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Biochemical evidence for the feeding sites of the leafhopper Nephotettix virescens Within susceptible and resistant rice plants

Published online by Cambridge University Press:  19 September 2011

J. L. Auclair ,
E. Baldos  and
E. A. Heinrichs
J. L. Auclair*
Affiliation:
International Rice Research Institute, P.O. Box 933, Manila, the Philippines
E. Baldos
Affiliation:
International Rice Research Institute, P.O. Box 933, Manila, the Philippines
E. A. Heinrichs
Affiliation:
International Rice Research Institute, P.O. Box 933, Manila, the Philippines
*
*To whom correspondence should be addressed at: Département de Sciences Biologiques, Université de Montréal, Suce. A. C.P. 6128. Montréal, P.Q. Canada H3C 3J7.
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Abstract

Semi-quantitative biochemical analyses by paper chromatography–densitometry and pH determinations of honeydew excreted by Nephotettix virescens (Distant) feeding on rice plants revealed higher free amino compound and sugar concentrations in honeydew excreted on the susceptible variety TN1 than on resistant ASD7 or P203. Furthermore, sap exudates from these three varieties, presumed to be mainly from xylem, as well as honeydew excreted on the resistant varieties were acidic, whereas honeydew from TN1 was predominantly basic. Since phloem sap in herbaceous plants usually gives an alkaline reaction whereas xylem sap is acidic, and because the former contains much higher concentrations of sugars and free amino acids than the latter, the biochemical evidence strongly suggests that Nephotettix virescens is primarily a xylem feeder on the resistant plants tested, but feeds predominantly on phloem sap in susceptible TNI.

Résumé

Des analyses semi-quantitatives par chromatographie sur papier et densitométrie et des déterminations du pH ont été effectuées sur le miellat excrété par Nephotettix virescens (Distant) se nourrissant sur des plantes de riz. Les résultats ont indiqué une teneur beaucoup plus élevée en acides aminés libres et en sucre dans le miellat excrété sur la variété sensible de riz TN1 que sur les variétés résistantes ASD7 ou P203. De plus, des exsudats de sève brute provenant des vaisseaux du bois (xylème) de ces trois variétés, de même que le miellat excrété sur les variétés résistantes étaient de réaction acide, tandis que le miellat excrété sur la variété sensible était généralement alcalin. Puisque la sève élaborée du phloème chez les plantes herbacées est généralement de réaction alcaline, tandis que celle provenant des vaisseaux du bois est acide, et que la sève élaborée contient généralement une teneur beaucoup plus élevée en acides aminés et en sucres, les résultats d'analyse biochimique suggèrent fortement que Nephotettix virescens se nourrit principalement de la sève brute sur les deux variétés résistantes, mais surtout de sève élaborée chez la variété sensible TN1.

Keywords

Nephotettix virescensfeeding sitesrice plantsaphoneydewNephotettix virescenssites de prise de nourritureriz
Type
Articles
Information
International Journal of Tropical Insect Science , Volume 3 , Issue 1 , March 1982 , pp. 29 - 34
Copyright
Copyright © ICIPE 1982

