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Diagnosis of Mycosphaerella spp., responsible for Mycosphaerella leaf spot diseases of bananas and plantains, through morphotaxonomic observations

Published online by Cambridge University Press:  29 November 2008

Marie-Françoise Zapater ,
Catherine Abadie ,
Luc Pignolet ,
Jean Carlier  and
Xavier Mourichon
Marie-Françoise Zapater
Affiliation:
CIRAD-Bios, UMR BGPI, TA A 54 / K, 34398, Montpellier Cedex 5, France
Catherine Abadie
Affiliation:
CIRAD-Bios, UPR Mult. Vég., Stn. Neufchâteau, 97130, Capesterre Belle-Eau, Guadeloupe, France
Luc Pignolet
Affiliation:
CIRAD-Bios, UMR BGPI, TA A 54 / K, 34398, Montpellier Cedex 5, France
Jean Carlier
Affiliation:
CIRAD-Bios, UMR BGPI, TA A 54 / K, 34398, Montpellier Cedex 5, France
Xavier Mourichon
Affiliation:
CIRAD-Bios, UMR BGPI, TA A 54 / K, 34398, Montpellier Cedex 5, France
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Abstract

Introduction. This protocol aims to diagnose under laboratory conditions the main Mycosphaerella spp. pathogens of bananas and plantains. The three pathogens Mycosphaerella fijiensis (anamorph Paracercospora fijiensis), M. musicola (anamorph Pseudocercospora musae) and M. eumusae (anamorph Pseudocercospora eumusae) are, respectively, the causal agents of Black Leaf Streak disease, Sigatoka disease and Eumusae Leaf Spot disease. The principle, key advantages, starting plant material and time required for the method are presented. Materials and methods. The laboratory materials required and details of the thirteen steps of the protocols (tissue clearing and in situ microscopic observations, isolation on artificial medium and cloning of single-spore isolate, in vitro sporulation and microscopic observations of conidia, and long-term storage of isolates) are described. Results. Diagnosis is based on the observations of anamorphs (conidiophores and conidia) which can be observed directly from banana leaves or after sporulation of cultivated isolates if sporulating lesions are not present on banana samples.

Keywords

FranceMusa sp.Mycosphaerella fijiensisMycosphaerella musicolaMycosphaerella eumusaefoliar diagnosismicroscopyFranceMusa sp.Mycosphaerella fijiensisMycosphaerella musicolaMycosphaerella eumusaediagnostic foliairemicroscopie
Type
Research Article
Information
Fruits , Volume 63 , Issue 6 , November 2008 , pp. 389 - 393
Copyright
© CIRAD, EDP Sciences, 2008

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References

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