Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-27T15:10:00.223Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunochemical ablation of accessory reproductive glands of the male desert locust

Published online by Cambridge University Press:  19 September 2011

T. S. Dhadialla ,
Thomas R. Odhiambo  and
G. G. Wagner
T. S. Dhadialla
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Thomas R. Odhiambo
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
G. G. Wagner
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Get access

Abstract

Injection of antiserum prepared against accessory reproductive gland (ARG) secretions of the male desert locust, Schistocerca gregaria, disrupted the normal growth and function of the ARG complex in sexually maturing adults. Male locusts were injected with either antiserum or normal guinea-pig serum on days 3, 7 and 10 after adult ecdysis. When the injection of ARG antiserum began as early as day 3 of adult development a marked inhibition of ARG growth was accompanied by the suppression of other characteristics of sexual maturation (yellow body coloration and mating behaviour). Inhibition of ARG growth, while significant, was less pronounced when antiserum injections began on day 7 of adult development, and the injected males did not exhibit normal mating behaviour when placed with virgin females. When single antiserum injections were administered on day 10 of adult development, the males still attained their yellow body coloration by day 19 and copulated normally with virgin females, although their ARGs were only equivalent in size to those 13-day-old males.

Résumé

L'injection de l'antiserum préparé contre les sécretions de la glande reproductrice auxilliair (ARG) des locustes maies du desert, Schistocerca gregaria, interompt la eroissance normale et le fouctionnement du complexe ARG chezles adultes sexuellement matures. Les locustes mâles sont injectes auee aussi bien l'antiserum qu'avec le serum normal de cobaye aux jours 3, 7 et 10 aprés la transformation adulte. Quand l'injection de l'antiserum ARG commence tres tôt au jour 3 du developpment adulte, une inhibition, marquée de la croissance de l'ARG est accompagnée par la suppression des autres caracteristiques de la maturation sexuelle (une coloration jamme du corps, et un comportement sexuel). L'inhibition de la croissance de l'ARG quoique significative, est moius pronnoucée quand l'injection de l'antiserum de'bute au jour 7 du du developpment adulte et les maies injecte's néxhibeut pas de compartement sexuel normal quand ils sout placés en presence de females. Quand des injections uniques de l'antiserum sout adminstrées au jour 10 du developpment adulte, les maies gardent eucore leur coloration corporelle jaune jusqu'au jour 19. Et s'accouplent normalement avec les females vierges bien que leurs ARGs sont seulement equivalents en grandeur a' ceux des males ages de 13 jours.

