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Electrophoretic study of glutamate dehydrogenase and other enzymes of Nephotettix virescens (Homoptera: Cicadellidae) populations from india, Sri Lanka and the Philippines

Published online by Cambridge University Press:  19 September 2011

G. F. Rajendram
G. F. Rajendram
Affiliation:
School of Pure & Applied Biology, University of Wales, Cardiff CF1 3TL, United Kingdom
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Abstract

Polyacrylamide gel electrophoresis was carried out on Nephotettix virescens (Distant) adults collected from India, Sri Lanka and the Philippines, and maintained in glass-houses on rice variety TN-1 at the University of Wales, Cardiff, United Kingdom. The following enzymes showed activity and were recorded: acid phosphatase (ACP), a-glycerophosphate dehydrogenase (aGPD), fumarase (FUM), glucose-6-phosphate dehydrogenase (G-6-PDH), glutamate dehydrogenase (GLUD), glutamate oxalo-acetate transaminase (GOT), hexokinase (HK), lactate dehydrogenase (LDH), malic enzyme (ME) and xanthine dehydrogenase (XDH).

The bands in nine enzymes were homologous across the three populations and showed no differences.The relative mobility of the enzyme GLUD in the Philippines population was lower than in the other two populations and could be used for diagnostic separation.

The results confirm previous reports of differences in the relative mobility of esterase bands of the Philippines population of N. virescens from India and Sri Lanka populations on starch gel.

Résumé

L'electrophorese surgel polyacrylamide aété fait sur Nephotettix virescens (Distant) adultes rassemblés de l'lnde, Sri Lanka et de Philippines, et conservés dans les maison de vitrée sur riz variété TN-1 à l'Université de Wales, Cardiff, Royaume Uni. Les enzymes suivantes avaient montré activité et ont été enregistrés: l'acide phosphatase (ACP), la dehydrogenase de phosphate de alpha-glycerol (GPD), la fumarase (FUM), la dehydrogenase de phosphate de glucose (G-6-PDH), la dehydrogenase de glutamate (GLUD), l'oxalo-acetatetransminasedeglutamate(GOT), la hexokinase (HK), la dehydrogenase de lactate (LDH), 1'enzyme de I'acide malique (ME) et la dehydrogenase de xanthine (XDH).

Les bandes dans neuf enzymes étaient homologues dans toutes les trois populations et montrent aucune différence. Mais la mobilité relative de l'enzyme GLUD de la population Philippines était plus base que les deux autres populations et peut être employée pour la séparation diagnostique.

Les resultats confirment rapports anterieurs dans la mobilité relative de bandes de esterase de la population Philippines de N. virescens en comparant avec des populations de l'Inde et de Sri Lanka sur empois gel electrephorese.

Keywords

Nephotettix virescensHomopteraCicadellidaepolyacrylamide electrophoresisenzyme variation
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 13 , Issue 5 , October 1992 , pp. 737 - 740
Copyright
Copyright © ICIPE 1992

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References

REFERENCES

Ayala, F. J. and Powell, J. R. (1972) Allozymes as diagnostic characters of sibling species of Drosophlla. Proc. nat. Acad. Sci. USA. 69, 1094–1096.CrossRefGoogle Scholar
Berlocher, S. H. (1984) Insect molecular systematics. Annu. Rev. Entomol. 29, 403–433.CrossRefGoogle Scholar
Claridge, M. F. (1989) Electrophoresis in agricultural pest research—a technique of evolutionary biology. In Electrophoretic Studies on Agricultural Pests (Edited by Loxdale, H. D. and Hollander, J. D.), pp. 1–6. Systematics Association. Special Volume No. 39, Clarendon Press, Oxford.Google Scholar
Claridge, M. F., Den Hollander, J. and Morgan, J. C. (1985) Variation in courtship signals and hybridization between geographically definable populations of the rice brown planthopper, Nilaparvata lugens (Stal). Biol. J. Linn. Soc. 24, 35–49.CrossRefGoogle Scholar
Harris, H. and Hopkinson, D. A. (1976) Handbook of Enzyme Electrophoresis in Human Genetics. Amsterdam, North-Holland.Google Scholar
Loxdale, H. D., Castenera, P. and Brookes, C. P. (1983) Electrophoretic study of enzymes from cereal aphid populations. I. Electrophoretic techniques and staining systems for characterising isoenzymes from six species of cereal aphids (Hemiptera: Aphididae). Bull. Entomol. Res. 73, 645–658.CrossRefGoogle Scholar
Menken, S. B. J. and Ulenberg, S. A. (1987) Biochemical characters in agricultural entomology. Agric. Zool. Rev. 2, 305–360.Google Scholar
Ozaki, K. (1969) The resistance to organophosphorus insecticides of the green rice Ieafhopper, Nephotettix cincticeps Uhler and the small brown planthopper Laodelphas striatellus Fallen. Rev. Plant Prot. Res. 2, 1–15.Google Scholar
Pashley, D. P. and Johnson, S. J. (1986) Genetic population structure of migratory moths: the velvetbean caterpillar (Lepidoptera: Noctuidae). Ann. Entomol. Soc. Am. 19, 26–30.CrossRefGoogle Scholar
Rajendram, G. F. (1987) Esterase variation in Nephotettix nigropíctus (Stal) population from Australia. In Proceedings of the 6th Auchenorrhyncha Meeting (Edited by Vidano, C. and Arzone, A.), pp. 303–309, Turin, Italy.Google Scholar
Rajendram, G. F. (1989) Starch gel electrophoresis of enzymes of Nephotettix virescens (Homoptera: Cicadellidae) populations from India, Sri Lanka and the Philippines. Vingnanam J. Sci. 4, 8–14.Google Scholar
Rajendram, G. F. (1991) Electrophoretic study of enzymes from a Sogateila furcifera Horvath population from Sri Lanka. Insect Sci. Applic. 12, 275–278.Google Scholar
Shaw, C. R. and Prasad, R. (1970) Starch gel electrophoresis of enzymes—a compilation of recipes. Biochem. Gen. 4, 297–320.CrossRefGoogle ScholarPubMed
Yusof, U.M. (1982) Biological and laxonomic studies on some leafhopper pests of rice in south-east Asia. Ph.D. thesis University of Wales, Cardiff.Google Scholar