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References

REFERENCES

Aimi, R. and Konno, S. (1955) Studies on pH of rice tissues. mimeo, Interim Research Report 1954, Department of Physiology and Genetics, National Institute of Agricultural Science. In Japanese.Google Scholar
Auclair, J. L. (1963) Aphid feeding and nutrition. A. Rev. Ent. 8, 439–490.CrossRefGoogle Scholar
Bollard, E. G. (1960) Transport in the xylem. A. Rev. Pl. Physiol. 11, 141–166.CrossRefGoogle Scholar
CaGampang, G. B., Cruz, L. J. and Juliano, B. O. (1971) Free amino acids in the bleeding sap and developing grain of the rice plant. Cereal Chem. 48, 533–539.Google Scholar
Cagampang, G. B., Pathak, M. D. and Juliano, B. O. (1974) Metabolic changes in the rice plant during infestation by the brown planthopper, Nilaparvata lugens Stål (Hemiptera:Delphacidae). Appl. Ent. Zool. 9, 174–184.Google Scholar
Carlé, P. and Moutous, G. (1965) Observations sur le mode de nutrition sur vigne de quatre espèces de Cicadelles. Annls. Épiphyt. 16, 333–354.Google Scholar
Cheng, C. H. and Pathak, M. D. (1972) Resistance to Nephotettix virescens in rice varieties. J. econ. Ent. 65, 1148–1153.CrossRefGoogle Scholar
Cheung, W. W. K. and Marshall, A. T. (1973) Water and ion regulation in Cicadas in relation to xylem feeding. J. Insect Physiol. 19, 1801–1816.CrossRefGoogle Scholar
Eschrich, W. (1975) 2. Sealing systems in phloem. In Transport in Plants I. Phloem Transport (Edited by Zim-Mermann, M. H. and Milburn, J. L.), Encycl. PI. Physiol., New Series, Vol. 1, pp. 39–56. Springer, New York.Google Scholar
Hagley, E. A. C. and Blackman, J. A. (1966) Site of feeding of the sugar froghopper, Aeneolamia varia saccharia (Homoptera:Cercopidae). Ann. ent. Soc. Am. 59, 1289–1291.CrossRefGoogle Scholar
Harris, G. and MacWilliam, I. C. (1954) A dipping technique for revealing sugars on paper chromatograms. Chemy Ind. 9, 249.Google Scholar
Horsfield, D. (1978) Evidence for xylem feeding by Philaenus spumarius (L.) (Homoptera:Cercopidae). Entomologia exp. appl. 24, 95–99.CrossRefGoogle Scholar
International Rice Research Institute (IRRI) (1979) Research Highlights for 1978, pp. 27–28. Los Banos, Laguna, the Philippines.Google Scholar
Karim, A. N. M. R. (1978) Varietal resistance of rice to green leafhopper, Nephotettix virescens (Distant): sources, mechanisms, and genetics of resistance. Ph.D. thesis, University of the Philippines at Los Banos, the Philippines.Google Scholar
Kawabe, S. and McLean, D. L. (1980) Electronic measurement of probing activities of the green leafhopper of rice. Entomologia exp. appl. 27, 77–82.CrossRefGoogle Scholar
Ling, K. C. (1968) Mechanism of tungro-resistance in rice variety Pankhari 203. Philipp. Phytopath. 4, 21–38.Google Scholar
Ling, K. C. (1981) Rice virus and virus-like diseases. International Rice Research Institute, Los Banos, the Philippines.Google Scholar
Naito, A. (1977) Feeding habits of leafhoppers. Jap. agric. Res. Q. 11, 116–119.Google Scholar
Noda, H., Sogawa, K. and Saito, T. (1973) Amino acids in honeydews of the rice planthoppers and leafhoppers (Homoptera:Delphacidae, Deltocephalidae). Appl. Ent. Zool. 8, 191–197.CrossRefGoogle Scholar
Pate, J. S. (1975) Exchange of solutes between phloem and xylem and circulation in the whole plant. In Transport in Plants. I. Phloem Transport (Edited by Zimmermann, M. H. and Milburn, J. L.), Encycl. PI. Physiol., New Series, Vol. 1, pp. 451–473. Springer, New York.Google Scholar
Pathak, M. D. (1968) Ecology of common insect pests of rice. A. Rev. Ent. 13, 257–294.CrossRefGoogle Scholar
Sogawa, K. (1973) Feeding of the rice plant- and leafhoppers. Rev. Pl. Protein Res. 6, 31–43.Google Scholar
Wiegert, R. G. (1964) The ingestion of xylem sap by meadow spittlebugs, Philaenus spumarius (L.). Am. Midi. Nat. 71, 422–428.Google Scholar
Ziegler, H. (1975) 3. Nature of transported substances. In Transport in Plants. I. Phloem Transport (Edited by Zimmermann, M. H. and Milburn, J. L.), Encycl. Pl. Physiol., New Series, Vol. 1, pp. 59–100. Springer, New York.Google Scholar