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SEMI-SELECTIVE MEDIUM FOR ISOLATION OF COLLETOTRICHUM GLOEOSPORIOIDES FROM SOIL

Published online by Cambridge University Press:  01 July 2000

E. J. EKEFAN ,
S. A. SIMONS ,
A. O. NWANKITI  and
J. C. PETERS
E. J. EKEFAN
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT, United Kingdom
S. A. SIMONS
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT, United Kingdom
A. O. NWANKITI
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT, United Kingdom
J. C. PETERS
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT, United Kingdom
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Abstract

Isolation of Colletotrichum gloeosporioides (causal agent of yam anthracnose) from soil is difficult because of the presence of fast growing micro-organisms like Rhizoctonia spp., Rhizopus spp., Penicillium spp. and bacteria. Therefore, a semi-selective medium consisting of antimicrobial agents such as pencycuron 50 mg L−1, tolclofos-methyl 10 mg L−1, streptomycin sulphate 100 mg L−1, chloramphenicol 100 mg L−1 and chlortetracycline 100 mg L−1 was developed using potato dextrose agar as the basal medium. The procedure involved screening of candidate fungicides, developing a suitable combination of antimicrobial agents, assessing the proposed semi-selective medium and adjustment of pH of the medium to 5. The effectiveness of the medium is due to selective inhibition of micro-organisms by the antimicrobial agents and the development of distinct salmon-pink colonies of C. gloeosporioides.

Type
Research Article
Information
Experimental Agriculture , Volume 36 , Issue 3 , July 2000 , pp. 313 - 321
Copyright
© 2000 Cambridge University Press

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