolase activities in rice grains during their storage. Journal of Agricultural Chemistry Society of Japan 47:279–384.Google Scholar
Mcdonald, M. B. (1999). Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27:177–237.Google Scholar
Murthy, U. M. N., Kumar, P. P. and Sun, W. Q. (2003). Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. Journal of Experimental Botany 54, 1057–1067.Google Scholar
NCBI (National Centre for Biotechnology Information) (2016). PubChem Compound Database; CID=24261. Available at: https://pubchem.ncbi.nlm.nih.gov/compound/24261. Accessed on 11 January 2016.Google Scholar
OSHA (Occupational Safety and Health Administration) (2006). United States Department of Labour. Available at: https://www.osha.gov/. Accessed on 11 January 2016.Google Scholar
Pandey, D. K. (1989). Ageing of French bean seeds at ambient temperature in relation to vigour and viability. Seed Science and Technology 17, 41–47.Google Scholar
Pandey, D. K. (1994). Improved longevity of onion (Allium cepa L.) seeds by immersing in hexylene glycol. Scientia Horticulturae - Amsterdam 59:297–302.CrossRefGoogle Scholar
Pandey, D. K. (1995). Liquid preservative to improve longevity of tomato (Lycopersicum esculentum L.) seeds. Scientia Horticulturae - Amsterdam 62:57–62.Google Scholar
Pandey, D. K. (1996). A suitable liquid preservative for enhancing longevity of orthodox seeds. Scientia Horticulturae - Amsterdam 66:1–8.Google Scholar
Pearce, R. S. and Abdel-Samad, I. M. (1980). Changes in fatty acid content of polar lipids during ageing of seeds of peanut (Arachis hypogeal L.). Journal of Experimental Botany 31:1283–1290.Google Scholar
Perez-Garcia, F., Gonzalez-Benito, M. E. and Gomez-Campo, C. (2007). High viability records in ultra-dry seeds of 37 species of Brassicaceae after almost 40 years of storage. Seed Science and Technology 35:143–153.Google Scholar
Perez-Garcia, F., Gonzalez-Benito, M. E. and Gomez-Campo, C. (2008). Germination of fourteen endemic species of Iberian Peninsula, Canary and Balearic Islands after 32-34 years of storage at low temperature and very low moisture content. Seed Science and Technology 36:407–422.Google Scholar
Petruzzelli, L. and Taranto, G. (1984). Phospholipid changes in wheat embryos aged under different storage conditions. Journal of Experimental Botany 35:517–520.Google Scholar
Priestley, D. A. (1986). Seed Ageing, Ithaca, New York: Cornell University Press.Google Scholar
Priestley, D. A. and Leopold, A. C. (1983). Lipid changes during natural ageing of soybean seeds. Physiologia Plantarum 59:467–470.Google Scholar
Rao, N. K., Hanson, J., Dulloo, M. E., Ghosh, K., Nowell, D. and Larinde, M. (2006). Manual of seed handling in genebanks. Handbooks for Genebanks No. 8. Rome, Italy: Diversity International.Google Scholar
Roos, Y. H. (1998). Phase transitions and structure of solid food matrices. Current Opinion in Colloid & and Interface Science 3:651–656.Google Scholar
Schoen, D. J. and Brown, A. H. D. (2001). The conservation of wild plant species in seed banks – attention to both taxonomic coverage and population biology will improve the role of seed banks as conservation tools. Bioscience 51:960–966.Google Scholar
Schwember, A. R. and Bradford, K. J. (2011). Oxygen interacts with priming, moisture content and temperature to affect the longevity of lettuce and onion seeds. Seed Science Research, 21:175–185.Google Scholar
Smith, M. T. and Berjak, P. (1995). Deteriorative changes associated with the loss of viablityviability of stored desiccation-tolerant and desiccation-sensitive seeds. In: Seed Development and Germination, 701–746 (Eds Kigel, J. and Galili, G.). New York: Marcel Dekker., Inc.Google Scholar
Stanwood, P. C. (1985). Cryopreservation of seed germplasm for genetic conservation. In Cryopreservation of Plant Cells and Organs, 199–226 (Ed Kartha, K. K.). Boca Raton: CRC Press.Google Scholar
Sukhdev, P. (2008). The economics of ecosystems and biodiversity. An Interim Report, European Communities. Cambridge, UK: A Banson Production. ISBN-13 978-92-79-08960-2.Google Scholar
Sun, W. Q. (1997). Glassy state and seed storage stability: the WLF kinetics of seed viability loss at T>Tg and the plasticization effect of water on storage stability. Annals of Botany 79:291–297.Google Scholar
Walters, C. (1998). Understanding the mechanisms and kinetics of seed ageing. Seed Science Research 8:223–244.Google Scholar
Walters, C. and Engels, J. (1998). The effects of storing seeds under extremely dry conditions. Seed Science Research 8 (supplement. 1):3–8.Google Scholar
Wilson, J. R. D. O. and McDonald, J. R. M. B. (1986). The liquid peroxidation model of seed ageing. Seed Science and Technology 14:269–300.Google Scholar
Zheng, G. H., Jing, X. M. and Tao, K. L. (1998). Ultra dry seed storage cuts cost of gene banks. Nature 393:223–224.Google Scholar