Keywords

Immunochemical ablationaccessory reproductive glandsdesert locustAblation immunochimiqueglandes reproductriees auxilliariesdes locustes du desert
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 7 , Issue 4 , August 1986 , pp. 465 - 470
Copyright
Copyright © ICIPE 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alger, E. N. and Cabrera, E. J. (1972) An increase in death rate of Anopheles stephensi fed on rabbits immunized with mosquito antigen. J. econ. Ent. 65, 165–168.CrossRefGoogle ScholarPubMed
Allen, J. R. and Humphreys, S. J. (1979) (Immunization of guinea pigs, and cattle against ticks. Nature 280, 491–493.CrossRefGoogle ScholarPubMed
Bunng, T., Campbell, C. M. and Idler, D. R. (1980) Antibody inhibition of vittellogenesis and oocyte maturation in salmon and flounder. Gen. comp. Endocr. 41, 233–239.CrossRefGoogle Scholar
Gregory, G. E. (1965) The formation and fate of the spermatophore in the African migratory locust, Locusta migratoria migratorioides Reiche and Fairmaire. Trans. R. ent. Soc. Lond. 117, 33–66.CrossRefGoogle Scholar
Hagedorn, H. H. and Kunkel, J. G. (1979) Vitellogenin and vitellin in insects. A. Rev. Ent. 24, 475–505.CrossRefGoogle Scholar
Hartmann, R. (1970) Experimentelle und histologische Untersuchungen der spermatophorenbildung beki der Feldheuschrecke Gomphocerus rufus L. (Ortheptera;Acrididae). Z. Morph. Tiere. 68, 140–176.CrossRefGoogle Scholar
Hunter-Jones, P. (1966) Rearing and Breeding Locusts in the Laboratory. Anti-Locust Research Centre, London.Google Scholar
Kaaya, G. P. and Alemu, P. (1982) Fecundity and survival of tsetse maintained on immunized rabbits. Insect Sci. Applic. 3, 237–241.Google Scholar
Loher, W. (1961) The chemical acceleration of the maturation process and its hormonal control in the male of the desert locust. Proc. R. Soc. B 153, 380–397.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275.CrossRefGoogle ScholarPubMed
Norris, M. J. (1954) Sexual maturation in the desert locust (Schistocerca gregaria Forskal) with special reference to the effects of grouping. Anti-Locusts Bull. 18, 1–44.Google Scholar
Odhiambo, T. R. (1966) Growth and hormonal control of sexual maturation in the male desert locust, Schistocerca gregaria Forskal. Trans. R. ent. Soc. Lond. 118, 393–412.CrossRefGoogle Scholar
Odhiambo, T. R. (1969) The architecture of the accessory reproductive glands of the male desert locust. I. Types of glands and their secretions. Tissue Cell 1, 155–182.CrossRefGoogle ScholarPubMed
Pener, M. P. (1965) On the influence of corpora allata on maturation and sexual behaviour of Schistocerca gragaria. J. Zool. 147, 119–136.Google Scholar
Pener, M. P. (1967) Effects of allatectomy and sectioning of the nerves of the corpora allata on oocyte growth, male sexual behaviour and colour change in adults of Schistocerca gregaria. J. Insect Physiol. 13, 665–684.CrossRefGoogle Scholar
Pickford, R. and Padgham, D. (1973) Spermatophore formation and spermtransfer in the desert locust, Schistocerca gregaria (Orthoptera; Acrididae). Can. Ent. 105, 613–719.CrossRefGoogle Scholar
Rembold, H., Eder, J. and Ulrich, G. M. (1980) Inhibition of allatotropic activity and ovary development in Locusta migratoria by anti-brain-antibodies. Z. Naturforsch. 35c, 1117–1119.CrossRefGoogle Scholar
Scheidegger, J. J. (1955) Une micro-methode de limmuno-electrophrese. Inter. Arch. Allergy Appl. Immun. 7, 103–110.CrossRefGoogle Scholar
Schiein, Y. and Lewis, C. T. (1976) Lesions in hematophagus flies after feeding on rabbits immunized with fly tissues. Physiol. Ent. 1, 55–59.CrossRefGoogle Scholar
Shirk, P. D., Dahm, K. H. and Roller, H. (1976) The accessory sex glands as repository for juvenile hormone in male cecropia moths. Z. Naturforsch. 31c, 199–200.CrossRefGoogle Scholar
Shivers, C. A., Dudkiewicz, A. B., Franklin, L. E. and Fussell, E. N. (1972) Inhibition of sperm-egg interaction by specific antibody. Science 178, 1211–1213.CrossRefGoogle ScholarPubMed
Sutherland, G. B. and Ewen, A. B. (1973) Fecundity decrease in mosquitoes ingesting blood from specifically sensitized mammals. J. Insect Physiol. 20, 655–660.CrossRefGoogle Scholar
Wikel, S. K. (1982) Immune responses to arthropods and their products. A. Rev. Ent. 27, 21–48.CrossRefGoogle ScholarPubMed
Wikel, S. K. (1984) Immunomodulation of host responses to ectoparasite infestation-an overview. Vet. Parasit. 14, 321–339.CrossRefGoogle ScholarPubMed
Willadsen, P. (1980) Immunity to ticks. Adv. Parasit. 18, 293–313.CrossRefGoogle ScholarPubMed
Wyatt, G. R. and Pan, M.-L. (1978) Insect plasma proteins. A. Rev. Biochem. 47, 779–817.CrossRefGoogle ScholarPubMed